简介：客观Plasminogen使活跃之物inhibitor-1(PAI-1)，plasminogen使活跃之物系统的一个关键部件，是在许多脉管的疾病的致病以及在癌症的一个主要播放器。是在许多脉管的疾病的致病以及在癌症的一个主要播放器。在乳癌组织的PAI-1的高水平与差的预后被联系。癌症织物与差的预后被联系。这研究的目的是严厉地评估作为诊断或预示的试金的一般屏蔽测试工作的浆液PAI-1集中的潜力。作为诊断或预示的试金的一般屏蔽测试工作的PAI-1集中。

简介：ＡＮＴＩＣＯＭＰＬＥＭＥＮＴＡＲＹＡＣＴＩＶＩＴＹＩＮＨＵＭＡＮＳＥＲＵＭＯＦＬＵＮＧＣＡＮＣＥＲＰＡＴＩＥＮＴＳＡＮＤＩＴＳＰＯＳＳＩＢＬＥＣＬＩＮＩＣＡＬＳＩＧＮＩＦＩＣＡＮＣＥＬｉｕＨｕｉｒｏｎｇ刘慧荣ＬｉａｎｇＦｅｎｇ梁峰ＺｈａｎｇＷｅｉｆ...

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简介：Objective:Hepatocellularcarcinoma(HCC)isaleadingcauseofcancer-relateddeaths.NovelserumbiomarkersarerequiredtoincreasethesensitivityandspecificityofserumscreeningforearlyHCCdiagnosis.ThisstudyemployedaquantitativeproteomicstrategytoanalyzethedifferentialexpressionofserumglycoproteinsbetweenHCCandnormalcontrolserumsamples.Methods:Lectinaffinitychromatography(LAC)wasusedtoenrichglycoproteinsfromtheserumsamples.Quantitativemassspectrometricanalysiscombinedwithstableisotopedimethyllabelingand2Dliquidchromatography(LC)separationswereperformedtoexaminethedifferentiallevelsofthedetectedproteinsbetweenHCCandcontrolserumsamples.Westernblotwasusedtoanalyzethedifferentialexpressionlevelsofthethreeserumproteins.Results:Atotalof2,280proteingroupswereidentifiedintheserumsamplesfromHCCpatientsbyusingthe2DLC-MS/MSmethod.Upto36proteinswereup-regulatedintheHCCserum,whereas19proteinsweredown-regulated.Threedifferentialglycoproteins,namely,fibrinogengammachain(FGG),FOS-likeantigen2(FOSL2),andα-1,6-mannosylglycoprotein6-β-N-acetylglucosaminyltransferaseB(MGAT5B)werevalidatedbyWesternblot.Allthesethreeproteinswereup-regulatedintheHCCserumsamples.Conclusion:AquantitativeglycoproteomicmethodwasestablishedandprovenusefultodeterminepotentialnovelbiomarkersforHCC.

简介：Afterprimaryanalysesontheserumglycocon-jugatesoflungcancerandnormalindividulusingtheenzyme-linkedlectinassay(ELLA)with12kindsoflectins,PHAandLCAwereselectedtofurtherstudyintheseraof8kindsofcancers,4kindsofnon-malignantdiseaseand1kindofpostoperativecancer.Itwasfoundthatthetestvaluesof7kindsofcancerswithPHAorLCAweresignificantlyhigherthanthatofthenormal(P<0.01);thevaluesof4kindsofnon-malignantdiseaseswithPHAwerenothigher(P>0.05);thevaluesofthepostoperativecancerwithPHAwereobviouslylowerthanthatofthepreoperative(P<0.05).TheresultsshowedthattheserumglycoconjugateswhichcanbindtoPHAseemedrelatedtothecancerousexistenceinhumanbodies.Thesignificanceofthefindingswasdiscussed.

简介：Objective:MicroRNA-21(miR-21)hasbeenshowntobeakeyregulatorofcarcinogenesis.TherewerefewreportsaboutthecomparisonofserummiR-21withconventionaltumormarkers.ThisstudyaimedtoexplorethediagnosticvalueofcirculatingmiR-21asatumormarkerinbreastcancer(BC)andcompareitwithCA153andcarcinoembryonicantigen(CEA).Methods:CirculatingmiR-16andmiR-21wereamplifiedandquantitativelydetectedbyreal-timePCRin89BCpatientsand55healthycontrols.ThelevelsofCA153andCEAweremeasuredthroughelectrochemiluminescenceassays.ThenthesensitivityindiagnosisofBCwascomparedamongmiR-21,CA153andCEA.Results:ThelevelofserummiR-21wassignificantlyhigherinBCpatientsthancontrols(P<0.001).ThesensitivityandspecificityofmiR-21were87.6%and87.3%,respectively,whereasthesensitivitiesofCEAandCA153wereonly22.47%and15.73%.Conclusions:ComparedwithCEAandCA153,serummiR-21hasahighersensitivityindiagnosisofBC.AlthoughnotcorrelatedwiththestatusofER,PRandclinicalstages,serummiR-21maybeapotentialdiagnosticindicatorforBC,especiallyfortheearlystage.

简介：TheeffectofTPA,apotenttumorpromoter,onSSV-NIH3T3cellsinserum-freemediumwasinvestigated.TPAstimulatedDNAsynthesisofSSV-NIH3T3cellsonthethirddayofcultureinSFM.InSDS-PAGFofmediumconditionedbyTPA-treatedSSV-NIH3T3cells(inSFM+TPA),theamountsoffourproteinsof31.0Kd,28.5Kd,25.5Kdand13.5Kdstrikinglyincreasedoverthatofnon-TPA-treatedcounterpart(inSFM).ThePDGF-likeactivitywasalsodetectedinCMofSFM+TPA.WheninsulinandEGFweredrownofftheSFM+TPA(SFM-Ins-EGF+TPA),TPAlostitsabilitytostimulateDNAsynthesisofSSV-NIH3T3cellsonthethirddayandSDS-PAGEoftheconditionedmediumshowedthattheamountsofthefourproteinsnotedabovegratelyreduced.However,cellsinSFM-Ins-EGF+TPAwereinalmostthesamegrowthconditionascellsincompleteSFM+TPAonthethirddayofculture.Resultswerediscussedinthepaper.