简介:Inordertocreateanewmimicofglutathioneperoxidase(GPx),bioimprintingwasusedtogenerategluta-thione(GSH)bindingsiteandchemicalmodificationwasusedtoincorporatecatalyticgroupselenocystine(Sec).Humanserumalbumin(HSA)andS-substituteddinitrophenylglutathione(GSH-S-DNP)werechosenastheimprintedmatrixandimprintingtemplate,respectively,togenerateaGSH-imprintedprotein(GSH-HSA)bybioimprinting.SecwasincorporatedintotheGSH-HSAbychemicalmodificationtogiveanewGPxmimic(Se-GSH-HSA).Se-GSH-HSAdisplayedconsiderablyhigherGPxactivitythannon-printedHSA(Se-HSA).TheenzymicpropertiesandkineticsofSe-GSH-HSAwerestudied.Moreover,Se-GSH-HSAwasconfirmedtohavestrongerantioxidantabilitytoprotectmitochondriaagainstoxidativedamagewithferroussulfate/ascorbate-inducedmitochondriadamagemodel,indicatingthatSe-GSH-HSAhaspotentialapplicationinmedicine.
简介:AIM:Tounderstandthecorrelationofserumcholinesterase(CHE)activitywithgastriccancerandtoassesstheirclinicalsignificance.METHODS:ThevelocitymethodwasadoptedtodetecttheactivityofserumCHEinpatientswithgastriccancerandinpatientswithnon-malignanttumorascontrols.RESULTS:TheserumCHEactivityinthetreatmentgroupwassignificantlylowerthanthatinthecontrolgroupwithaverysignificantdifferencebetweenthetwogroups(83.3:113.1,P=0.0003).Agewassignificantlyassociatedwiththeincidenceofgastriccaner.CONCLUSION:SerumCHEactivityhasacloserelationwiththeincidenceofgastriccancer.
简介:Objective:Toevaluatetheclinicalutilityoftestingserumanti-treponemapallidumIgMantibodyinthediagnosisofsyphilispatients.Methods:Seventy-twocasesofsyphilisweretestedforspecificIgMantibodywithELISA,andtheresultswerecomparedwithRPRandTPPA.Results:ThesensitivityofIgMantibodywas73.3%(11/15)inprimarysyphilis,88.9%(16/18)insec-ondarysyphilis,andtherewasnosignificantdiffer-encebetweenthesevalues(x^2=1.6363,P>0.10).ThesensitivityofIgMantibodyindiagnosinglatentsyphi-liswasonly26.1%(6/23),muchlowerthanthedetec-tionrateinsymptomaticearlvsvDhilis(x^2=17.6189.P<0.005).RPRandTPPAwereboth100%sensitiveinlatentandearlysymptomaticsyphilis.Twowereposi,fiveforIgMinthe16caseswhohadreceivedregulartreatments2to24monthsbeforeenrolled.Conclusions:SpecificIgMantibodydetectiondoeesnotappearsuperiortoRPRandTPPAindiagnosingprimarysyphilis.ThediagnosisoflatentsyphilisshouldmainlyrelyonRPRandTPPA,sincetherearelowtitersofIgMantibodyatthatstage.IgMantibodytestingaloneshouldnotberecommendedformonitor-ingsyphilisdevelopmentortreatmentefficacy.Fur-therstudiesshouldbeconcerned.
简介:AbstractBackground:Whether there is an association between serum uric acid (SUA) level and risk of mortality in the general population remains unclear. Based on the China National Survey of Chronic Kidney Disease linked to mortality data, a population-based cohort study was performed to investigate the association between SUA level and all-cause mortality, cardiovascular disease (CVD) mortality, and cancer mortality in China.Methods:The survival status of participants in the cross-sectional survey was identified from January 1, 2006 to December 31, 2017. Only 33,268 individuals with complete SUA data among the 47,204 participants were included in the analysis. We determined the rates of all-cause mortality, CVD mortality, and cancer mortality. We used Cox proportional hazards regression models to evaluate the effect of the SUA level on mortality.Results:During a total of 297,538.4 person-years of follow-up, 1282 deaths occurred. In the Cox proportional hazards regression model, the rate of all-cause mortality, CVD mortality, and cancer mortality had a U-shaped association with SUA levels only in men, whereas no significant associations were detected in women. For all-cause mortality in men, the multivariable-adjusted hazard ratios (HRs) in the first, second, and fourth quartiles compared with the third quartile were 1.31 (95% confidence interval [CI] 1.04-1.67), 1.17 (95% CI 0.92-1.47), and 1.55 (95% CI 1.24-1.93), respectively. For CVD mortality, the corresponding HRs were 1.47 (95% CI 1.00-2.18), 1.17 (95% CI 0.79-1.75), and 1.67 (95% CI 1.16-2.43), respectively. For the cancer mortality rate, only a marginally significant association was detected in the fourth quartile compared with the third quartile with an HR of 1.43 (95% CI 0.99-2.08).Conclusions:The association between SUA and mortality differed by sex. We demonstrated a U-shaped association with SUA levels for all-cause and CVD mortalities among men in China.
简介:Thisstudysoughttoexamineneuroglobin(NGB)intheserumofacutecerebralinfarctionpatientswithdouble-antibodysandwichenzyme-linkedimmunosorbentassaytoidentifyallriskfactors,calculateinfarctsize,assessneurologicalimpairment,andanalyzetherelationbetweenNGBandeachofthesefactors.Thedouble-antibodysandwichassayindicatedthatlevelsofNGBinserumwereunalteredwithin6hoursfollowingacutecerebralinfarctioncomparedwithnormallevels.NGBlevelsthenunderwentadistinctchange,peakingat24hoursthenreturningtonormallevelsin72hours.TheresultssuggestthatthelevelofNGBmightberelatedtoinfarctsizeandlow-densitylipoproteinat24hoursafteracutecerebralinfarction.Therewerenosignificantdifferencesinneurologicalimpairmentscoresandinfarctsizeatdifferentperiodsfollowinginfarction.ThefindingsindicatedthatthelevelofNGBinserumofacutecerebralinfarctionpatientswascorrelatedwithinfarcttime.
简介:AbstractBackground:Vancomycin treatment failure against vancomycin-susceptible gram-positive cocci is not rare in the intensive care unit (ICU). One of the reasons for this is the substandard drug trough concentration. We aimed to examine the hypothesis that the target serum concentration could be reached earlier with a loading dose of vancomycin.Methods:This retrospective cohort study was conducted at our ICU between June 2018 and June 2020 and involved patients who were suspected of having, or confirmed to have, gram-positive cocci infection and treated with vancomycin. One group of the patients was administered a loading dose of vancomycin (loading group) and compared with the group that did not receive a loading dose (control group). The baseline characteristics, vancomycin serum concentrations, and clinical outcomes were collected and analyzed.Results:Fifty-five patients were finally included, of which 29 received a loading dose of vancomycin. The serum concentration of vancomycin before the second dose was significantly higher for the loading group than for the control group (10.3 ± 6.1 mg/L vs. 5.7 ± 4.4 mg/L, P = 0.002). The results for both groups were similar before the fifth dose (12.4 ± 7.3 mg/L vs. 10.3 ± 6.3 mg/L in the loading and the control groups, respectively; P = 0.251). The 28-day mortality was lower for the loading group than for the control group (6.7% vs. 34.6% in the loading and control groups, respectively; P = 0.026). No significant differences were observed in serum creatinine (Cr) concentrations of the two groups.Conclusion:With the loading dose of vancomycin, the target serum concentration of vancomycin may be reached earlier without increasing the risk of acute kidney injury.Trial registration:https://www.chictr.org.cn; ChiCTR2000035369
简介:ToexploretherelationbetweenserumbilinabinandeoronaryheartdiseaseMethodsComparethelevelofserumbilinabinamongpatientswitheoronaryheartdisease,patientswithotherdiseaseandnormalpersons.ResultsThelevelofserumbilinabinofpatientswithcoronaryheartdiseaseishigherthanthatofnormalpersons.ConclusionThereductionofdensityofserumbihrubinisoneoftheindependentriskfactorsofcoronaryheartdisease.