简介:目的分析复合式小梁切除术对晚期青光眼的治疗效果。方法2014年7月~2016年7月,在我院治疗晚期青光眼的患者中随机抽取89例作为本研究的分析对象,并按其选择的术式将其分为两组(治疗组45例与参照组44例)。对治疗组与参照组患者分别给予复合式小梁切除术与传统小梁切除术进行治疗。手术结束后,将两组患者的浅前房发生率、眼压控制情况以及并发症发生情况进行比较。结果治疗组浅前房发生率(8.89%)明显低于参照组(29.55%),且χ~2=6.14,P〈0.05,差异存在统计学意义。术前与术后1个月,两组患者的眼压均无显著差异(P〉0.05),无统计学意义;而术后6个月与术后12个月,治疗组患者的眼压均显著低于参照组(P〈0.05)。治疗组的并发症发生率显示为8.89%,参照组为25.00%,前者低于后者(χ~2=4.12,P〈0.05),差异具有统计学意义。结论对于晚期青光眼患者而言,复合式小梁切除术是最佳的治疗方法,该方法具有确切的治疗效果,并能够将患者的浅前房发生率与眼压进行有效地降低,同时还能够减少并发症的发生情况,值得推广。
简介:目的探讨全视网膜冷凝联合睫状体冷冻术治疗晚期新生血管性青光眼的疗效。方法对2011年1月到2013年2月间在我院住院的18例18眼晚期新生血管性青光眼患者使用全视网膜冷凝加睫状体冷冻术治疗的临床资料进行回顾性分析。结果所有患者出院后随访12-15月,15眼眼压控制正常,虹膜表面新生血管消退,成功率达83%。2眼术后眼压在31-34mmHg,给予贝特舒眼药水后眼压控制正常。1眼术后眼压大于35mmHg,一月后给予第2次冷冻治疗,术后眼压控制正常。结论全视网膜冷凝联合睫状体冷冻治疗晚期新生血管性青光眼不仅是一种简单、经济、而且是一种控制晚期新生血管性青光眼的眼压,解除患者临床症状的有效方法。
简介:目的探讨复合式小梁切除联合晶状体摘除及人工晶体植入术治疗中晚期闭角型青光眼的临床疗效。方法对诊断中晚期闭角型青光眼的患者86例(94眼)随机分成2组。A组48例(52眼)行复合式小梁切除联合晶状体摘除及人工晶体植入术,B组38例(42眼)行传统小梁切除术,对其术后视力、眼压、滤过泡、前房情况进行观察及处理。结果术后第1天浅前房发生率A组为3.85%,明显低于B组30.95%(P〈0.05)。随访12个月,A组功能性滤泡占84.62%,较B组功能性滤泡66.67%为多(P〈0.05)。以不用降眼压药眼压控制在21mmHg,以下为手术成功标准。A组手术成功率92.31%,较B组69.05%为高(P〈0.05),矫正视力〉0.05的眼数A组为65.38%,明显好于B组30.95%(P〈0.05)。结论复合式小梁切除联合晶状体摘除及人工晶体植入术是治疗中晚期闭角型青光眼实用有效的手术方法,较传统的小梁切除术相比,手术成功率高,并发症少。
简介:AIM:Todeterminetheproliferativepotentialandthemaintenanceofstemcellactivityinstoredhumanlimbaltissues,andcorrelatethiswiththepreservationtime,cellviabilityandtheexpressionofstemcellmarkers.METHODS:Thirtylimbalrimsweresplitinto4partsandstoredincornealpreservationmediumat4℃for0,1,4,or7days.ThelimbalstemcellandmitoticmarkersP63,CK19,proliferatingcellnuclearantigen(PCNA),andKi67weredeterminedbyimmunohistochemicalstaining.Theproliferativepotentialoflimbalepithelialcellswasassessedbycellviability,theabilityofgeneratingstratifiedepithelium,andcolonyformingassay.RESULTS:Thestoredtissuesmaintainedlimbalstratifiedstructureto7daysandexhibitedcomparableexpressionlevelofstemcellandmitoticmarkers.Theproportionofviablecellsdecreasedwiththeprolongedpreservationtime,whilecolonyformingefficiencydecreasedfromthe1stdayanddisappearedatthe4thday.Wheninoculatedonamnioticmembrane,thecellspreservedfor1dayformedastratifiedepithelium,whilethecellsfrom4days’preservationformedadiscontinuouslayer.CONCLUSION:Thecolonyformingefficiencyoflimbalepithelialstem/progenitorcellsdecreasedrapidlywiththeincreasingpreservationtime,whiletheexpressionlevelofmarkersandcapacityofformingepithelialmonolayeronamnioticmembranedecreasedgradually.Thelimbalepithelialstemcellslosttheirfunctionearlierthanthelostexpressionlevelofstemcellmarkers.Thismayhelpustobetterchoosetheappropriatepreservationgraftsforfuturelimbalstemcelltransplantation.
简介:病例介绍病例1:患者,男,47岁。因右眼突然视力下降伴下方黑幕遮挡4天入院。原双眼有高度近视-14.00DS,入院时全身体检(-)。视力:右眼无光感,陈旧性视网膜脱离;左眼0.05(矫正)。左眼前节(-),眼底见9:30~4:00视网膜脱离,隆起度较高,累及黄斑区,颞上方约1:00时钟见一2PD大小马蹄孔。入院后第二天局部麻醉下行左眼冷凝加外加压加环扎术,外加压块、环扎带均位于赤道区,无放液并发症,手术顺利。术后第一天查眼底见脱离的
简介:AIM:Topresentretinalmicrostructure,metabolismandfunctionabnormalitiesinthecourseofmultipleevanescentwhitedotsyndrome(MEWDS)byHeidelbergspectralismodalityimagingplatformandobserveitsoutcomebyEDI-SD-OCTandtwowavelengthautofluorescence.METHODS:Acaseofmultipleevanescentwhitedotsyndromeina23-year-oldfemalepresentedinitiallywitha15-dayhistoryoffloatersandacentralscotomaintherighteye.Toestablishthediagnosis,multimodalityimagingwasperformed,namely,bluelight-fundusautofluorescence(BL-FAF,excitation488nm,emission>500nm),near-infraredfundusautofluorescence(NIR-FAF,excitation787nm,emission>800nm)usingaconfocalscanninglaserophthalmoscope,fundusfluoresceinangiography(FFA),indocyaninegreenangiography(ICGA),spectrum-domainenhancedepthimagingopticalcoherencetomography(SD-EDI-OCT),multifocalelectroretinography(mf-ERG)andfundusphotograghwereperformedandfollowedupattheeighthmonthafterinitiallyvisiting.RESULTS:Opticalcoherencetomography(OCT)showedatransientdisruptionofthefovealphotoreceptoroutersegmentsincorrespondencetofovealgranularity.NIR-FAFshowedhypoautofluorescentareas,≤40μminsize,mostlyconcentratedaroundtheposteriorpoleanditstemporalsidelessthanthatinBL-FAF.Mf-ERGshowpinnacledisappearedinfoveaandmaculaandresponsesdecreasedmarkedlycomparedwiththefolloweye.Attheeighthmonthfollowup,hyperfluorescenceinBL-FAFweredisappear,while,NIR-FAFHypofluorescentspotsinearlystageofsuchlesionwerereduced.ButOCTdemonstratedthestructurewasrecoveredinresidualHypofluorescentareainNIR-FAF.Thesubfovealchoroidalthicknesswasdecreasedfrom372μmto307μmslightlyandcostlinewasrecovered.CONCLUSION:MEWDSisabenignself-healingdiseaseandthereisnopathologicalevidencetoinvestigatethenaturalcourseofsuchdisease.SD-OCTallowshighlydetailedimagesapproachinghistopathologytocertifythemicrostructura
简介:·AIM:Tostudytheeffectsofdanhonghuayukoufuye(DHK)onfastingbloodglucose(FBG)anddiabeticretinopathy(DR)instreptozotocin(STZ)-inducedtype1diabeticratstofacilitatetherationalusageofthisdrug.·METHODS:DiabeticratswereinducedbyinjectionofasingledoseofSTZintraperitoneallyat50mg/kg.Flashelectroretinogram(FERG)andoscillatorypotentials(OPs)wereusedtomeasureretinalfunction.Themicrovascularperfusionofearswasperformedtostudythemicrocirculationinrats.FBG,body-weight,and24-hurinevolume,waterintakeanddietintakewerealsoassessed.·RESULTS:DHKhadnoeffectonFBGinnormalrats.However,STZ+DHKgroupweresignificantlydifferentfromthoseofModelandmovedtowardthoseofnormalcontrol.Itreversedtheincreaseindietintake(P≤0.05vsmodelcontrol)andthelossinbody-weight(P≤0.05vsmodelcontrol)indiabeticrats.DHKdecreasedtheFBGofdiabeticratsby25.6%(P≤0.05)and37.9%(P≤0.01)after14and21daysadministrationascomparedwiththemodelcontrol,respectively.Moreover,DHKsignificantlyincreasedtheFERGb-waveamplitudeby80%(P≤0.05vsmodelcontrol)anddecreasedtheFERGb-wavelatencyby15.3%(P≤0.01vsmodelcontrol)after24daysadministration.TheOP1andOP2amplitudesinDHKgroupwere2.6(P≤0.01)and2.0(P≤0.01)timesofmodelgroupafter24daysofDHKtreatment,respectively.Atthesametime,OP1andOP2latenciesinDHKgroupreducedby16.0%(P≤0.001)and14.7%(P≤0.001)ascomparedwiththemodelcontrol,respectively.Furthermore,themicrovascularperfusionofDHKgroupwas2.4timesofmodelgroup(P≤0.001)after21daysadministration.·CONCLUSION:DHKhadnoeffectonnormalFBG.Butithadantihyperglycemicactivity,andhadapreventiveandtherapeuticeffectonDRindiabeticrats.·
简介:目的:探讨改良式额肌腱膜瓣悬吊术矫治儿童重度上睑下垂的长期临床疗效。方法:回顾性分析2009-01/2011-12在我科收治的儿童重度上睑下垂患者83例114眼,采用改良式扇形额肌腱膜瓣悬吊术进行矫治,分析术后上睑下垂矫正效果和上睑缘弧度、重睑形成及倒睫、结膜脱垂等并发症发生情况。随访观察5a。结果:术后第5a,矫正满意和基本矫正者共有84眼(73.7%),部分矫正30眼(26.3%),手术效果满意。术后并发症包括倒睫4眼(3.5%),其中3例3眼上睑内侧1/3灰线处缝牵引缝线牵引lwk后消失,1例1眼行二次手术;结膜脱垂2眼(1.8%),应用皮质类固醇激素眼药水点眼,加压包扎lwk后结膜脱垂均消失;术后无1例发生暴露性角膜炎、眼睑外翻、睑球分离、眼睑成角畸形、血肿形成、感染等并发症。术后上睑回退和外侧重睑皱襞消失是长期随访最常见的问题。结论:改良式额肌腱膜瓣悬吊术矫治儿童重度上睑下垂长期疗效满意,效果持久稳定,安全可靠。
简介:AIM:ToinvestigatetheeffectofY-27632onthesurvivalandneuriteoutgrowthoftheculturedretinalneurocytes.METHODS:Afterthepostnatalday2-3,Sprague-Dawleyretinalneurocyteswereculturedfor48hours,theculturemediawasreplacedwithserum-freemedia(controlgroup)andserum-freemediacontained30μmol/LY-27632(Y-27632group),andthecellswerecontinuallyculturedanother48hours.Theculturedretinalneurocyteswereidentifiedwithanti-neuronspecificenolase(NSE)immunocytochemistry.ThesurvivalstateofthosecellswasestimatedbyMTTassay,andtheneuriteoutgrowthofthosecellswasevaluatedbythecomputerizedimage-analysissystem.RESULTS:Comparedwiththecontrolgroup,theabsorbancevaluesofcellssurvivalinY-27632groupincreased12.90%and33.33%respectivelyafter72and96hoursculture.Y-27632hadnosignificanteffectonthediameterofculturedretinalneurocytes.Comparedwiththecontrolgroup,Y-27632inducedastableimprovementofneuriteoutgrowthofretinalneurocytesafter72and96hoursculture(P=0.001).CONCLUSION:Y-27632couldpromotethesurvivalandneuriteoutgrowthoftheearlypostnatalculturedretinalneurocytes.