简介:OptimalformationreconfigurationcontrolofmultipleUninhabitedCombatAirVehicles(UCAVs)isacomplicatedglobaloptimumproblem.ParticleSwarmOptimization(PSO)isapopulationbasedstochasticoptimizationtechniqueinspiredbysocialbehaviourofbirdflockingorfishschooling.PSOcanachievebetterresultsinafaster,cheaperwaycomparedwithotherbio-inspiredcomputationalmethods,andtherearefewparameterstoadjustinPSO.Inthispaper,weproposeanimprovedPSOmodelforsolvingtheoptimalformationreconfigurationcontrolproblemformultipleUCAVs.Firstly,theControlParameteri-zationandTimeDiscretization(CPTD)methodisdesignedindetail.Then,themutationstrategyandaspecialmutation-escapeoperatorareadoptedintheimprovedPSOmodeltomakeparticlesexplorethesearchspacemoreefficiently.Theproposedstrategycanproducealargespeedvaluedynamicallyaccordingtothevariationofthespeed,whichmakesthealgorithmexplorethelocalandglobalminimathoroughlyatthesametime.SeriesexperimentalresultsdemonstratethefeasibilityandeffectivenessoftheproposedmethodinsolvingtheoptimalformationreconfigurationcontrolproblemformultipleUCAVs.
简介:Afterpre-cultureandtreatmentofosmosis,cotyledonsofimmaturepeanut(ArachishypogaeaL.)zygoticembryosweretransformedviaparticlebombardmentwithaplasmidcontainingachimerichphgeneconferringresistancetohygromycinandachimericintron-gusgene.Selectionforhygromycinresistantcallusesandsomaticembryoswasinitiatedat10thdpost-bombardmentonmediumcontaining10-25mg/Lhygromycin.Undercontinuousselection,hygromycinresistantplantletswereregeneratedfromsomaticembryosandwererecoveredfromnearly1.6%ofthebombardedcotyledons.ThepresenceandintegrationofforeignDNAinregeneratedhygromycinresistantplantswasconfirmedbyPCR(polymerasechainreaction)fortheintron-gusgeneandbySouthernhybridizationofthehphgene.GUSenzymeactivitywasdetectedinleafletsfromtransgenicplantsbutnotfromcontrol,non-transformedplants.Theproductionoftransgenicplantsaremainlybasedonanewlyimprovedsomaticembryogenesisregenerationsystemdevelopedbyus.
简介:Heterogeneousnuclearribonucleoproteins(hnRNPs)arespliceosomalmacromolecularassemblagesandthusactivelyparticipateinpre-mRNAmetabolism.Theyarecomposedofevolutionarilyconservedandtandemlyrepeatedmotifs,wherebothRNA-bindingandprotein-proteinrecognitionoccurtoachievecellularactivities.Byyetunknownmechanisms,theseribonucleoprotein(RNP)particlesaretargetedbyautoantibodiesandhenceplaysignificantroleinavarietyofhumansystemicautoimmunediseases.Thisfeaturemakesthemimportantprognosticmarkersintermsofmolecularepidemiologyandpathogenesisofautoimmunity.SinceRNPdomainisoneofthemostconservedandwidespreadscaffolds,evolutionalysesoftheseRNA-bindingdomainscanprovidefurthercluesondisease-specificepitopeformation.ThestudypresentedhereinrepresentsasequencecomparisonofRNA-recognitionregionsofrecentlyclonedandcharacterizedhumanhnRNPA3withthoseofotherrelevanthnRNPA/B-typeproteins.Theirimplicationsinhumanautoimmunityareparticularlyemphasized.