简介：Smallubiquitin-likemodifier(SUMO)-conjugatingenzymesareinvolvedinpost-translationalregulatoryprocessesineukaryotes,includingtheconjugationofSUMOpeptidestoproteinsubstrate(SUMOylation).SUMOylationplaysanimportantroleinimprovingplanttolerancetoabioticstresssuchassalt,drought,heatandcold.Herein,wereportedtheisolationofOsSCE1(LOC_Os10g39120)geneencodingaSUMO-conjugatingenzymefromrice(Oryzasativacv.Nipponbare)anditsfunctionalvalidationinresponsetodroughtstress.TheE2enzyme,OsSCE1,isoneofthreekeyenzymesinvolvedintheconjugationofSUMOtoitstargetproteins.ActivatedSUMOistransferredtothecysteineofanE2enzymeandthentothetargetlysineresidueofthesubstrate,withorwithoutthehelpofanE3SUMOligase.ExpressionofOsSCE1wasstronglyinducedbypolyethyleneglycol6000(PEG6000)treatment,whichsuggestedOsSCE1maybeinvolvedinthedroughtstressresponse.OverexpressionofOsSCE1(OsSCE1-OX)inNipponbarereducedthetolerancetodroughtstress.Conversely,thedroughttolerancewasslightlyimprovedbytheknockdownofOsSCE1(OsSCE1-KD).TheseresultswerefurthersupportedbymeasurementofprolinecontentinOsSCE1-OXandOsSCE1-KDtransgeniclinesunderinduceddroughtstress,whichshowedOsSCE1-KDtransgeniclinesaccumulatedhigherprolinecontentthanthewildtype,whereasOsSCE1-OXlinehadlowerprolinecontentthanthewildtype.ThesefindingssuggestedOsSCE1mayplayaroleasanegativeregulatorinresponsetodroughtstressinrice.

简介：Bacterialleafblightofrice(BLB),causedbyXanthomonasoryzaepv.oryzae,isoneofthemostdestructivediseasesinAsianricefields.Ahigh-qualityricevariety,LT2,wasusedastherecipientparent.IRBB21,whichcarriestheXa21gene,wasusedasthedonorparent.TheresistancegeneXa21wasintroducedintoLT2bymarker-assistedbackcrossing.ThreeXooraceswereusedtoinoculatetheimprovedlinesfollowingtheclippingmethod.ElevenBC3F3linescarryingXa21wereobtainedbasedonmolecularmarkersandagronomicperformance.The11linesweretheninoculatedwiththethreeXooraces.Allthe11improvedlinesshowedbetterresistancetoBLBthantherecipientparentLT2.BasedonthelevelofresistancetoBLBandtheiragronomicperformance,fivelines(BC3F35.1.5.1,BC3F35.1.5.12,BC3F38.5.6.44,BC3F39.5.4.1andBC3F39.5.4.23)wereselectedasthemostpromisingforcommercialrelease.Theseimprovedlinescouldcontributetoriceproductionintermsoffoodsecurity.