简介:AIM:Todescribethedesignandpreliminaryresultsofthehospitalbasedepidemiologicalstudyfordiabeticretinopathy(HBESDR),anongoingepidemiologicalstudytoestimatetheprevalenceofdiabeticretinopathy(DR)andtoelucidatetheclinical,anthropometric,biochemicalandanyotherriskfactorsassociatedwithdiabeticretinopathy.METHODS:Totally2000diabeteswillberecruitedfromtheDiabeteseyeclinicintheFirstAffiliatedHospitalofChinaMedicalUniversity.AllsubjectsunderwentbloodsugarestimationandOralGlucoseToleranceTesttodiagnosediabetes.Alldiabeteswouldundergocompletequestionnaire,acomprehensiveeyeexamination.Bloodandurinewouldbecollectedforbiochemicalinvestigations.AllfundusphotographsforanyDRwillbegraded.Participantswhoneedtreatmentwillbesenttotheophthalmicclinicandfollow-upintervalprogramforallsubjectswillbesuggested.Acomputerizeddatabaseiscreatedfortherecords.RESULTS:Todate,1174diabeteshavebeenrecruited,therewere350(29.81%)DRinalldiabetes,mostofthemwerewithmildnon-proliferativediabeticretinopathy(NPDR)(139,39.71%);71(20.29%)moderateNPDR,66(18.86%)severeNPDR,74(21.14%)proliferativediabeticretinopathy(PDR).Females,longerdurationofdiabetes,familyhistoryofdiabetesandhypertensionhadastatisticallysignificantincreaseinriskofanyDR.CONCLUSION:ThestudyisexpectedtoprovideanestimateoftheoverallprevalenceofDRandtheprevalencewithdifferentdurationofdiabetesandalsoabetterunderstandingoftheriskfactorsassociatedwithDR.
简介:目的:研究如何将诱发电位技术应用于视力的客观评定。方法:视力范围0.1~1.5志愿合作受试者共180眼,运用模式翻转视觉诱发电位(PRVEP)技术检测与记载各受检眼各不同信号视角条件下的结果,探究可靠的检测指标。应用SPSS软件对收集数据作统计学处理和分析。结果:确定最小信号视角是客观评估视力的基础,选定P100波幅作为视力量化评价指标。通过对一定条件下P100波幅与视力表视力进行简单相关回归分析,显示二者呈正相关。结论:最小信号视角、P100波幅可作为确切的检测指标。
简介:目的探讨桥小脑角疾病的相位平衡快速梯度回波(Balance-FastFieldEcho,B-FFE)序列MRI影像学特点,评价B-FFE序列对桥小脑角疾病的诊断价值.方法应用B-FFE序列对136例桥小脑角病变者(三叉神经痛131例、半面痉挛5例)进行MRI检查,观察桥小脑角神经、血管、肿瘤显像情况,并与手术中资料进行比较.结果131例三叉神经痛患者中,117例为原发性三叉神经痛,MRI显示有血管压迫者102例,术中证实有血管压迫者95例,行显微血管减压+神经梳理术;14例为继发性三叉神经痛,均为桥小脑角占位性病变,术后病理证实7例为胆脂瘤,2例为听神经瘤,5例为脑膜瘤;5例半面痉挛患者MRI显示有血管压迫,术中证实有血管压迫,并予以血管减压.结论B-FFE序列MRI成像能清楚显示桥小脑角区三叉神经、面神经与血管的关系及是否有占位性病变,对诊断、术前评估和指导治疗有重要意义.
简介:回顾分析经病理检查证实的真菌性鼻窦炎32例,探讨其螺旋CT征象。
简介:·AIM:Toinvestigatethetherapeuticeffectsofnanophtha-locyaninephotosensitizersonanexperimentalratchoroidalneovescularization(CNV)model,aswellastoevaluatethecytotoxicityofwhichonhumanretinalpigmentepithelia(HRPE)andhumanretinalendothelialcells(HRECs).·METHODS:Twotypesofphotosensitizers,G1-ZnPc(COOH)8andG1-ZnPc(COOH)8/mrespectively,wereadministratedforphotodynamictherapy(PDT)afterasuccessfulestablishmentofCNVmodelonBrown-Norway(BN)ratsviafundusphotocoagulation.Thetherapeuticeffectsofthetwodrugswereassessedthroughopticalcoherencetomography(OCT),fluoresceinfundusangiography(FFA)andtransmissionelectronmicroscopy(TEM).Forcytotoxicitytests,cellcountingkit-8(CCK-8)assaysandchangesofmitochondrialtransmembranepotential(△Ψm)wereconductedonHRPEandHRECsafterinitialuptakeofthetwodrugs.·RESULTS:Bothphotosensitizersdemonstratedanimprove-mentofvascularleakageandclosureofCNV1weekafterPDTasconfirmedbyfundusimage,OCT,FFAandTEM.TwoweeksafterPDT,G1-ZnPc(COOH)8/mshowedabetterCNVclosureeffectversusG1-ZnPc(COOH)8(P<0.05).Asignificantdifference(P<0.01)wasfoundinuptakeofthetwodrugsinHRPEandHRECs,withnodifferencebetweenthedrugs(P>0.05).BothphotosensitizersshowedcytotoxicityonHRPE,butG1-ZnPc(COOH)8/minducedalowercellviability.·CONCLUSION:G1-ZnPc(COOH)8/mmediatedPDTisbetterthanG1-ZnPc(COOH)8inCNVclosureandalsohavetheadvantageoffastmetabolismleadingtolesssideeffect.·
简介:目的探讨缩短小切口非超乳白内障摘除人工晶体晶植人术学习曲线的途径.方法对2006年萧县彭年光明行动中完成29例小切口非超乳白内障摘除人工晶体晶植入术的病例进行回顾性分析.结果通过最先完成17例小切口非超乳白内障摘除人工晶体晶植入术部分手术操作步骤,循序渐进最后独立完成12例小切口非超乳白内障摘除人工晶体晶植入术的全部操作步骤.结论熟练掌握白内障囊外摘除+人工晶体植入技术,是缩短小切口非超乳白内障摘除+IOL植入学习曲线的关键因素.
简介:目的观察初学者学习0.7毫米微创白内障手术改良法的临床效果。方法随机选取门诊和住院白内障患者50人,由初学者在老师指导下应用0.7毫米微创白内障手术改良法施行手术。研究内容包括手术时间,术中灌注液应用量,术中并发症等。结果手术时间随着学习的进程总体呈下降趋势,从开始10例平均时间61分22秒下降到第5个10例的18分54秒。特别是30例后下降明显。共7例9次并发症发生。第4例出现后囊破裂,未行玻璃体切割的情况下成功植入人工晶状体于睫状沟内。前10例中共有2例,第2和4个10例中各1例术中角膜上皮水肿。另外还有1例发生后弹力层撕裂,两例切口撕裂,1例结膜水肿。结论经过短期的0.7毫米微创白内障手术改良法训练,初学者可从传统同轴超声乳化安全转换至0.7毫米微创白内障手术。
简介:AIM:Todeterminethenormativevaluesofwhite-towhitecornealdiameterwithOrbscanIITopographySystemandtocomparerightandlefteyesdatainthenormalyoungpopulation.METHODS:Atotalof1001healthyparticipantsaged18-45yparticipatedinthisobservationalcross-sectionalstudy.Thestudypopulationconsistedof616femaleand385malesubjects.ThecornealdiameterwasmeasuredwiththeOrbscanII.Thedifferencesbetweengenders,betweenrightandlefteyesandage-relatedchangeswereevaluated.StatisticalanalyseswereperformedusingStudent’st-test.RESULTS:Theaveragewhite-to-whitedistanceinourstudypopulationwasrecordedas11.65±0.36mm(median:11.60mm,mode:11.70mm,minimum:10.50mmandmaximum:13.60mm).Thewhite-to-whitedistancewas11.60±0.35mminmalesand11.71±0.36mminfemaleswhichwasstatisticallydifferentbetweengenders(P<0.01).However,white-to-whitedistancewasnotstatisticallydifferentbetweenrightandlefteyes.Inaddition,thisparameterdecreasedwithincreasingage.Considering95%confidenceinterval,cornealdiameterlessthan10.93mmandgreaterthan12.34mmwouldbeconsideredasmicrocorneaandmegalocornea,respectivelybasedonthisstudypopulation,usingtheOrbscanIItopography.CONCLUSION:DetaileddescriptionandanalysisofcornealdiameterwithOrbscandemonstratethattheobtainedaveragevalueofhorizontalwhite-to-whiteishigherinmalethanfemaleanddecreasesslightlywithincreasingage.Ourdataalsosuggeststhecutoffvaluesfordefinitionofmicrocorneaandmegalocornea,whichcanbeemployedwiththispopulation.
简介:AIM:Todeterminethehistopathologicalchangesofrifampicinappliedintravitreallyonretinalganglioncellsbymeansofstereologicalandhistopathologicalmethods.METHODS:Forthisstudytwenty-fourNewZealandadultrabbitsweredividedintofourgroups(n=6foreachgroup).50μg/0.1mL(group1),100μg/0.1mL(group2),150μg/0.1mL(group3)and200μg/0.1mL(group4),rifampicinwereinjectedintothevitreousoftherighteyesofanimals,theirlefteyeswereusedascontrol(group5).Afterthe28thdayofapplication,animalswereanesthetisedwithxylazine(8mg/kg,IM)andthentheireyeswereenucleatedimmediately.Patternsweretakenawayandeyeswerepreparedforbothstereologicalandelectromicroscopicobservation.RESULTS:Dependingonthehighdoseofrifampicin,somehistopathologicalchangessuchascytoplasmicdilatationanddamagedmembranewereobservedontheelectromicroscopiclevel.Usingquantitativeexamination,whichwasdoneatthelightmicroscopiclevel,itwasshownthatthenumberofneuronsdecreasedlinearlyasrifampicindoseincreasedwhencomparedwiththecontrolgroup.CONCLUSION:Basedonthesefindings,low-doserifampicin(50μg/0.1mL)maybeusefulfortreatmentoftheoculardiseases.
简介:·AIM:Toinvestigatetheantifibroticeffectoffreeze-driedbilayeredfibrin-bindingamnioticmembraneontrabeculectomyinarabbitmodel.·METHODS:Twenty-fourJapanesewhiterabbitswererandomizedintothreegroups:theexperimentalgroup(oculartrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembranetransplantation),thecontrolgroup(oculartrabeculectomyincombinationwithnaturalbilayeredfibrin-bindingamnioticmembrane)andtheblankgroup(singletrabeculectomy).Clinicalobservation,hematoxylin-eosinstaining,Massionstaining,real-timePCRandimmunohistochemistryforα-SMAwereperformedondays7,14,21and30followingsurgery.·RESULTS:Statisticaldifferenceswerenotedinsurvivalanalysisandintraocularpressure(IOP)amonggroupsondays7,14,21and30followingsurgery.Histology,immunoh-istochemistryandreal-timePCRfurtherdemonstratedthattrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembraneresultedingoodwoundhealingandnoscarformation.·CONCLUSION:Self-madefreeze-driedbilayeredfibrin-bindingamnioticmembranemayinhibittheformationofscarringinglaucomaaftertrabeculectomy.·
简介:AIM:Toadaptthelowvision-relatedqualityoflife(LVQOL)instrumentintoTurkishlanguageandtoassessitsvalidityandreliability.METHODS:Thestudywasconductedin387patientsattendingtheCentreofLowVisionRehabilitation,FacultyofMedicine,AnkaraUniversity.Forstatisticalanalyses,theSpearman’scorrelationcoefficient,Cronbach’salphacoefficientandConfirmatoryFactorAnalysis(CFA)wereused.RESULTS:AccordingtoresultsofCFA,theiteminthe'Adjustment'subscalebecauseofhavingthefactorloadingbelow0.40,wasexcludedfromthequestionnaire.ThereliabilityofthequestionnairewasassessedaccordingtoCronbach’salphacoefficients.Thereliabilityofthe'DistanceVision,Mobility,andLighting'subscalewas?琢=0.863;ofthe'Adjustment'subscalewas?琢=0.694;'ReadingandFineWork'was?琢=0.791,and'ActivitiesofDailyLiving'was?琢=0.770.Sotheseresultsindicatethatthequestionnaireisreliabletomeasurethevisionrelatedqualityoflifeoflow-visionpatients.Thecorrelationsbetweenthesubscaleswerealsoanalyzed,andthecorrelationbetween'Adjustment'and'ReadingandFineWork'wasfoundtobethelowest(rs=0.336,P<0.001),whereasthestrongestcorrelationwasfoundbetweenthe'ReadingandFineWork',and'ActivitiesofDailyLiving'.Additionally,the'Adjustment'dimensionshowedthestrongestcorrelationwithonly'DistanceVision,Mobility,andLighting'dimension.CONCLUSION:Afterremovingthelastitemintheseconddimension,theTurkishadaptationofalldimensionsoftheLVQOLhasbeenshowntobereliable,validandsuitableforuseinpatientswithlowvisioninTurkey.
简介:AIM:ToevaluatetheeffectofCollagencross-linkingonthepreventionofmeltinginrabbitcorneasafteralkaliburn.·METHODS:TwentyNewZealandwhiterabbitswererandomlydividedintomodelcontrolgroupandcollagencross-linkingtreatmentgroup.Thesecondgroupofrabbitsreceivedcollagencrosslinkedtreatment.Bothgroupswereappliedwithantibioticeyedropstopreventinfection.Thecorneaswereevaluatedformelting,opacity,pathologicalandimmunohistochemistry,recordthechangeswhen28daysaftertheanimalswerekilled.·RESULTS:Inthecontrolgroup,6outof8rabbitsshowedcornealmeltingafterinjury(14±4)days,whiletwocornealperforated.Incollagencross-linkingtreatmentgroup,onerabbitshowedcornealmeltingafterinjury23days,withoutcornealperforation;cornealdissolutionratebetweenthetwogroupswassignificantlydifferent(P<0.05).Pathologicalexaminationsuggestedthatinthetreatmentgroup,mildcornealedema,milddamagetocollagenfibers,inflammatorycellinfiltrationwassignificantlylessthanthecontrolgroup.Immunohistochemistryshowedthatcornealcollagenfibersarrangedinneatrowsinthecontrolgroup.·CONCLUSION:Collagencross-linkingtreatmentnotonlycanpreventanddelaythecornealmeltingafteralkaliburn,butalsocanreducethedestructionofcornealcollagenfibersandinfiltrationofinflammatorycellsinthecornealtissue.