简介：IsolationofHelicobacterpylori(HP)isthegoldstandardforthediagnosisofHPinfection.However,theconventionalisolationmethodistedious,expensiveandtroublesome,andisnotsuitableforuseingeneralpractice.Wehavedevelopedasimple,low-costandeffectivemethodfortheisolationofHP.TwobiopsyspecimensfromeachpatientwereinoculatedonaHPselectivebloodplate,andplacedinaspeciallydevisedplasticjarcoveredair-tightlywithalid.Afterfillingwithamixturegas(CO210%,O25%,N285%),

简介：AIM:Toinvestigatetheeffectsofthefreeradicalscavengerbis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate(IAC)inthedextransodiumsulphate(DSS)experimentalmodelofulcerativecolitis.METHODS:ColitiswasinducedinSpragueDawleymaleratsbyadministrationof5%DSSindrinkingwater.IAC(30mg/kg,lipophilicorhydrophilicform)wasadministereddaily(orallyorip)for6duntilsacrifice.Colonicdamagewasassessedbymeansofindirect(DiseaseActivityIndexscore)anddirectmeasures(macroscopicandmicroscopicscores)andmyeloperoxidase(MPO)activity.NeutrophilinfiltrationwithinthetissueandglutathioneS-transferaseactivitywerealsoinvestigated.RESULTS:DSS-inducedcolitisimpairedbodyweightgainandmarkedlyincreasedallinflammatoryparameters.Six-daytreatmentwithlipophilicIACsignificantlyreducedintestinaldamagecausedbyinflammation,inducedadown-regulationinMPOactivity(0.72±0.12and0.45±0.12withlipophilicIACpoandip,respectively,vs1.10±0.27inuntreatedDSScolitisanimals)andminimizedDSS-inducedneutrophilinfiltration,whilehydrophilicIACadministeredorallydidnotameliorateDSS-induceddamage.CONCLUSION:TheseresultssupportthehypothesisthatreactiveoxygenmetabolitescontributetoinflammationandthattheradicalscavengerIAChastherapeuticpotentialininflammatoryboweldisease.

简介：瞄准：低剂量的阿司匹林的使用很好被建立阻止心血管的疾病事件。然而，上面的胃肠的流血的发生和有它的使用的预言者(UGIB)是未知的。我们有希望地在低剂量的阿司匹林用户学习了消化剂溃疡的发生和结果。方法：有低剂量的阿司匹林上的冠的动脉疾病(CAD)的991个病人的一个总数是有希望地跟随起来的为出现和UGIB的首先就医的事件的临床的特征的二年了。结果：UGIB有一个双性人有45%发生在四月阿司匹林开始以内的形式的表示并且每年有1.5%的全面流行。没有UGIB相关的死亡。高血压(或=4.6，95%CI1.5-14.7，P=0.009)，消化剂溃疡的历史(或=3.1，95%CI1.1-9.0，P=0.039)，第三级的教育(或=3.08，95%CI1.1-9.0，P=0.039)并且更高瘦的身体质量(P=0.016)独立因素与UGIB被联系。硝酸盐的使用没减少UGIB。结论：在有长期的低剂量的阿司匹林上的CAD的病人的UGIB的发生是低的，但是伴有重要病态。与阿司匹林的延长使用，UGIB继续是为有风险因素并且特别在有消化性溃疡，UGIB在趋于在阿司匹林治疗以后早发生的历史的病人的那些的一个问题。