简介:Lipoxygenase3(LOX3)isamajorcomponentoftheLOXisozymesinmaturericeseeds.ToinvestigatetheroleofLOX3geneunderstresses,aplantexpressionvectorcontainingantisensecDNAofLOX3wasconstructed.RicevarietiesWuyunjing7andKasalathweretransformedbytheAgrobacterium-mediatedmethodandtransgenicriceplantsweregenerated.PCRandSouthernblotresultsshowedthattheantisenseLOX3genewasintegratedintothericegenome.AnalysesofembryoLOX3deletionandsemi-quantitativeRT-PCRconfirmedtheantisensesuppressionofLOX3geneintransgenicplants.TheT2antisenseplantsofLOX3weresensitivetodroughtstress,riceblastandbacterialblightcomparedwithnon-transgenicplants.TheseresultssuggestthattheLOX3genemightfunctioninresponsetostresses.
简介:FieldlysimetermethodwasemployedtoinvestigatethedownwardmovementandleachingofNappliedtosummercorn(ZeamayL.)ondrylandsoilinBeijing,AN-fertilizer(120kgNhm^-2)andacontroltreatmentwerarrangedforthestudy.Soilsolutionwascollectedatdepthsof20,40,60,120and170cm,whileleachtewasollectedatthebottom(220cm)ofthelysimeter,TheresultsshowedthatthedownwardmovementofNO3^-Ninsoilprofilewasgreatlyaffectedbyrainfallpattern.ThepeakofleachedNO3^-Nfrombothtreatmentscoincidedwiththepeakoftherainfall.Inaddition,leachedNO3^-Nfrombothtreatmentsandrainfallweresignificantlycorrelated(P<0.05).TheamountofleachedNO3^-NwasnotgreatintheN-fertilizertreatment,TheresultsalsosuggestedthatNfertilizationcouldcauseNO3^--Ncontaminationofgroundeaterduringtherainyseason.
简介:对来自中国、俄罗斯和美国的北极七鳃鳗L.camtschaticumCOI部分序列82条、俄罗斯的溪七鳃鳗L.reissneri17条和中国的朝鲜七鳃鳗L.morii3条,共计102条进行亲缘关系和系统进化分析,以明确七鳃鳗属Lethenteron3个种类的分类地位和亲缘关系。单倍型检测发现,北极七鳃鳗共有14种单倍型,其中中国群体4种,俄罗斯群体11种,美国群体3种,大部分个体共享2种单倍型;溪七鳃鳗有3种单倍型,均与北极七鳃鳗共享;朝鲜七鳃鳗辽宁丹东群体和黑龙江抚远群体各独享1种单倍型。系统进化分析显示,除了朝鲜七鳃鳗黑龙江群体Hap16单独聚类外,其余15种单倍型无明显分化,聚为一支。单倍型序列分歧检测发现,黑龙江群体Hap16无论种内或种间,均与其他15种单倍型分歧达到种级(~2%)分化水平。因此,推测朝鲜七鳃鳗黑龙江群体为七鳃鳗属一个有效种,溪七鳃鳗为北极七鳃鳗的不同生态类型。