简介:IthasbeenshownthatIL-2andIL-15canhaveopposingeffectsonlifeanddeathofTcells.However,theroleofIL-2andIL-15inregulatingthefateofothercelltypesislessclear.Inthepresentstudy,weexaminedtheimpactofIL-2andIL-15onlifeanddeathofpre-BcellsusingtheBAF-B03line.WeshowedthatBAF-B03cellsconstitutivelyexpressedtheprivateIL-2RαchainandIL-15Rαchain,andthesharedIL-2Rβchainandγcchain.StimulationofBAF-B03cellsinvitrowithIL-2andIL-15inducedvigorouscellproliferationinadose-dependentfashion.TitrationofIL-2andIL-15intheassayshowedthatthemitoticeffectsofIL-2andIL-15wereremarkablysimilar,Howerer,thesensitivitiesofBAF-B03cellstoFasmediatedapoptosisafterIL-2andIL-15stimulationwerestrikinglydifferent.CellsculturedinIL-2readilyunderwentapoptoticcelldeathuponcross-linkingoftheFasreceptorwhereascellsculturedinIL-15wereextremelyresistanttoFastriggeredcelldeath.Theanti-apoptoticeffectofIL-15inthismodelwasassociatedwithincreasedexpressionofBcl-xL.FLIPexpression,however,wascomparablebetweenIL-2andIL-15stimulatedcells.WeconcludethatIL-2andIL-15havediametricallyoppositeeffectonthefateofBAF-B03cells,althoughbothcytokinessharesimilarreceptorstructureandexhibitsimilarmitoticactivities.
简介:摘要目的探讨IL-4,IL-12在宫颈病变发生发展中的作用以及对机体免疫功能的影响。方法应用RT-PCR技术检测IL-4mRNA,IL-12p35以及IL-12p40mRNA在正常宫颈组、CINⅠ、CINII-III和宫颈癌组中的表达。结果在宫颈癌组中IL-4表达高于CIN和正常宫颈组,CINII-III组高于CINⅠ和正常宫颈组(P<0.05),IL-12p35和IL-12p40mRNA表达均低于CIN和正常宫颈组,CIN各组均低于正常宫颈组(P<0.05)。结论体内IL-4,IL-12水平与肿瘤的发生密切相关,体内IL-12降低和(或)IL-4升高可促进宫颈癌的发生发展。
简介:Objective:ToinvestigatetheexpressionofHPV16mRNAinnormalhumankeratinocytestransfectedwithpSV2-neo/16.Firsthumankeratinocyteswereculturedintheserum-freemediumM154.Second,theplasmidpSV2-neo/16wastransfectedintothehumankeratinocytesusingatransfectingreagent.Third,RT-PCRandSouthernBlottingwereusedtodetecttheexpressionofHPV16mRNAandDNAinthetransfectedkeratinocytes,respectively.Results:TheexpressionofHPV16mRNAwassuccessfullyamplifiedandan110bpwasditectedbyRT-PCR.A7.9kbfragmentwasconfirmedinthetransfectedkeratinocytesbySouthernBlotanalysis.Conclusion:HPV16mRNAandDNAweresuccessfullydetectedinthehumankeratinocytes.
简介:细胞因子(cytokine)是具有多种生物活性的可溶性小分子蛋白质,包括白细胞介素(interleukin,IL)、趋化因子(chemokine)、黏附分子(adhesionmolecule)、肿瘤坏死因子(tumornecrosisfactor,TNF)和干扰素(interferon,IFN)等.白细胞介素是一组由单个核细胞(包括单核-巨噬细胞和淋巴细胞)产生的细胞因子,作用于淋巴细胞、巨噬细胞和其他细胞.脑缺血发生时,一些细胞因子参与了脑缺血后炎症反应的启动、发展过程.本课题采用尸检的方法,取因脑梗死死亡患者脑组织以对侧正常脑组织为对照,通过免疫组化检测IL-17、TNF-α和IL-1β在人类缺血脑组织中表达状况.
简介:Interleukin12(IL-12)and/orinterleukin18(IL-18)geneablatedmicewereappliedfortheinvestigationofthetissueexpressionofinterferonγ(IFN-γ).ForIL-12^-/-,IL-18^-/-,IL-12^-/-/18^-/-andwtmice,reproductiveperformancewererecordedandIFN-γconcentrationsinheart,lung,liver,spleen,kidneyandserumwerequantifiedbyELISA.TherewerenosignificantdifferencesofIFN-γ,inheart,lungandkidneybetween4strainsalthoughcontrolgroupwashigher.ItwasobservedthatforIL-12^-/-mice,comparedwithother3groups,IFN-γinliverandspleenweredecreased(p<0.05)andreproductiveperformanceappearedtobeimpaired.SerumIFN-γlevelofIL-12^-/-/18^-/-micewassignificantlyhigher(p<0.05).ItwasshowedthatIFN-γproductionsunderthenormalconditionwereindependentuponIL-12andIL-18,itsexpressionsinvarioustissuesweredifferent,andoptimalIFN-γisnecessaryforthenormalgrowthanddevelopmentofmammals.Thisstudyishelpfulforclinicalcytokinestherapy.Cellular&MolecularImmunology.2005;2(1):68-72.
简介:目的探讨细胞因子IL-2和IL-10在早期梅毒发病中的作用。方法采用ABC-ELISA技术检测48例早期梅毒患者血清IL-2和IL-10水平。结果(1)早期梅毒患者血清IL-2水平明显高于正常对照组,IL-10水平明显低于正常对照组(P值均<0.001)。(2)一期梅毒患者血清IL-2和IL-10水平与二期梅毒无明显差异(P值均>0.05),不同RPR滴度间IL-2和IL-10水平无明显差异(P值均>0.05)。(3)治疗后IL-2水平明显下降(P<0.05),IL-10水平上升不明显(P>0.05)。(4)IL-2和IL-10之间呈正相关(r=0.5385,P<0.05)。结论早期梅毒出现Thl上调,在抵御TP感染过程中发挥积极作用。