简介:目的:探讨糖尿病视网膜病变(diabeticretinopathy,DR)患者血清中生长停滞特异基因6(growtharrestspecificgene6,Gas6)、基质细胞衍生因子(stromalcell-derivedfactor-1,SDF-1)和SDF-1β表达的变化及临床意义。方法:DR患者113例分为增殖性组(n=45)和非增殖性组(n=68),同期选取未合并DR糖尿病患者作为糖尿病组(49例)和健康者42例,检测血糖、血脂和超敏C-反应蛋白(highlysensitiveC-reactiveprotein,hs-CRP)指标,利用实时荧光定量PCR技术检测血清中Gas6、SDF-1α和SDF-1β基因表达。结果:糖尿病组、非增殖性组和增殖性组患者稳态模型的胰岛素抵抗指数(homeostasismodelassessmentforinsulinresistance,HOMA-IR)、甘油三酯(triglycerides,TG)和hs-CRP均逐渐升高,差异均有统计学意义(F=39.672、81.625、99.872,均P〈0.05),增殖性组和非增殖性组患者低密度脂蛋白(low-densitylipoprotein,LDL-C)均高于糖尿病组和健康者,而高密度脂蛋白(highdensitylipoprotein,HDL-C)均低于糖尿病组和健康者,差异均有统计学意义(F=51.974、43.824,均P〈0.05);增殖性组、非增殖性组和糖尿病组患者血清Gas6mRNA表达量均高于健康者,增殖性组患者血清SDF-1αmRNA表达量均高于非增殖性组、糖尿病组和健康者,增殖性组和非增殖性组患者血清SDF-1βmRNA表达量均高于糖尿病组和健康者,且增殖性组患者高于非增殖组,差异均有统计学意义(F=15.381、21.589、38.942,P〈0.05);Pearson相关分析显示,DR患者血清Gas6mRNA表达量与TG、总胆固醇(totalcholesterol,TC)和LDL-C呈正相关(r=0.228、0.241、0.209,均P〈0.05),血清SDF-1αmRNA和SDF-1βmRNA表达量均与hs-CRP呈正相关(r=0.297、0.325,P〈0.05)。结论:DR患者血清中Gas6、SDF-1α和SDF-1β基因表达水平升高,可能与患者血脂代谢异常及炎性反应有关。
简介:目的:研究高氧诱导的视网膜新生血管模型鼠中转录因子Islet-1的表达差异。方法:采用高氧诱导的方法制作鼠视网膜新生血管模型,运用荧光造影视网膜铺片及视网膜切片苏木精-伊红染色观察视网膜新生血管的形态。于小鼠出生后第7,12,14,17,26d取视网膜组织,采用Real-timePCR及Westernblot技术测定视网膜组织中Islet-1的表达水平。结果:模型组视网膜铺片及组织切片可见大量视网膜新生血管形成。小鼠出生后第7d,模型组与正常组视网膜组织中Islet-1表达水平无明显差异;小鼠出生后第12~14d,模型组视网膜组织中Islet-1表达水平明显上调;出生后17d,模型组视网膜组织中Islet-1表达水平仍高于正常组;出生后26d,随着视网膜新生血管消退,视网膜组织中Islet-1表达水平降至正常水平。结论:模型鼠视网膜新生血管发生过程中,持续缺氧的视网膜组织通过增加转录因子Islet-1的表达,从而诱导视网膜新生血管的发生。
简介:AIM:TocomprehensivelyevaluatethepotentialassociationofCOL1A1polymorphismswithhighmyopiabyasystematicreviewandMeta-analysis.METHODS:AllassociationstudiesonCOL1A1andhighmyopiareporteduptoJune10,2014inPubMed,Embase,WebofScience,andtheChineseBiomedicalDatabasewereretrieved.Oddsratios(ORs)and95%confidenceintervals(95%CIs)wereanalyzedforsinglenucleotidepolymorphisms(SNPs)usingfixed-andrandom-effectsmodelsaccordingtobetween-studyheterogeneity.PublicationbiasanalyseswereconductedbyEgger’stest.RESULTS:Atotaloffourstudiesfromreportedpaperswereincludedinthisanalysis.TheMeta-analysesforCOL1A1rs2075555,composedof2304highmyopiapatientsand2272controls,failedtodetectanysignificantassociationwithhighmyopia.Atotalof971casesand649controlsweretestedforCOL1A1rs2269336.TheassociationofCOL1A1rs2269336withhighmyopiawasobservedinrecessivemodel(CCvsCG+GG,P=0.03)andinheterozygousmodel(CGvsGG,P=0.04),butnotinothermodels.CONCLUSION:ThisMeta-analysisshowsthatCOL1A1rs2269336(CCvsCG+GG)affectsindividualsusceptibilitytohighmyopia,whereasthereisnoassociationdetectedbetweenSNPsrs2075555andhighmyopia.Giventhelimitedsamplesize,furtherinvestigationsincludingmoreethnicgroupsarerequiredtovalidatetheassociation.
简介:目的:通过分析细胞因子的性质,旨在探讨前葡萄膜炎可能的发病机制。方法:取20例HLA-B27相关前葡萄膜炎患者、20例小柳原田患者外周血和正常人作为对照组,通过酶联免疫反应(ELISA)检测患者外周血IFN-γ,IL-4和IL-10的含量。结果:两组患者外周血IFN-γ的含量较正常人显著增高(P〈0.05),且小柳原田患者外周血中IFN-γ含量显著高于HLA-B27相关前葡萄膜炎患者外周血中IFN-γ含量(P〈0.05)。结论:前葡萄膜炎发病机制可能和对自身抗原诱发的Th1型免疫反应过强有关。