简介：ObjectivesToestablishamethodforhighyieldmesenchymalstemcellscollection,aswellasaculturemethodforidentifyingmesenchymalstemcellsfromtheswineadipose-derivedmesenchymalstemcell(ADMSC).MethodsSwineADMSCswereisolatedfromfattissuewithcollagenase,followedbyinductionofdifferentiationtoosteogenic,adipogenicandchondrogrniccells.ThesurvivalcurveoftheADMSCatthe37oCand38oCweremeasuredusingWST-1CellProliferationAssayReagent.ResultADMSCsisolatedwithcollagenasefromswineneckfattissuegeneratedastableuniformappearanceafterthesecondgeneration.Thepassageperiodwasfivedays.ADMSCcoulddifferentiateintoosteogenic,adipogenicorchondrogrniccellsunderdifferentcultureconditions.Thehighestgrowthratewasachievedat38oCinthisstudy.ConclusionSwineADMSCshavethepotentialtodifferentiateintoosteogenic,adipogenicorchondrogrniccells,andtheymaybeappropriatefortransplantationforbothresearchandclinicalpurpose.

简介：ObjectiveAdipose-derivedstemcells(ADSCs)aresuggestedtopossessahighlyplasticabilitytodifferentiateintoseveralspecificcelltypesinadditiontoadipocytelineages,includinggermlayertissue-specificcelllineagessuchaschondrocyte,myocyte,neuronal,andosteoblastlineages.TheaimofthisstudyistoestablishaninvitroculturetechniqueforADSCsinanadultguineapigmodelthatfacilitatetheirdifferentiationintohaircell-likecells.MaterialsandMethodsCellsfrominguinalfatpadsinadultguineapigswereculturedwithβ-mercaptoethanol,RA,Forskorin,Heregulin,bFGF,BDNFandEGF.Cellulardifferentiationwasexaminedusingimmunocytochemistrytechniques.ResultsTheADSCsdemonstratedhaircellimmunophenotypeswithexpressionofepitopesofthehaircellmarkerproteinmyosinⅦa.ConclusionADSCsfromadultguineapigadiposetissuecandifferentiateintohaircell-likecellswhenculturedinvitro.ADSCsmayserveasseedcellsfortissueengineering.