简介:目的:探讨nm23,mdm2,p185,p21及p53在骨巨细胞瘤(GCT)的表达及与GCT病理分级和复发的关系。方法:应用SP免疫组织化学方法检测nm23,mdm2,p185,p21p53在52例GCT中的表达(GCT按Jaffe分级,I级15例,II例25例,Ⅲ级12例),结果:52GCT中nm23,mdm2,p185,p21andp53的阳性表达率分别为40.4%(21/52),34.6%(18/52),21.2%(11/52),13.5%(7/52),26.9%(14/52)。其阳性表达与病理分级差异均无显著性(P值分别为0.797,0.871,0.441;0.658;0.699)。nm23,mdm2,p185,p21,p53在复发和无复发的病例中,阳性表达率分别为69.2%(9/13),30.8%(12/39),61.5%(8/13),25.6%(10/39),38.5%(5/13),15.4%(6/39);23.1%(3/13),10.3%(4/29),46.2%(6/13),20.5%(8/39)。nm23,mdm2表达与GCT复发率差异有显著性(P值分别为0.003,0.018),而p185,p21,p53的阳性表达与GCT复发差异无显著性(P>0.05),结论:nm23,mdm2,p185,p21及p53在GCT中的表达与病理分级差异无关,而nm23,mdm2的表达与其复发有关。
简介:摘要目的探讨MDM2 RNA原位杂交(RNA in situ hybridization,RNA-ISH)在非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤(atypical lipomatous tumor/well-differentiated liposarcoma,ALT/WDL)及去分化脂肪肉瘤(dedifferentiated liposarcoma,DDL)中的诊断价值。方法纳入四川大学华西医院病理科2017年3月至2019年5月诊断的ALT/WDL/DDL共26例,对照组病例18例,行MDM2 RNA-ISH,并且与荧光原位杂交(FISH)、免疫组织化学(IHC)结果进行比较。结果所有样本均成功进行上述3种方法学检测。在26例ALT/WDL/DDL中,所有病例均呈MDM2扩增(26/26,100%),MDM2 RNA-ISH均为阳性(26/26,100%)。MDM2 IHC显示24例呈阳性(24/26,92.3%),2例为阴性(2/26,7.7%)。18例对照组病例中MDM2 FISH及MDM2 RNA-ISH均为阴性,15例(15/18)MDM2 IHC阴性。MDM2 RNA-ISH灵敏度及特异度均为100%。MDM2 IHC灵敏度及特异度分别为92.3%和83.3%。在MDM2 RNA-ISH 阳性ALT/WDL/DDL中,所有病例均呈弥漫染色,而在MDM2 IHC阳性病例中,仅8/24(33.3%)呈弥漫阳性。在11例以组织芯片形式评估的ALT/WDL/DDL样本中,MDM2 RNA-ISH阳性比例为11/11,所有病例呈弥漫染色,MDM2 IHC阳性比例为9/11,其中仅1/9病例呈弥漫染色。MDM2 RNA-ISH结果与FISH完全一致,和IHC具有较高的一致性(Kappa=0.763,P<0.001)。结论MDM2 RNA-ISH在ALT/WDL/DDL中与FISH具有高度一致性,灵敏度及特异度均高于IHC,且信号更为弥漫,具有较高的诊断价值。
简介:摘要目的探讨Pokemon蛋白与P14ARF-MDM2-p53通路蛋白在非小细胞肺癌(NSCLC)发病中的作用。方法纳入2015年10月至2016年10月河北医科大学第一医院行手术治疗的35例NSCLC患者为NSCLC组。同时,将癌旁正常支气管断端组织29例及肺炎组织25例分别作为支气管对照组和肺泡对照组。采用免疫组织化学法检测3组中Pokemon、P14ARF、MDM2和p53蛋白的表达情况,分析其与临床病理资料的关系及各蛋白间的相关性。结果Pokemon、MDM2、p53蛋白在NSCLC组中的表达均显著高于支气管对照组和肺泡对照组(P值均<0.01),P14ARF蛋白在NSCLC组中的表达显著低于支气管对照组和肺泡对照组(χ2=25.39,P<0.01)。p53和MDM2蛋白的表达与NSCLC的分化程度相关(P=0.02、0.03)。在NSCLC组中,Pokemon与P14ARF蛋白表达呈负相关性(r=-0.58,P<0.05),P14ARF与MDM2蛋白表达呈负相关性(r=-0.51,P<0.05),MDM2与p53蛋白的表达呈正相关性(r=0.86,P<0.05)。结论Pokemon通过P14ARF-MDM2-p53通路在NSCLC发病过程中发挥癌基因作用。
简介:AbstractObjective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen (2H/1H) exchange at the metabolic level upon exposure to heavy water (2H2O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water. The treated mouse was fed with 2H2O (80%, v/v) and the control mouse fed with standard drinking water (H2O) over next 13 days. Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1, 2, 3, 10, 12, and 13. Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University (approval No. 20161117163322) on October 29, 2021.Results:We observed a replacement of 1H by 2H in 52 mass spectral features (60 2H/1H isotopologue pairs) for the mouse fed with 2H2O, but not for the control mouse. These included pyruvic acid and lactic acid, lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid, propionic acid, butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo2H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use 2H tracing to extend current metabolic studies, especially those with a focus on anaerobic glycolysis, lysine methylation and gut microbiome via monitoring of short-chain fatty acids.
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简介:BackgroundTaxifolin(Tax)isanessentialnaturalantioxidant.MultiplestudieshaveshownthatTaxcanprotectcardiomyocytesfromischemia-reperfusioninjury.However,theunderlyingmechanismisstillunclear.MethodsH9C2cellswererandomlydividedintocontrol,H_2O_2group,Taxpretreatmentgroup(Tax+H_2O_2);Taxeffectgroup.CellactivitywasdetectedbyCCK-8andtheintracellularstructurewasobservedbytransmissionelectronmicroscopy.AutophagywasdeterminebyWesternblottinganalysisofBeclin-1,Bcl-2andPKC.ResultsTaxpretreatmentsignificantlyincreasedanti-apoptoticproteinBcl-2andautophagyproteinBeclin-1.ExpressionofPKCwasinhibitedbyTax.ConclusionsTaxpretreatmentcouldprotectH9C2cellsagainstH_2O_2-induceddamagethroughtheBcl-2andautophagypathways.
简介:摘要目的朗迈2(LabUmat2+Urised2)全自动尿液分析仪是LabUmat2尿液干化学分析仪和Urised2尿液有型成分分析仪通过同一标本运输轨道联机检测尿常规的全自动仪器。检测项目多、速度快、精密度高,尿液有形成分分析结合人工显微镜检查准确度高。方法LabUmat2全自动尿液干化学分析仪反射光度计法,折射仪法。Urised2全自动尿液有型成分分析仪自动离心镜检,影像式神经网络智能识别系统检测。结果LabUmat2分析仪,配用专用的尿液分析试纸条,检测尿液化学成分;Urised2分析仪检测尿液有形成分。结论朗迈2(LabUmat2+UriSed2)全自动尿液分析仪应用全过程严格按照检测前、检测中、检测后的质量保证体系做好全程质量控制,可及时、准确获得尿常规报告,为临床诊断、疗效监测、预后判断提供可靠依据。同时也是临床实验室尿常规检测技术的提高。
简介:RecombinanthumanGABAAreceptorswereinvestigatedinvitrobycoexpressionofcDNAscodingforα1,β2andγ2subunitsinthebaculovirus/Sf-9insectcellsystem,Asingleaminoacidexchangeα1(asparaticacid151toasparaginorα1(threonine149toglutamine)intheN-terminal,extracellularpartoftheα1subunitinducedabout10folddecreaseinanantagonistpitrazepineaffinity.OtherGABAAreceptorligandshadlittledifferenceintheiraffinity.Itwaslikelythat151and149aminoacidresidueswereessentialforthebindingaffinityandefficacyofpitrazepinetoGABAAreceptorcombinationscontainiαααααααααnganα1subunit.
简介:目的评价CHADS22及CHA2DS2-VASc评分系统在冠心病外科治疗中的意义.方法选择2006年1月至2010年1月行不停跳冠状动脉旁路移植术的768例患者,术后新发房颤患者97例.回顾患者的围术期及随访资料,应用CHADS2及CHA2DS2-VASc评分系统进行分析.结果768例患者术后新发房颤发生率12.6%,分为术后新发房颤组与非房颤组.新发房颤组与非房颤组平均年龄分别为(70.74±8.21)岁和(65.90±9.83)岁,围术期脑卒中分别为8例和9例,CHADS2评分值分别为3.20±1.26和2.13±0.94,CHA2DS2-VASc评分值分别为4.20±1.50和3.23±1.07.CHADS2和CHA2DS2-VASc评分是术后新发房颤的预测因素,与围术期脑卒中显著相关(P<0.01).结论冠心病外科治疗中应用CHADS2及CHA2DS2-VASc评分系统可预测术后新发房颤及围术期脑卒中,对冠心病术后新发房颤的抗凝及抗血小板治疗决策提供了依据,对卒中风险及预后有一定的评估价值.