简介:Inthepresentstudy,theeffectofblockageofthecostimulatorysignalCD86attimeofimplantationontheexpressionsofTGF-β1,MMP-9,TIMP-3andPAI-1proteinsatthematernal-fetalinterfaceandtheoutcomeofpregnancyinmurineabortion-pronemodelwasinvestigated,inwhichtheCBA/JxDBA/2matingswereusedastheabortion-pronemodelandtheCBA/J×BALB/cmatingsusedasthenormalpregnantmodel.Thestudywasperformedinfollowingthreegroups:2groupsoftheabortion-pronemodel,whichwereexperimentalgroupandcontrolexperimentalgroup,and1groupofnormalpregnantmodel,andeachgrouphad10pregnantCBA/Jmiceexclusively.FemalepregnantCBA/Jmiceintheexperimentalgroupreceivedanintraperitoneal(i.p.)injectionof100μgofantimouseCD86mAbin200μlofPBSatday4.5ofgestation,andtheirrelevant-isotopematchedratIgG2bwasadministratedinthecontrolexperimentalgroupwiththesamedosageandatsametime.Forthenormalpregnantgroup,notreatmentwasgiven.ThepregnantCBA/Jmicewerekilledonday13.5ofgestation.Then,theembryoresorptionratewascalculatedandtheexpressionsofTGF-β1,MMP-9,TIMP3andPAI-1weredetectedbyusingimmunohistochemicalmethods.Itwasdemonstratedthattheembryoresorptionrateintheexperimentalgroupwassignificantlyreducedincomparisonwiththatinthecontrolexperimentalgroup(x2=7.441,P=0.006),buttherewasnosignificantdifferencewiththatinnormalpregnantgroup(x2=0.016,P=0.898).TheexpressionsofTGF-β1andPAI-1intheexperimentalgroupweresignificantlyincreasedincomparisonwiththatinthecontrolexperimentalgroup(P=0.010,P=0.003,respectively),withnosignificantdifferencefromthatinthenormalpregnantgroup(P=0.500).However,theexpressionofMMP-9intheexperimentalgroupwassignificantlyreducedincomparisonwiththatinthecontrolexperimentalgroup(P=0.012)withnosignificantdifferencefromthatinthenormalpregnantgroup(P=0.500).Theexpression
简介:ToscreenandidentifytheshortpeptideswithspecificbindingactivitytohumanCDS9andtodesigntheshort-peptideclampagainsttumorescape,thephagedisplaypeptidelibrarycontaining12peptideswasusedtoselectthehighlyexpressedspecificcoalescentpeptideofhumanCD59inCHOcells.Positivephageclonesobtainedafter5roundsofbiopanninganddetectedwithELISAwereobtained,inwhich8ofthemwithhighbindingactivitytohumanCD59weresequenced.The3sequencesthusobtainedshowedhighhomologywitheachandcertainhomologywithsequencewithhumanCD2(PubMed339HGAAENSISPSS),andallcontainedprimarystructureHXAXXXXXXPXX,ofwhichthissequencemaybethemimicconformationalepitopebindingtohumanCD59.Theseresultsinthepresentstudymaybehelpfultodesigntheshort-peptideclampagainsttheactivesitesofCD59ontumorescape.
简介:ToexploretheeffectofrhlL-15onCB-CD34^+stemcellscommittingtoNKcells,CD34^+stemcellswereobtainedfromcordblood(CB)bymagnetic-assistedcellsorting(MACS)method.CD3,CD16andCD56moleculesexpressedoncellsurfaceweredetectedbyflowcytometer.MTFmethodwasusedtotestthecytotoxicityofNKcells.Theresultswerethatstemcellfactor(SCF)alonehasnoeffectonCD34^+stemcells.IL-15stimulatedCD34^+stemcellscommittoNKcells,andSCFshowedstrongsynergisticeffectwithIL-15.ItwasconcludedthatIL-15andSCFplayeddifferentrolesduringNKcelldevelopment,llr15promotedCD34^+stemcellsdifferentiatetoNKcellprecursorandSCFimprovedtheeffectsofIL-15onNKcelldifferentiation.
简介:Twenty-oneyearsaftermalariaantigenswerefirstclonedavaccinestillappearstobealongwayoff.Therehavebeenperiodsofgreatexcitementandinmodelsystemssubunitvaccinehomologuescaninducerobustprotection.However,significantchallengesexistconcerningantigenicvariationandpolymorphism,immunologicalnon-respons-ivenesstoindividualvaccineantigens,parasite-inducedapoptosisofimmuneeffectorandmemorycellsandimmunedeviationasaresultofmaternalimmtmityandalterationsofdendriticcellfunction.
简介:TostudythemechanismofinfectionofEpstein-Barrvirus(EBV)ingastriccarcinomacells,theAkataandP3HR-1strainsofEBVwereusedastheteststrainsofviruses,andthesignetringcelllineHSC-39ofgastriccarcinomacellswasusedasthetargetcellsofinfection.Thevirus-infectedcellcloneswereisolatedbylimiteddilutionmethod.ItwasfoundthattheEBV-encodedsmallRNA(EBER)couldbedetectedintheinfectedcells.TheAkataandP3HR-1EBVinfectedparentalcellsandmostofclonesexpressedEBNA1,butnotEBNA2.Latentmembraneprotein(LMP-1)andLMP-2,andtheQpromoter(p),butnottheCp/WpforEBNAgenetranscriptionwasactiveintheinfectedparentalcellsaswellasalltheclones.UninfectedHSC-39cellsdidnotexpressCD21,however,AkatabutnotP3HR-1EBV-infectedclonesex-pressedlowlevelofCD21mRNA.TheseresultsdemonstratethatHSC-39cellsaresusceptibletobothEBVstrainsandEBVinfectsHSC-39cellsthroughtheCD21-independentpathway.ThisstudydefinesasignetringtypeofgastriccarcinomacellslineasauniquetargetcellsforthestudyofEBVinfectionmechanism.
简介:ExposureofnaivemurineCD4^+TlymphocytestosuperantigensuchasstaphylococcalenterotoxinB(SEB)inducesastrongproliferativeresponse.ProlongedexposureorsubsequentrestimulationoftherespondingTcellpopulationwithSEBleadstotheapoptoticeventsofactivation-inducedcelldeath(AICD).ThesignalingmechanismresponsiblefortheAICDisatargetofintensiveinvestigation.However,theprecisedownstreamsignahngpathwaysofSEB-inducedAICDremainsunclear.Ourresultshereshowthatthesequentialactivationofcaspase-1/ICE-hkeandcaspase-3/CPP32-hkecysteineproteasesprobablyplaysaroleinthesignalingtransductionofSEB-inducedAICD,butcaspase-3/CPP32-hkeproteasesactivationdoesnotdependoncaspase-1-likeproteasesactivation.HerbimycinA,aspecificinhibitorofproteintyresinekinases,inhibitcaspase-3/CPP32-1ikecysteineproteasesactivation.However,itdoesnotpreventDNAfragmentationofCD4^+TcellsapoptosisinducedbySEB.TheseresultsindicatethatproteintyrosinekinasespathwayisprobablyinvolvedinthesignalingtransductionofCD4^+TcellsapoptosisinducedbySEBand“crosstalks”withthepathwayofcaspase-3/CPP32-1ikeproteasesactivation.
简介:摘要:目的:研究全身麻醉术后低体温的复苏期护理疗效。方法:采用科学数字随机法对本院 2018年 1月到 2019年 1月收治行全身麻醉患者 86例分为 L组和 C组,每组 43例。 C组行全麻术后常规复苏期护理, L组行全麻术后低体温护理干预,比较 L组与 C组干预后低体温的发生率及平均血氧饱和度、血氧饱和下降发生率。结果: L组术后低体温的发生率为 6.98%( 3/43)明显低于 C组低体温发生率 27.91%( 12/43),且 L组术后平均血氧饱和度明显优于 C组( P< 0.05); L组术后血氧饱和下降的发生率为 23.26%( 10/43)明显低于 C组血氧饱和下降的发生率 55.81%( 24/43)比较差异有统计学意义( P< 0.05)。结论:全身麻醉术后进行低体温护理干预可有效降低低体温发生率和血氧饱和下降发生率,提高血氧饱和度。
简介:【摘要】 目的: 提高肝硬化大量腹水患者的护理质量。 方法: 回顾分析 2018 年 2 月至 2019 年 2 月医院收治的 80 例肝硬化大量腹水患者,按随机抽签法将其分为实验组和常规组,每组 40 例。给予常规组肝硬化腹水基础护理,实验组综合护理,比较两组患者的治疗效果。 结果: 两组患者的治疗效果对比,实验组的总有效率明显优于常规组的总有效率,护理效果显著,差异具有统计学意义( P<0.05 )。 结论: 综合护理在 80 例肝硬化腹水患者治疗中效果更为明显,患者的病情有所缓解,并发症发生率在护理下减少,降低患者的心理压力和经济压力,改善患者生活质量,是一种较为可靠的护理模式,值得在临床医学上推广和运用。
简介:【摘要】目的:探究80例剖宫产后再次妊娠经阴道分娩产妇的护理。方法:对2019年5月-2020年5月我院的剖宫产后再次妊娠经阴道分娩产妇80例进行调查研究,根据产妇到院顺序进行编号,将编号为单数的产妇分为对照组,编号为双数的产妇分为观察组,两组产妇采用不同的护理措施。护理后,对比两组产妇分娩时出血量、产程时间以及并发症发生情况。结果:护理后,观察组产妇分娩时出血量、产程时间以及并发症发生率,均显著低于对照组产妇各研究指标,数据差异具有统计学意义,P<0.05。结论:对于剖宫产后再次妊娠经阴道分娩产妇进行全程护理干预具有重要意义,能有效的减少产妇分娩时出血量,降低产妇并发症发生率,值得推广。
简介:【摘要】目的: 探讨神经电刺激对 ICU 获得性衰弱的预防作用。 方法: 随机抽取我院 2017 年 10 月 -2019 年 10 月诊疗的 52 例重症慢性阻塞性肺疾病患者,划分为 A 组( 26 例)和 B 组( 26 例)。即 A 组为传统护理和治疗, B 组在 A 组的基础上使用神经电刺激疗法,对比患者谵妄总发生率、机械通气时间。 结果: A 组谵妄总发生率为 34.62% 、机械通气时间为( 12.4±5.27 ) h , B 组为 7.69% 、( 6.9±4.19 ) h ,数据间比较有意义( P < 0.05 )。 结论: 于重症慢性阻塞性肺疾病患者中,有效且科学的神经电刺激疗法,能够有效降低谵妄发生率,还可缩短机械通气时间,应引起重视。
简介:【摘要】目的:针对当前医院感染管理与护理管理的协同效果开展深入分析。方法:选择了我院2021年1月到2021年12月护理的康复患者为研究对象,随机数字法将220例研究对象分两组。研究组(n=110例)给予医院感染管理与护理管理的协同管理;参照组(n=110例)给予常规管理。结果:参照组的不良反应高于研究组,组间差异具有统计学意义(P