简介：Objective:ToinvestigateU.parvum(previouslyUreaplasmaurealyticumbiovar1)andU.urealyticum(previouslyUreaplasmaurealyticumbiovar2)andtheirsubtypesandserovarsinurinespecimensofpregnantwomen.Methods:Aftercollecting151specimensandinoculatingbroth,allbrothculturepositive(ureasepositive)specimenswereamplified,specieswereidentifiedandsubtypedbyusinggeneralprimers,species-specific,andtype-specificprimerstargetingthemultiplebandedantigen(MBA)genesequence.Results:U.parvumwasidentifiedin58of151specimensandU.urealyticumin18(bothwerepresentin5,andneitherwerefoundin6).Serovars3,1,and6werethemostcommonamongU.parvumisolatesandsubtypes1and3werethemostcommonamongU.urealyticum.Multipleserovarsamongclinicalisolateswerefound.Conclusion:ThisPCR-basedtypingsystemcouldfacilitatestudiesoftherelationshipbetweenindividualureaplasmaspeciesorsubtypesandhumandiseases.

简介：Objective:Toanalyzesubtypesandquasi-speciesofisolatedvirusesfromHIV-1infectedindividualsamongthepopulationepidemiologicaldynamicsoflocalHIV-1isolates,thuslayingafoundationfordesigningacandidateAIDSvaccine.Methods:Byhetero-duplexmobilityassay(HMA)andsinglestrandconformationpoly-morphism(SSCP)analysisonampliconsfromsingle-primedpolymerasechainreaction(SP-PCR),subtypesandquasi-speciesoftestedHIV-1isolateswereelucidated,andampliconsweresequencedforconfirmation.Results:Specificampliconsfromdifferentsubtypesandquasi-speciesofHIV-1couldbediscerniblebyHMAandSSCPanalysis.HIV-1isolatesfromdifferentpatientsmightbeeitheradifferentsrbtypeoranidenticalsubtype,andHIV-1isolatesfromanindividualwerepresentinapopulationofquasi-species.