简介：BackgroundAnimalmodelsofmyocardialinfarction(MI)havebeenwidelyusedtostudythepathologicalandphysiologicalchangesthatoccurinMI,andtoobjectivelyevaluatetheefficacyofnewtreatments.Theyareanimportanttoolinthisprocedure.However,themortalityrateofMIanimalmodelshassofarbeenhigherthaninreal-lifesituations.Theaimofthisstudywastoexploretheuseofamodifiedretrogradetractiontrachealintubation(MRTI)methodforincreasingthesuccessrateofMImodelsinrats.MethodsSixtymaleSprague-Dawleyratswereusedintheexperiment.UsingtheMRTImethodofartificialairwaygeneration,weestablishedtheMImodelbyligationoftheleftanteriordescendingbranchofthecoronaryartery.WeanalyzedtheeffectsofMRTI,theuseoflidocaine,operativedetails,nursingconsiderationsduringtheoperation,andpost-operativefactorsonthesuccessrateoftheMImodelinrats.ResultsThesuccessrateofgeneratinganMImodelinratscanbesignificantlyincreasedusingthefollowingmethods:1)SettinguptheartificialairwaythroughtheuseofMRTIbyusingasingle-lumencentralvenouscatheter;2)Selectingaligationsite2mmbelowthemidpointoftheconnectionbetweentheleftatrialappendageandthepulmonarycone;3)Addingadropoflidocainetothesurfaceofthehearttoslowdowntheheartrate,maketheoperationeasiertoperform,andpreventarrhythmiaspostoperatively;4)Clearingupairwaysecretionstimelybothintraandpostoperatively;5)Makingsurethatratsareinawarmstatebothintraandpostoperatively;6)Preventingwoundinfection.ConclusionsUseoftheMRTImethodcanquicklyestablishanartificialairwayinrats.Intraoperativeuseoflidocaine,selectingaprecisevascularligationsite,andappropriatecarebothintraandpostoperativelycanincreasethesuccessrateofMImodelgeneration.更多还原