简介：Wereportonthedesign,realization,andoutputperformanceofadiode-pumpedhigh-peak-powerpassivelyQ-switchedNd:YAG∕Cr4+:YAGcompositemediummonolithiclaserwithfour-beamoutput.Theenergyofalaserpulsewashigherthan3mJwithdurationof0.9ns.Theproposedsystemhastheabilitytochooseindependentlythefocusofeachbeam.Suchalaserdevicecanbeusedformultipointignitionofanautomobilegasolineengine,butcouldalsobeofinterestforignitioninspacepropulsionorinturbulentconditionsspecifictoaeronautics.

简介：BACKGROUND:Culturesfrommultipleportionsofumbilicalcordbloodmesenchymalstemcellshavebeenshowntoundergomorerapidproliferationandattachmentthansingleportions.OBJECTIVE:Toobservegrowthofbasicfibroblastgrowthfactor(bFGF)-inducedculturesofhumanamnion-derivedmesenchymalstemcells(AMSCs)anddifferentiationintoneuronal-likecells.DESIGN,TIMEANDSETTING:Comparativeobservation.ThestudywasperformedattheLaboratoryofMicrobiologyandImmunology,BasicMedicalSchoolofZhengzhouUniversityfromJanuarytoMay2008.METHODS:Amniafromfull-term,uterine-incisiondeliveryweredonatedby12healthywomen.AMSCswereobtainedbycellseparationandculturetechniques,andwerepassagedandinducedbybFGF.Fromthethirdpassage,atotalof1mLAMSCs,atadensityof1.0×10~4/mL,wasseparatelyharvestedfromsixsamples,whichservedasgroupA.Atotalof1mLAMSCs,atadensityof1.0×10~4/mL,washarvestedseparatelyfromtheremainingsixsamples,whichservedasgroupB.Atotalof0.5mLfromthesixsamplesofgroupAand0.5mLfromthesixsamplesofgroupBwerecombinedtoformgroupC.MAINOUTCOMEMEASURES:Differencesincellquantityamongthethreegroupswerecomparedbycellquantificationand3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)analysis.Expressionofaglialcellmarker,neuron-specificenolase,andnestinwasdetectedinthethreegroupsbyimmunocytochemistry.RESULTS:CellquantificationandMTTanalysisoflivecells,aswellasAMSCabsorbance,weresignificantlygreateringroupCcomparedwithgroupsAandBat18daysofculture(P<0.05),andnosignificantdifferencewasobservedbetweengroupsAandB.Glialfibrillaryacidicprotein,neuron-specificenolase,andnestinwereexpressedinallgroupsfollowingbFGFinduction.CONCLUSION:MixedAMSCculturespromotedproliferation,andbFGF-inducedAMSCsdifferentiatedintoneuronal-likecells.

简介：AbstractObjective:The coronavirus disease 2019 (COVID-19) epidemic resulting from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has still spread globally. The occurrence of the Delta variant, which is more infectious and spreads faster than earlier forms of the virus that causes COVID-19, makes infection prevention more challenging. Therefore, this study aimed to gain a comprehensive insight into the transmission routes of SARS-CoV-2 for curbing the propagation of SARS-CoV-2 in human populations.Methods:We studied a prospective cohort of 576 patients admitted consecutively to the First Affiliated Hospital of Guangzhou Medical University from January 21 to June 8, 2020. These patients were chosen based on their similar clinical phenotypes or imaging findings. There were 21 (3.6%) laboratory-confirmed COVID-19 patients (16 severe and 5 mild cases) and 555 non-COVID-19 patients. The antibody response and routes and duration of viral shedding were systematically evaluated in serial clinical specimens. Moreover, SARS-CoV-2 RNA was also detected in a mouth rinse, urine, and tear samples. This study was approved by the Medical Ethical Committee of The First Affiliated Hospital of Guangzhou Medical University (approval No. 2020-77).Results:SARS-CoV-2 mainly existed in sputum, nasal and throat swabs, and feces samples. Virus latency was longer in sputum and feces samples than in nasopharyngeal samples. IgG antibody response in respiratory samples was related to disease severity. Although droplets and aerosols are the major transmission routes for COVID-19, covert routes of transmission from asymptomatic patients, contaminated surfaces, and wastewater are also of interest.Conclusion:Our findings provide a solid foundation for developing prophylactic measures against SARS-CoV-2.

简介：Consideringthegreatpotentialofnaturalproductsasanticanceragents,thepresentstudywasdesignedtoexplorethemolecularmechanismsresponsibleforanticanceractivitiesofMesuaferreastembarkextractagainsthumancolorectalcarcinoma.BasedonMTTassayresults,bioactivesub-fraction(SF-3)wasselectedforfurtherstudiesusingHCT116cells.Repeatedcolumnchromatographyresultedinisolationoflessactiveα-amyrinfromSF-3,whichwasidentifiedandcharacterizedbyGC-MSandHPLCmethods.α-amyrinandbetulinicacidcontentsofSF-3weremeasuredbyHPLCmethods.Fluorescentassaysrevealedcharacteristicapoptoticfeatures,includingcellshrinkage,nuclearcondensation,andmarkeddecreaseinmitochondrialmembranepotentialinSF-3treatedcells.Inaddition,increasedlevelsofcaspases-9and-3/7levelswerealsoobservedinSF-3treatedcells.SF-3showedpromisingantimetastaticpropertiesinmultipleinvitroassays.Multi-pathwayanalysisrevealedsignificantdown-regulationofWNT,HIF-1α,andEGFRwithsimultaneousup-regulationofp53,Myc/Max,andTGF-βsignallingpathwaysinSF-3treatedcells.Inaddition,promisinggrowthinhibitoryeffectswereobservedinSF-3treatedHCT116tumourspheroids,whichgiveahintaboutinvivoantitumorefficacyofSF-3phytoconstituents.Inconclusion,theseresultsdemonstratedthatanticancereffectsofSF-3towardscoloncancerarethroughmodulationofmultiplemolecularpathways.

简介：

简介：The1.3-dipolarcycloadditionreactionof2-trifluoromethyl-oxazoloneandtheactivatedcarbon-carbonmultiplebondwasstudiedandgaveaconvenientwaytosynthesize2-trifluoromethylpyrrolederivatives.

简介：作为在microsurgeries的最挑战性的过程，Vasoepididymostomy(VE)经常与双armed被执行二缝术的技术。在这研究，我们在人上评估了武装单人赛的二缝术的VE的功效和安全并且学习了能可能影响明显率的因素。从2012年7月到2013年7月，我们与连续主要epididymal阻塞考察了81个病人由一位单身的外科医生，经历了武装单人赛的二缝术的纵的摄取microsurgicalVEKaihong(KH)。同时，我们分析了可能与明显有关评价的七个因素。与武装单人赛的技术，81个人的一个总数经历了microsurgicalVE。62个病人上的数据完全被记录。19个病人被失去到后续。吝啬的年龄是31岁。吝啬的后续时间是8.8(2-17)月。明显率是66.1%(41/62)。自然怀孕率是34.1%(14/41)。全面怀孕率是22.6%(14/62)。没有严重外科的复杂并发症被注意。与逻辑回归测试分析，与更高的明显率有关有二个因素：吻合地点(P=0.035)并且能动精子在epididymal液体发现了(P=0.006)。在epididymal液体发现的能动精子与更高的明显率被联系(或=11.80，95%CI=1.79，77.65)。武装单人赛的二缝术的纵的VE技术为microsurgical实践是可行的。明显和怀孕率比得上双手臂的技术。在epididymal液体发现的吻合地点和能动精子是与更高的明显有关的大多数二个重要因素。

简介：

简介：Organicmultiplequantumwells(OMQWs)consistingofalternatinglayersoforganicmaterialshavebeenfabricatedfromtris(8-hdroxyquinoline)aluminum(Alq)and2-(4-biphenylyl)-5-(4-tertbutylphenyl)-1,3,3-oxadiazole(PBD)byamultisource-typehigh-vacuumorganicmoleculardeposition.Fromthesmall-angleX-raydiffractionpatternsofAlq/PBDOMQWs,aperiodicallylayeredstructureisconfirmedthroughtheentirestack.TheAlqlayerthicknessintheOMQWswasvariedfrom1nmto4nm.Fromtheopticalaborption,photoluminescenceandelectroluminescencemeasurements,itisfoundthattheexcitonenergyshiftstohigherenergywithdecreasingAlqlayerthickness,ThechangesoftheexcitonenergycouldbeinterpretedastheconfinementeffectsofexcitonintheAlqthinlayers.Narrowingoftheemissionspectrumhasalsobeenobservedfortheelectroluminescentdevices(ELDs)withtheOMQWsstructureatroomtemperature.

简介：TocharacterizeandidentifymultipleconstituentsinDanhonginjection(DHI),afastultra-highperformanceliquidchromatographycoupledtoelectrosprayionizationquadrupoletime-of-flighttandemmassspectrometry(UHPLC-ESI-QTOF/MS)methodwasestablishedandvalidatedinthepresentstudy.Atotalof63compounds,including33phenolicacids,2C-glycosylquinochalcones,6flavonoidO-glycosides,4iridoidglycosides,6organicacids,5aminoacids,and3nucleosides,wereidentifiedortentativelycharacterized.Inconclusion,theUHPLC-ESI-QTOF/MSmethodisusefulandefficientforin-depthstructuralelucidationofchemicalcompoundsincomplexmatricesofherbalmedicinessuchasDHI.

简介：目的将在细胞生长，细胞周期和p21wap1/cip1和p27kip1的表情上调查triptolide(TPL)的效果。方法MTT试金被用来在人的多重骨髓瘤RPMI-8226房间在triptolide处理以后决定房间生存能力。房间周期分发上的效果被流动cytometry决定。半量的反向的transcription-PCR被用来检验p21wap1/cip1和p27kip1的mRNA表情。p21wap1/cip1和p27kip1的蛋白质表情被西方的污点决定。改变集中的结果Triptolide以剂量相关、时间相关的时尚导致了房间生存能力抑制并且在RPMI-8226房间引起了G0-G1房间周期前进的阶段拘捕。伴有p21wap1/cip1和p27kip1的表情的起来调整的这些效果。这些结果建议那triptolide的结论经由起来调整的p21wap1/cip1和p27kip1禁止房间增长和房间周期前进，triptolide可以通过这条小径施加它的反癌症活动。

简介：抗原特定的CD8T房间的显型和功能仔细与治疗学的种痘的功效被联系。这里，我们证明有在Freund的助手的LCMVgp33-41肽(KAV)的多重免疫能与CD127和CD62L分子的低表示导致KAV特定的CD8T房间。禁止的受体PD-1也在怒气和淋巴节点在KAV特定的CD8T房间上在KAV特定的CD8T房间，和它的表示水平的实质的部分上被表示高得多什么时候与那些相比在外部血。而且，KAV特定的CD8T房间能明确地在vivo杀死搏动KAV的目标房间，但是效率是低的。这些数据建议有在Freund的助手的肽的那张主要增加的种痘时间表能与部分功能的疲劳得到像受动器的显型的抗原特定的CD8T房间，它可以仅仅对病原体提供短期的保护。