简介：AIMTo调查在patients.METHODSNon-DSEK在接枝拒绝率，和它的全面程序的有效性上剥去endothelialkeratoplasty(non-DSEK)的non-Descemet的影响在64个病人，和程序的结果的65只眼睛上被执行包括接枝的拒绝事件，失败和脱臼，最好改正的视觉尖酸(BCVA)，endothelial房间密度(ECD)，和另外的复杂并发症，是65看的分析retrospectively.RESULTSOf，63从bullouskeratopathywi恢复了吝啬的后续时间是26.4mo(范围，6-84mo)。吝啬的BCVA在外科以后在1y在3mo，在6mo的0.46logMAR，和0.37logMAR外科手术前地从1.70logMAR改善了到0.54logMAR。成功地经历了镜子的显微镜的检查的25个病人的手术后的施主ECD是1918<

简介：AIMTo在Descemet的家根据厚度评估视觉尖酸和endothelial房间密度剥去自动化endothelialkeratoplasty(DSAEK)在外科以后的年。

简介：AIMTo评估剥去与在没有胶囊的support.METHODSDSAEK的严重地损坏的眼睛的透镜(IOL)培植与工匠虹爪IOL培植相结合的工匠缺少晶状体intraocular相结合的自动化endothelialkeratoplasty(DSAEK)的descemet的复杂并发症和结果在29只眼睛上被执行。所有眼睛由于从优先的intraocular手术和眼睛的损伤的复杂并发症具有反常结构。眼睛的复杂并发症观察了包括的接枝脱臼，高intraocular压力(IOP)，IOL脱臼，有斑点的浮肿和hyphema。最好改正的视觉尖酸(BCVA)，IOP和吝啬的中央endothelial房间密度(ECD)是有的recorded.RESULTSThirteen眼睛眼睛的损伤的历史，10只眼睛有前面的房间IOL，16只眼睛有优先的vitrectomy。虹在22种情况中是反常的。接枝脱臼发生在5(17.2%)29只眼睛。IOL脱臼发生在2只眼睛(6.9%)。高IOP在9只眼睛被发现并且与处理被控制。外科手术前的吝啬的BCVA是20/286。6mo手术后的吝啬的BCVA是20/42。平均中心ECD是在6mo的1965.3房间/公里2，并且施主房间损失的率是与工匠缺少晶状体IOL培植相结合的34.7%.CONCLUSIONDSAEK是为没有胶囊的支持，在aphakic眼睛解决endothelial和透镜混乱的一种其他的选择。然而，它应该与严重的虹缺点为眼睛小心地被执行。

简介：这研究试图评估结果并且描述了cryopreservedlimbal的恢复过程为角膜的外设和limbal疾病的薄片状的keratoplasty(CLLK)。有一个平均数的12个病人的13只眼睛41变老

简介：Adultstem/progenitorcellsplayimportantrolesintissuehomeostasisandhaveimportantimplicationsforregenerativemedicine.Itwasoncethoughtthatformationofnewbloodvesselsinadultonlyoccursthroughangiogenesis,aprocesswherebynewvesselsareformedfromexistingmatureendothelialcells;whilevasculogenesis,wherenewvesselsarederivedfromdifferentiationofendothelialprogenitorcells(EPCs),wasthoughttooccurexclusivelyinembryos.ThediscoveryofadultEPCsafewyearsagohaschangedthisoldparadigm;andsubsequentstudiesshowedthatEPCsmaybeapromisingtoolforthetreatmentofvasculardisorders.However,therehavebeenconflictingreportsonsubtypes,surfacemarkers,andfunctionsofEPCs;andthustheexactoriginandidentityofEPCsremaintobedefined.AcommonapproachtoobtainEPCsistoisolateandculturemononuclearcellsfromperipheralbloodandtoselectadherentcellsfor

简介：AIM:Toevaluatetheindications,complications,andvisualandgraftsurvivaloutcomesineyesthathadundergonesimultaneouscataractextractionandpenetratingkeratoplasty(PKP).·METHODS:Wedescribedaretrospectivestudyof101patientswhohadundergonesimultaneouscataractextractionandPKPatKingKhaledEyeSpecialistHospitalbetweenJanuary1,2001,andDecember31,2002.Allpatientswerefolloweduppostoperativelywithmaximumfollow-up68months.·RESULTS:Themeanageofpatientswas61years.Themeanoverallfollow-upwas27months.Themostcommonindicationsforsurgerywerecornealscarring(45.5%),previousfailedgraft(15.8%),cornealulcer(12.9%),Fuchsendothelialdystrophy(8.9%),stromaldystrophy(2.9%),andotherconditions(14.5%).Overall,69grafts(68.3%)remainedclearatfinalfollow-up.Previousglaucomaorpostoperativeglaucomahadnostatisticallysignificanteffectsongraftoutcome(P>0.05).Thegraftrejectionrate(17.8%)wasasignificantriskfactorforfailure(P=0.00).Age,gender,indicationsforsurgery,cornealgraftdiameter,andintraoperativevitreouslosshadnostatisticallysignificanteffectsonthePKPoutcome(P>0.05).Postoperativevisualacuitywassignificantlyassociatedwithpreoperativevisualacuity(P<0.01).·CONCLUSION:Thepresentstudyprovidesevidencethatperformingacombinedprocedureresultsinmorerapidvisualrehabilitationandgoodgraftclarity.

简介：AbstractFluid resuscitation is an essential intervention in critically ill patients, and its ultimate goal is to restore tissue perfusion. Critical illnesses are often accompanied by glycocalyx degradation caused by inflammatory reactions, hypoperfusion, shock, and so forth, leading to disturbed microcirculatory perfusion and organ dysfunction. Therefore, maintaining or even restoring the glycocalyx integrity may be of high priority in the therapeutic strategy. Like drugs, however, different resuscitation fluids may have beneficial or harmful effects on the integrity of the glycocalyx. The purpose of this article is to review the effects of different resuscitation fluids on the glycocalyx. Many animal studies have shown that normal saline might be associated with glycocalyx degradation, but clinical studies have not confirmed this finding. Hydroxyethyl starch (HES), rather than other synthetic colloids, may restore the glycocalyx. However, the use of HES also leads to serious adverse events such as acute kidney injury and bleeding tendencies. Some studies have suggested that albumin may restore the glycocalyx, whereas others have suggested that balanced crystalloids might aggravate glycocalyx degradation. Notably, most studies did not correct the effects of the infusion rate or fluid volume; therefore, the results of using balanced crystalloids remain unclear. Moreover, mainly animal studies have suggested that plasma may protect and restore glycocalyx integrity, and this still requires confirmation by high-quality clinical studies.

简介：在分数之间的关系在风险分数系统(RSS)获得了的AIMTo分析与渗透keratoplasty(PKP)由希克斯等求婚了在1y的接枝失败手术后地并且在在有PKP接枝失败的风险的RSS的每个因素之中使用univariate和multivariateanalysis.METHODSThe有的回顾的队学习152从152个病人的PKP。十八个盒子由于主要失败(10个盒子)从我们的学习被排除，不完全的医药笔记(5个盒子)和后续不到1y(3个盒子)。我们从134个病人包括了134PKP在外科手术前的风险分数成层。枪兵系数在1y为在获得的分数和失败的风险之间的关系被计算。Univariate和multivariate分析为在1y.RESULTSSpearman系数在接枝失败上在RSS包括的每个单个风险因素的影响被计算在在RSS和接枝失败的分数之间的显示出的统计上重要的关联(P<0.05)。Multivariate逻辑回归分析没显示出统计上重要的关系(P>0.05)在有接枝失败的诊断和透镜地位之间。在因素学习了的另外的风险和接枝失败之间的关系是重要的(P<0.05)，尽管以前的接枝的结果和接枝失败是不可靠的。这样，任何一个都没我们的病人有以前的输血它没有impact.CONCLUSIONAftermultivariate分析技术的申请，一些风险因素不在1y在接枝失败上显示出期望的影响。

简介：<正>DearSir,IamJinAChoi,fromtheDepartmentofOphthalmologyofSt.VincentHospitalofSuwon,Kyungki-do,SouthKorea.IwritetopresentacasereportofayoungfemaletreatedwiththediagnosisofPosner-Schlossmansyndromeforseveralyears,inwhichshewasultimatelyprovedtohaveiridocornealendothelial(ICE)syndrome.Posner-Schlossmansyndrome(PSsyndrome)ischaracterizedbyrecurrentattacksofmildanterioruveitiswithmarkedelevationofintraocularpressure(IOP)[1].Theexactetiologyofthissyndromeisnotfullyestablishedyet.However,thepossibleroleofherpessimplexvirus(HSV)issuggestedbyastudythatrevealedDNAevidenceofthevirusinallaqueousspecimensofpatientsduringacuteattacksinPSsyndrome[1,2].ICEisaspectrumofdiseasethat

简介：Intracerebralhemorrhage(ICH)isthemostseverecerebrovasculardisease,whichrepresentsaleadingcauseofdeathanddisabilityindevelopedcountries.However,therapeuticoptionsarelimited,soismandatorytoinvestigaterepairingprocessesafterstrokeinordertodevelopnewtherapeuticstrategiesabletopromotebrainrepairprocesses.TherapeuticangiogenesisandvasculogenesisholdpromisetoimproveoutcomeofICHpatients.Inthisregard,circulatingendothelialprogenitorcells(EPCs)haverecentlybeensuggestedtobeamarkerofvascularriskandendothelialfunction.Moreover,EPClevelshavebeenassociatedwithgoodneurologicalandfunctionaloutcomeaswellasreducedresidualhematomavolumeinICHpatients.Finally,experimentalandclinicalstudiesindicatethatEPCmightmediateendothelialcellregenerationandneovascularization.Therefore,EPC-basedtherapycouldbeanexcellenttherapeuticoptioninICH.Inthismini-review,wediscussthepresentstatusofknowledgeaboutthepossibletherapeuticroleofEPCsinICH,molecularmechanisms,andthefutureperspectivesandstrategiesfortheiruseinclinicalpractice.

简介：ＴＨＥＰＲＯＰＥＲＴＩＥＳＯＦＬＥＵＫＯＣＹＴＥＡＮＤＥＲＹＴＨＲＯＣＹＴＥＡＤＨＥＲＥＤＴＯＥＮＤＯＴＨＥＬＩＡＬＣＥＬＬＩＮＦＬＯＷＦＩＥＬＤＴＨＥＰＲＯＰＥＲＴＩＥＳＯＦＬＥＵＫＯＣＹＴＥＡＮＤＥＲＹＴＨＲＯＣＹＴＥＡＤＨＥＲＥＤＴＯＥＮＤＯＴ...

简介：AIMTo在角膜的endothelial房间(CEC)并且到的增长上探索调节媒介的效果比较不同调节媒介(厘米)的效率.METHODSRatCEC，角膜的stromal房间(CSC)，骨头导出髓的endothelial祖先房间(BEPC)，并且骨头导出髓的间充质的干细胞(BMSC)被孤立并且在vitro有教养。厘米从CSC，BEPC，和BMSC被收集。CEC在不同文化媒介被栽培。房间形态学被记录，并且基因和蛋白质表示是为5d在厘米种的analyzed.RESULTSAfter，在每个试验性的组的CEC仍然保持多角形，在像鹅卵石的单层安排。Immunocytofluorescence揭示了Na+/K+-ATP,aquaporin的积极表示1(AQP1)，并且zonulaoccludens1(ZO-1)。把分析基于量的聚合酶链反应(qPCR)，在CSC厘米的Na+/K+-ATP表示是尤其是由1.3褶层的upregulated(±；0.036)(P<；0.05，n=3)。ZO-1的表示层次，神经原特定的enolase(NSE)，Vimentin，配对的homebox6(PAX6)，并且procollagen类型VIII(COL8A1)是尤其是在每个试验性的组的upregulated。每厘米在CEC增长上有积极效果，并且CSC厘米在proliferation.CONCLUSIONCSC厘米，BEPC厘米，和BMSC厘米上有最强壮的效果不仅刺激了CEC的增长，而且坚持说特征区分了为endothelial功能必要的显型。CSC厘米在CEC增长上有最著名的效果。

简介：AIM:Totransfectthecatcornealendothelialcells(CECs)withrecombinanthumanβ-nervegrowthfactorgeneadeno-associatedvirus(AAV-β-NGF)andtoobservetheeffectoftheexpressedβ-NGFproteinontheproliferationactivityofcatCECs.METHODS:TheendotheliumofcatcorneawastornunderthemicroscopeandrapidlycultivatedinDulbecco’smodifiedEagle’smedium(DMEM)toformsinglelayerCECsandthepassage2endothelialcellswereusedinthisexperiment.TherecombinanthumanAAV-β-NGFwasconstructed.TherecombinanthumanAAV-β-NGFwastransferredintocatCECsdirectly.Threegroupswereasfollowing:normalCECcontrolgroup,CEC-AAVcontrolgroupandrecombinantCECAAV-β-NGFgroup.Forty-eighthoursaftertransfection,thetotalRNAwasextractedfromtheCECbyTrizol.Theexpressionoftheβ-NGFtargetgenedetectedbyfluorescencequantitativepolymerasechainreaction;proliferationactivityofthetransfectedCECdetectedat48hbyMTTassay;thepercentageofG1cellsamongCECsaftertransfectwasdetectedbyflowcytometrymethod(FCM);cellmorphologywasobservedunderinvertedphasecontrastmicroscope.RESULTS:Thetornendotheliumculturetechniquerapidlycultivatedsinglelayercatcornealendothelialcells.Theself-designedprimersforthetargetgeneandreferencegenewereefficientandspecialconfirmedthroughelectrophoresisanalysisandDNAsequencing.Forty-eighthoursaftertransfect,thehumanβ-NGFgenemRNAdetectedbyfluorescencequantitativepolymerasechainreactionshowedthattherewasnosignificantdifferencebetweennormalCECcontrolgroupandCECAAVcontrolgroup(P>0.05);therewassignificantdifferencebetweentwocontrolgroupsandrecombinantCEC-AAV-β-NGFgroup(P<0.05).MTTassayshowedthattransfectofrecombinantAAV-β-NGFpromotedtheproliferationactivityofcatCEC,whiletherewasnosignificantdifferencebetweennormalCECcontrolgroupandCEC-AAVcontrolgroup(P>0.05).FCMresultshowedthatthepercentageofG1cellsinCECAAV-NGFgroupwas76.8%whi

简介：AbstractObjective:The role of Vitamin D-binding protein (DBP) in preeclampsia (PE) pathogenesis is unknown. In this study, we compared the expression of DBP in the placentas of PE patients with the placentas of normotensive pregnant women with placenta previa controls, and aimed to explore the effect of DBP on endothelial cells (ECs) and the underlying mechanism.Methods:DBP expression in placental tissues collected from PE patients and controls was evaluated by immunohistochemistry. The downregulation and upregulation of DBP expression in HTR-8/SVneo cells were examined using DBP-targeting small interfering RNA (siRNA) and DBP-expression vector, respectively. The conditioned media of these DBP-overexpressing and DBP-siRNA HTR-8/SVneo cells were collected and added to human umbilical vein EC (HUVEC) cultures. Angiogenic effects on HUVECs were assessed by tube formation assays, and the proliferation and migration of HUVECs were examined using the Real-Time Cell Analyzer. The expression of vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR)-2, as well as the phosphorylation of different residues of VEGFR-2 in HUVECs, were determined by western blotting.Results:DBP expression was significantly increased in the placental tissues collected from PE patients. The conditioned medium of DBP-overexpressing HTR-8/SVneo cells potently inhibited tube formation by HUVECs, in addition to their proliferation and migration. Furthermore, treatment of HUVECs with the conditioned medium of DBP-overexpressing HTR-8/SVneo cells decreased the phosphorylation of VEGFR-2 at tyrosine 996, whereas the treatment of these cells with the conditioned medium of DBP-siRNA HTR-8/SVneo cells increased the phosphorylation of VEGFR-2 at tyrosine 951, 996, and 1,175.Conclusions:The expression of DBP is increased in the placentas of PE patients. DBP plays potential roles in endothelial dysfunction, which contributes to PE development, by inhibiting tyrosine phosphorylation of VEGFR-2 in ECs.

简介：Staphylococcusepidermidisisoneoftheleadingpathogensofnosocomialinfections.Twenty-eightstrainsofbiofilm-negativeStaphylococcusepidermidis,ofwhichthirteenstrainsconsideredastheclinicalstrainswereisolatedfromcatheters,bloodandurineofpatientsinHuashanHospital(Shang-hai,China)andfifteenstrainsconsideredascommensalstrainswereisolatedfromtheskinofhealthystudentswhohadnocontactwithhospitalsinrecenthalfyear,wereinvestigatedfortheadherencetohumanumbilicalveinendothelialcelllineECV304andhumancervicaladenocareinomacelllineHela,respectively.Ourresultsshowedthattheclinicalstrainsweremuchmoreadhesivetobothcellsthancommensalones.Theendothelialadherencemaybeavirulencefactorassociatedwiththisbacterialpathogenesis.

简介：

简介：ObjectivesTotestthehypothesesthatbonemorphogeneticprotein(BMP)signalingpathwaycomponentsareexpressedinarterialendothelialcells(ECs)andthatBMPsignalinginfluencesendothelialcell(EC)proliferation.MethodsWeusedcellcultureandRT-PCRtodeterminedmRNAexpressionofBMPreceptors(BMPR)-IA,-IB,andII,Smads1,4,5,6,and7,andinculturedhumancoronaryarteryECsatbaselineandafterstimulationwithBMP2(300ng/mLfor6hr),non-radioactivecellproliferationtoexaminecellproliferation.ResultsProteasomeinhibitionhasbeenpreviouslyshowntoenhanceBMPsignalingbypreventingdegradationofBMPpathwaycomponents.Therefore,identicalexperimentswerealsoperformedinthepresenceoftheproteasomeinhibitorepoxomicin.ECsexpressedmRNAforBMPRsIAandII,Smads1,4,5,6,7,andstimulationwitheitherBMP2orepoxomicinresultedinasignificantincreaseinECproliferationmeasuredafter48hoursinadose-dependentfashion.Proliferationwasaccompaniedbyamarkedincreaseinproliferatingcellnuclearantigen(PCNA)expression.Toxicitywasobservedathighdosesofepoxomicin.ConclusionsAllmajorBMPsignalingmoleculesareexpressedbyvascularECsandexpressionoftheseareaffectedbybothBMP2andepoxomicin.BMP2mayregulateECproliferation,suggestingapossibleroleinvascularhomeostasisandvascularpathologiesinvolvingECdenudationorproliferation.

简介：Objective:Toinvestigatethebindingcharacteristicsofendothelialcell(EC)withLPSfreefromtheparticipationofserumfactors.Methods:LaserconfocalmicroscopewasemployedintheobservationofthebindingofECwithFITC-LPS.TheKDandthebindingsitesofeachECwerecalculatedbyradioligandbindingassayofreceptors(RBA)using[3H]-LPS.Results:ThebindingofECwithLPSwassaturable,timeandconcentrationdependentanditcouldbecompetedwithoverdosedLPSofthesametype.Thefluorescencemainlydistributedincytoplasm,especiallynearthenucleus,whichcouldalsobestained.Conclusions:TheremightbesomespecificLPSbindingsitesexistingonEcsandLPScouldfunctionintracellularily.

简介：Insufficientgrowthandrarefactionofcapillaries,followedbyendothelialdysfunctionmayrepresentoneofthemostcriticalmechanismsinvolvedinheartdamage.Inthisstudyweexaminedhistochemicalandultrastructuralchangesinmyocardialcapillaryendotheliumintwomodelsofheartfailurestreptozotocin-induceddiabetesmellitus(STZ)andNOdeficienthypertensioninmaleWistarrats.Diabeteswasinducedbyasinglei.v.doseofSTZ(45mg/kg)andchronic9-weekstagewasanalysed.ToinduceNO-deficienthypertension,animalsweretreatedwithinhibitorofNOsynthaseLnitroargininemethylester(L-NAME)(40mg/kg)for4weeks.Leftventriculartissuewasprocessedforenzymecatalytichistochemistryofcapillaryalkalinephosphatase(AlPh),dipeptidylpeptidaseⅣ(DPPⅣ),andendothelialNOsynthase/NADPH-diaphorase(NOS)andforultrastructuralanalysis.Indiabeticandhypertensiverats,lower/absentAlPhandDPPⅣactivitieswerefoundinfocalmicro-areas.NOSactivitywassignificantlyreducedandpersistedonlylocally.QuantitativeevaluationdemonstratedreductionofreactionproductintensityofAlPh,DPPandNOSby49.50%,74.36%,20.05%indiabeticand62.93%,82.71%,37.65%inhypertensiverats.Subcellularalterationsofendothelialcellswerefoundinheartofbothgroupssuggestinginjuryofcapillaryfunctionaswellascompensatoryprocesses.Endothelialinjurywasmoresignificantindiabeticanimals,incontrasttheadaptationwasmoreevidentinhypertensiveones.Concluding:bothSTZ-induceddiabetes-andNO-deficienthypertension-relatedcardiomyopathywereaccompaniedbysimilarfeaturesofstructuralremodellingofcardiaccapillarynetworkmanifestedasangiogenesisandangiopathy.Thelatterwashowever,predominantandmayacceleratedisappearanceofcapillaryendotheliumcontributingtomyocardialdysfunction.

简介：AIM:Toexploretheeffectsandmechanismofvascularendothelialcadherin(VE-cadherin)onexperimentalcornealneovascularization(CRNV).·METHODS:MousecorneaswereburnedwithsodiumhydroxidetobuildaCRNVmodel.Theburnedcorneaswerelocallyadministratedwithanti-mouseVE-cadherinneutralizingantibody.AnnexinVandclusterofdifferentiation31(CD31)doublestainingwasusedtomeasurevascularendothelialcellapoptosiswiththeuseofflowcytometry(FCM).TheproteinexpressionofNADPHoxidase2(Nox2),caspase-3,andproteinkinaseC(PKC)intheburnedcorneaswereexaminedbyWesternblot.Humanretinalendothelialcell(HREC)proliferationwasdetectedusingaCellCountingKit8(CCK-8)assayinvitro.·RESULTS:TheamountofCRNVpeakedtwoweeksafterthealkaliburn.FCMconfirmedthatVE-cadherinneutralizingantibodytreatmentincreasedCD31positivecellapoptosis.WesternblotrevealedthattheintracornealproteinexpressionofNox2andcaspase-3wereup-regulated,whilePKCwasdown-regulatedintheVE-cadherinneutralizingantibodyadministratedgroup.CCK-8assayshowedthatVE-cadherinneutralizingantibodymarkedlyinhibitedHRECproliferation.·CONCLUSION:VE-cadherinexhibitedananti-apoptosiseffectthroughenhancedPKCsignalingandanenhancedcellproliferationpathway.