简介：AbstractIntroduction:Neuroblastoma (NB) is the most common extracranial solid tumor among children. The 5-year event-free survival rate for high-risk (HR) NB is still poor, especially for patients with advanced NB with MYCN gene amplification. Chimeric antigen receptor T (CAR-T) cell therapy is a new treatment for HR-NB.Case presentation:A 55-month-old boy with stage IV HR-NB received 4th-generation CAR-T cells that target disialoganglioside GD2, as consolidation maintenance treatment after intensive chemotherapy, surgery, and autologous stem-cell transplantation. As of February 2019, his CAR-T follow-up time was 37.5 months, indicating prolonged survival. Cranial MRI and ultrasound showed no mass; 123I-metaiodobenzylguanidine (123I-MIBG) scan was negative.Conclusion:GD2-CAR-T cells may be an effective treatment option for NB patients with MYCN amplification.

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简介：Thepulseamplificationinthedispersion-decreasingfiber(DDF)isinvestigatedviasymboliccomputationtosolvethevariable-coefficienthigher-ordernonlinearSchr(o|¨)dingerequationwiththeeffectsofthird-orderdispersion,self-steepening,andstimulatedRamanscattering.Theanalyticone-solitonsolutionofthismodelisobtainedwithasetofparametricconditions.Basedonthissolution,thefundamentalsolitonisshowntobeamplifiedintheDDF.ThecomparisonoftheamplitudeofpulsesfordifferentdispersionprofilesoftheDDFisalsoperformedthroughthegraphicalanalysis.Theresultsofthispaperwouldbeofcertainvaluetothestudyofsignalamplificationandpulsecompression.

简介：Inthispaper,astructuralanalysisisperformedtogaininsightsonthesynergisticmechanicalamplificationeffectthatCampaniformsensillahavewhencombinedinanarrayconfiguration.Inordertosimplifytheanalysisperformedinthispreliminaryinvestigation,anarrayoffourholesinasingleorthotropiclaminaisconsidered.Firstly,aFiniteElementMethod(FEM)analysisisperformedtodiscretelyassesstheinfluencethatdifferentgeometricalparametershaveonthemechanicalamplificationpropertiesofthearray.Secondly,anartificialneuralnetworkisusedtoobtainanapproximatedmulti-dimensionalcontinuousfunction,whichmodelstherelationshipbetweenthegeometricalparametersandtheamplificationpropertiesofthearray.Thirdly,anoptimizationisperformedtoidentifythegeometricalparametersyieldingthemaximummechanicalamplification.Finally,resultsarevalidatedwithanadditionalFEMsimulationperformedbyvaryinggeometricalparametersintheneighborhoodoftheidentifiedoptimalparameters.ThemethodproposedinthispapercanbefullyautomatedandusedtosolveawiderangeofoptimizationproblemsaimedatidentifyingoptimalconfigurationsofstrainsensorsinspiredbyCampaniformsensilla.

简介：半活跃的水坝pers被使用在对还原剂基础隔离土木工程学结构的地震反应。在现在的学习，用可变扩大的一个新半活跃的抑制系统将被调查为适应基础隔离。它使用在系列连接与的一台新奇可变扩大设备(VAD)一被动更潮湿。VAD能够生产多重扩大因素，各相应于一个不同扩大状态。力量从更潮湿根据当前的扩大状态被放大到结构，它经由明确地定制到系统的一个半活跃的控制算法被选择“s唯一的抑制特征。为adaptivebase隔离表明VAD潮湿系统的有效性，数字模拟为三被进行，易于远、近地的地面的七故事的base-isolatedbuildings打手势。结果显示系统能与孤立底的大楼相比在反应完成重要减小没有更潮湿。建议系统也被发现表现很好与相比一典型半活跃更潮湿。

简介：Wetheoreticallyproposethestructureofall-solidphotonicbandgapfibertosupportLP01modeat980nmandfourmodesofLP01,LP11,LP02andLP21atCbands.Weresearchcharacteristicsofthephotonicbandgabandapplypho-tonicfiberasgainmediumtofiberlasers,finallyrealizingseparateamplificationofLP11andLP21high-ordermodeswithsingle-modestablepumplightofLP01modeat980nm.Besides,werealizeLPllmodeandLP21modelaserout-putofdual-wavelength.

简介：CMV特定的免疫为人的cytomegalovirus(HCMV)的控制是必要的感染。干细胞移植在hemopoietic系统的疾病的一个范围的管理广泛地被使用。病人们在早posttransplant时期深刻地是immunosuppressed，并且cytomegalovirus(CMV)的复活仍然是病态和死亡的一个重要原因。CMV特定的CD8+T房间克隆的采纳转移被显示了减少病毒的复活的率；然而，为采纳转移的房间的前vivo生产是集中、昂贵的劳动。我们这里用CMV特定的epitope肽报导一个修改肽刺激方法为CMV特定的CTL的产生刺激PBMC。这个方法允许CMV特定的CTL的有效扩大并且从一件单个血样品为FACS分析提供很多房间。显著地，它完成允许容易要比较并且顺序评估了的不同个人的数据的CD8+T房间染色的tetramer的高频率。因此，这条途径为潜在的采纳免疫疗法扩展并且选择高特性的限制HLA的CMV-pp65-reactiveT房间线。

简介：Westudytheparametricamplificationofelectromagneticallyinducedtransparency-assistedRydbergsix-andeight-wavemixingsignalsthroughacascadednonlinearopticalprocessinahotrubidiumatomicensembleboththeoreticallyandexperimentally.Theshiftoftheresonantfrequency（inducedbytheRydberg-Rydberginter-action）ofparametricallyamplifiedsix-wavemixingsignalisobserved.Moreover,theinterplaysbetweenthedressingeffectsandRydberg-Rydberginteractionsinparametricallyamplifiedmultiwavemixingsignalsarein-vestigated.ThelinearamplificationofRydbergmultiwavemixingprocesseswithmultichannelnatureactsagainstthesuppressioncausedbyRydberg-Rydberginteractionanddressingeffect.

简介：摘要目的探讨MYCN对NK细胞的调控作用及其介导神经母细胞瘤（neuroblastoma，NB）细胞免疫逃逸机制。方法采用荧光原位杂交法（FISH）法和免疫组织化学法检测NB组织MYCN的表达情况，流式细胞术检测外周血NK细胞数量及比例、NK细胞的活化、NK细胞表面活化受体和NB细胞表面相应配体的表达状况；干扰/过表达NB细胞系MYCN，与分选的健康儿童NK细胞进行共培养，ELISA法检测共培养上清中细胞因子的分泌状况，4 h乳酸脱氢酶（LDH）释放法检测NK细胞对NB细胞的杀伤性。结果MYCN高表达NB患儿外周血NK细胞数量（F = 45. 53，P = 0. 0002）及比例（F = 43. 93，P = 0. 0003）显著减少，MYCN可显著抑制γ-干扰素、颗粒酶B和穿孔素的释放[SK-N-BE （2）：t = 9. 10，P = 0. 0008；t = 4. 76，P = 0. 0089；t = 4. 66，P = 0. 0096；SH-SY-5Y：t = 16. 79，P < 0. 0001；t = 35. 85，P < 0. 0001；t = 24. 66，P < 0. 0001]，抑制NK细胞的活化和对靶细胞的杀伤作用[SK-N-BE（2）：t = 7. 25，P = 0. 0019；SH-SY-5Y：t = 33. 07，P < 0. 0001]，MYCN高表达促进NK细胞NKG2A的表达[SK-N-BE（2）：t = 9. 25，P = 0. 0008；SH-SY-5Y：t = 14. 65，P = 0. 0001]，抑制NKG2D的表达[SK-N-BE（2）：t = 7. 21，P = 0. 0020；SH-SY-5Y：t = 8. 66，P = 0. 0010]，同时抑制NB细胞中NKG2D配体MICA[SK-N-BE（2）：t = 18. 35，P < 0. 0001；SH-SY-5Y：t = 56. 90，P < 0. 0001]和MICB[SK-N-BE（2）：t = 22. 40，P < 0. 0001]的表达。结论NB中高表达的MYCN可通过抑制NK细胞的数量及活性从而抑制NK细胞对NB细胞的杀伤介导NB细胞免疫逃逸。

简介：Cerenkovwakeamplificationcanbeusedasanacceleratingscheme,inwhichatriggerbunchofelectronspropagatinginsideacylindricalwaveguidefilledwithanactivemediumgeneratesaninitialwakefield.Duetothemultiplereflectionsinsidethewaveguide,thewakemaybeamplifiedsignificantlymorestronglythanwhenpropagatinginaboundlessmedium.Sufficientlyfarawayfromthetriggerbunchthewake,whichtravelswiththesamephasevelocityasthebunch,reachessaturationanditcanaccelerateasecondbunchofelectronstrailingbehind.ForaCO2gasmixtureournumericalandanalyticalcalculationsindicatethatashortsaturationlengthandahighgradientcanbeachievedwithalargewaveguideradiusfilledwithahighdensityofexcitedatomsandatriggerbunchthattravelsatavelocityslightlyabovetheCerenkovvelocity.Toobtainastablelevelofsaturatedwakethatwillbesuitableforparticleacceleration,itiscrucialtosatisfythesingle-moderesonancecondition,whichrequireshighaccuracyinthewaveguideradiusandtheratiobetweentheelectronphasevelocityandtheCerenkovvelocity.Forsingle-modepropagationourmodelindicatesthatitisfeasibletoobtaingradientsashighasGVm-1inawaveguidelengthofcm.

简介：在这份报纸，扩大因素ks偷看有在在山东省上的班和地点上的不同exceedance概率的地面运动被分析从班地点的358地上凿穿和班的140地上凿穿收集的土壤层的地震反应数据估计地点。从结果，一个人能结束那1ks的scatter阴谋通常服从正常分布；有输入地面的力量的增加的2ks减少打手势，它比在班lI地点在班地点是更明显的；3随磨擦运动输入的基岩接口的深度的增加，为类地点，ks逐渐地增加直到到稳定的值深度什么时候到达直到约20米或更大。然而为班地点，ks对深度感觉迟钝；4为班地点的ks的一般水准是1.47，比在中国GB18306-2001的地震地面运动参数带状配列地图使用了那稍微大。另外，在在在山东省上的山峰地面加速的不同层次的班和地点上的kspreliminarily在纸被讨论。

简介：Anumericaldesignonthetriangularphotoniccrystalfiber(PCF)basedbackwardmulti-pumpRamanamplifierispresented.ItisdemonstratedthathighflatRamangaincanbereachedbasedonPCF.InfluencesofdifferentgeometricparametersandgermaniumdopingconcentrationsontheRamannetgain,amplifiedspontaneousemission(ASE)noiseanddoubleRayleighbackscattering(DRBS)ofthesignalhavebeenanalyzed.ForoptimizingcrystalfiberRamanamplifier(FRA),thereistradeoffbetweenthegeometricparameterandgermaniumdopingconcentrationoftriangularPCF.TheresultsshowthatPCFisanappropriatecandidateforhighgainRamanamplifiers.

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简介：PastresearchershaveanticipatedtheoccurrenceofagreatearthquakeinthecentralHimalayasinthenearfuture.ThismaycauseseriousdamageintheKathmanduValley,whichsitsonanancientlakebedzone,withlacustrinesedimentsofmorethan500mdepth.Inthisstudy,thepredominantfrequencyofgroundmotionisevaluatedusingtheHorizontal-to-Vertical(H/V)spectralratiotechniqueandrecordingsofambientnoise.TheresultsoftheH/Vratioshowtwopeaksinabout20percentofthelocations,whicharedistributedmainlyinandaroundthecenterandnorthernpartoftheKathmanduValley.Thepredominantfrequenciesvaryfrom0.5Hzto8.9Hzinthestudyarea,whereasthesecondresonancefrequencyvariesfrom4Hzto6Hzinthecenterandnorthernpartofthevalley.Thisindicatesthatthecenterandnorthernpartofthevalleyhaveawiderangeofresonancefrequencyduetotwolevelsofimpedancecontrastonemaybefromthesurfacelayerandtheothermaybefromthelayerunderneath.ThesetwolevelsofresonanceindicatetheimportanceofconsideringtheeffectsofsurfaceandlowerlayersduringtheplanninganddesigningofinfrastructuresintheKathmanduValley.

简介：摘要MYCN基因在神经母细胞瘤（neuroblastoma，NB）细胞生长、凋亡、分化、浸润、转移及血管形成等方面发挥重要作用。临床中，MYCN基因扩增是NB危险度分级的重要指标之一。然而，MYCN基因检测面临检测方法操作复杂、检测成本高及不易获得瘤组织细胞等问题。研究表明，NB患儿血清循环MYCN基因扩增情况与瘤组织中一致，血清循环MYCN基因扩增情况作为NB预后及复发监测指标具有灵敏度高、特异性好、操作简便及无创等优势。因此，血清循环MYCN基因扩增检测有望替代瘤组织中MYCN基因扩增检测，用于NB预后及复发监测。本文通过介绍NB患儿血清循环MYCN基因扩增情况在NB诊断、预后及复发监测中的应用研究进展，并对其未来发展进行展望，以期推进其临床转化应用的速度。

简介：摘要目的通过生物信息学分析探究儿童神经母细胞瘤中与MYCN(N-myc)基因相关的共表达基因。方法下载TARGET数据库神经母细胞瘤表达数据共179例，WGCNA分析筛选MYCN扩增共表达基因模块；针对目标基因模块个基因行GO、KEGG富集分析筛选MYCN扩增相关基因可能参与生物过程及信号通路；以MYCN扩增状态将神经母细胞瘤表达数据分为扩增组65例、非扩增组114例，行差异表达基因分析；筛选的差异表达基因同MYCN扩增相关基因取交集后运用K-M法行生存分析(log-rank检验)，筛选预后相关基因，Logistics回归探究MYCN扩增状态同预后基因的关系。结果WGCNA分析提示MEgreenyellow模块同MYCN基因扩增状态相关性最强(R＝0.46，P<0.05)，为共表达基因，包含基因1 216个，富集分析提示MYCN扩增共表达基因多参与RNA相关通路(RNA的转运、剪切、修饰，RNA相关酶的活性等过程)；差异表达基因分析显示，MYCN扩增组相较于非扩增组，基因表达上调47个，表达下调123个；WGCNA分析同差异表达基因取交集筛选出22个关键基因，行生存分析显示同预后相关的基因(P<0.05)有12个，其中DDX1与其他关键基因相关性相对较弱，或为神经母细胞瘤独立预后因素。结论LINC00839、ATP结合盒亚家族C4(ABCC4)、亚甲基四氢叶酸脱氢酶(NADP+依赖)2(MTHFD2)、磷酸甘油酸脱氢酶(PHGDH)、序列相似性家族13 A(FAM11A)、磷酸丝氨酸转氨酶1 (PSAT1)、假尿苷酸合酶7(PUS7)、DEAD-box解旋酶1(DDX1)、溶质载体有机阴离子转运蛋白家族5A1(SLCO5A1)、Junctophilin 1(JPH1)、溶质载体家族16 1(SLC16A1)、MYCN Opposite Strand(MYCNOS)为神经母细胞瘤中MYCN扩增的共表达基因，与较差的预后相关，是神经母细胞瘤潜在的治疗靶点及预后指标。

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简介：Weinvestigatetheeffectsofspontaneouslygeneratedcoherence(SGC)onboththesteadyandtransientgainpropertiesinafour-levelinverted-Y-typeatomicsysteminthepresenceofaweakprobe,twostrongcoherentfields,andanincoherentpump.Forthesteadyprocess,wefindthattheinversionlessgainmainlyoriginsfromSGC.Inparticular,wecanmodulatetheinversionlessgainbychangingtherelativephasebetweenthetwofields.Moreover,theamplitudeofthegainpeakcanbeenhancedandtheadditionalgainpeakcanappearbychangingthedetuningofthecouplingfield.Asforthetransientprocess,thetransientgainpropertiescanalsobedramaticallyaffectedbytheSGC.ComparedtothecasewithoutSGC,thetransientgaincanbegreatlyenhancedwithcompletelyeliminatedtransientabsorptionbychoosingtheproperrelativephasebetweenthetwofields.Andtheinverted-Y-typesystemwithSGCcanbesimulatedinbothatomicandsemiconductorquantumwellsystemsavoidingtheconditionsofSGC.