简介：ThebindingofCo(bpy)2dppz3+tocalfthymusDNAwasinvestigatedbyusingabsorptionandemissionspectroscopy,DNAmeltingtechniques,cyclicvoltammetry,viscosityandelectro-phoresismeasurements,wherebpyis2,2’-bipyridyl,dppzisdipyrido[3,2-o:2’,3’-c]phenazine.Thebindingcompoundshowsabsorptionhypochromicity,fluorescenceenhancement,andincreasingofDNAmeltingtemperatureandthespecificviscosity.CVmeasurementshowstheshiftsinoxidation-reductionpotentialandchangeinpeakcurrentwithadditionofDNA.ThecompoundisalsoshowntobemoreefficientphotosensitisersforstrandbreaksinplasmidDNA.

简介：TheeffectofCdionsonsalmonspermDNAwasstudiedbymeansofcirculardichroism(CD),Ramanspectroscopy,X-rayphotoelectronspectroscopy(XPS)andfluorescencespectroscopy.TheCDspectralandfluorescentprobe-acriflavineresultsindicatethattheDNAunderwentaconformationchangeupontheadditionofCdions.XPSandRamanstudiesrevealthatthereexistedinteractionsbetweenCdionsandthephosphategroupsoftheDNA.Inaddition,anewbandappearedat803cm-1intheRamanspectra,whichcanbeattributedtocharacterizing"marker"bandofA-DNA.ItisconcludedthatCdionscanbecoordinatedbythephosphategroupsoftheDNAandinducetheconformationchangesoftheDNAfromB-DNAtoA-DNA.

简介：HydrolysisofDNAisanimportantenzymaticreaction,butitisexceedinglydifficulttomimicinthelaboratorybecauseofthestabilityofhydrolysisofDNA.Inthispaper,thecleavageactivityofcomplexesformedbetweenCu(Ⅱ)andfourdifferentaminoacidoraminoacidmethylesteronDNAisstudiedbygelelec-trophoresis.ItisfoundthatDNAcouldbecleavedbyCu(Ⅱ)-L-HisandCu(Ⅱ)-L-Hismethylestercomplexesandtheefficiencyofcleavageislargelydependentonthemetalion-to-ligandratio.FurtherexperimentsshowthatthecleavageofDNAmediatedbyCu(Ⅱ)-L-HiscomplexesoccursviaahydrolyticmechanismandtheactivechemicalspeciesthataffectsDNAcleavageisproposedtobeMI2H+andML2H22+.

简介：Adetectionofanthracyclineantitumordrugdaunomycin(DNR)reactingwithDNAinsimulatemetabolisminvitrohasbeenmade.ItwasfoundthatDNRcouldreactwithDNAtoformDNR-DNAadducts.TheadductcompositionsofDNRwithfishspermDNAandthermallydenaturatedDNAweredetermined.TheequilibriumassociationconstantKofDNRwithfishspermDNAis1.98×10^5L/molandthatofDNRwithdenaturatedDNAis2.29×10^4L/mol.Semiquinonefreeradicals,metabolicproductsofDNR,candestroybothfishspermDNAanditsthermallydenaturatedDNA.ItisverifiedbyhyperchromiceffectincreaseobservedinUVspectrumandAFMexperiments.ThemechanismofDNAdegradationhasalsobeeninvestigated.Resultsobtainedallowonetoexplainthereasonofsideeffectofanthracyclinedrugandgivethewaytodepress,whichwereofclinicalsignificance.

简介：AvohammetricstudyoftheinteractionofneutralRed(NR)withDNAatagoldelectrodeinaphosphatebuffersolutionisdescribed.AfteraddingDNAinanNRsolution,thereductionpeakcurrentofNRdecreases.ThebindingmeehahismsofNRtoDNAindifferentpHrangesaredifferent.ThereductionpeakpotentialofNRinapH7.0phosphatebuffersolutioninthepresenceofDNAshiftspositively,indicatingthatthebindingofNRtoDNAisintercalationaction,butatpH=6.0thereductionpeakpotentialofNRshiftsnegatively,indicatingthatthebindingofNRtoDNAiselectrostaticaction.TheformedcomplexesareDNA-NRwhen[NR]/[DNA]<0.18andDNA-3NRwhen[NR]/[DNA]>0.35,respectively.

简介：MtDNAwassuccessfullyextractedfromtenindividualbones(femurs)inthetombsofancientJushiinTurfanbasin,datedbacktotheyearabout3000-2500yearsago.Bymeansoffouroverlappingprimers,wegotnucleotidesequenceofthe218bplength.AncientmtDNAwasanalyzedbythesequencingofhypervariableregionⅠofthemtDNAcontrolregion.Theresultshowsthat9haplotypeswith24polymorphicsiteswereobtained.ThephylogeneticanalysisindicatedthatMongoliansandAltaiarethepopulationgeneticallyclosesttotheJushigroupsandJushimtDNApoolbeinganadmixtureofeasternAsianandEuropeanlineages.SoourpreliminarydataimplythatanancientminglingofEuro-AsianpopulationhadexistedinTurfanbasinpriortotheearlyIronAge.

简介：Rheumatoidfactors(RFs)arethecharacteristicautoantibodiesofrheumatoidarthritis.Recentresearchesinourlaboratoryshowedthattheimmobilizedsingle-strandedDNA(ss-DNA)immunoadsorbentcanselectivelyremoveRFsfromtheserumofpatients.Inthepresentpaperarestudiedthemodificationofargininine,tryptophan,lysineresiduesandcarboxylterminusofIgGRF,whichwasseparatedfrompatients′serum,with1,2-cyclohexanedione(CHD),N-bromosuccinimide(NBS),pyridoxal5′-phosphate(PP)and1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC)respectively,andtheireffectsontheadsorptioncapacityoftheimmobilizedss-DNAimmunoadsorbentforIgGRF.Afterthespecificmodification,thecorrespondingadsorptioncapacitiesoftheadsorbentswerechangedfrom48%,46%,44%and54%to84%,14%,21%and81%,respectively.Theseresultsindicatethattheelectrostaticorionic-bondingisessentialfortheinteractionbetweenss-DNAandIgGRF.

简介：TheroleplayedbyDNAinmolecularbiologyisclearlyrecognizedtobevitaltolifeonthisplanet.8-oxo-7,8-dihydro-2deoxyguanosine(=8-OHdG),isprobablythemostimportantproductof"oxidativestress”inDNA.ItsconcentrationinDNAis,infact.aquantitativeanalysisofthedegreeofDNAdamagethatanorganismhasundergone.Duetotheimportanceof8-OHdGofnucleicacidginmutagenesis,carcinogenesisandaging,numerouschemicalandbiologicalinvestigationshavebeenmadeonthissubjectinthepasttime.Kuchinoandco-workershavefoundthat8-OHdGresidueinDNAismisreadingduringtheprocessofDNAreplication.Recently,somereportshavebeenpresentedonhigh8-OHdGlevelsinpatientssufferingfromvariousdiseasessuchaschronichepatitis,Fanconisanemia,diabetesmellitusandHelicobacterpyloriinfections.Asaresult,8-OHdGisausefulmarkerforthestudyofDNAdamagearisingfromreactiveoxygenspeciesandisofgreatsignificanceforcancerresearch.The8-OHdGlevelsinDNAcanhelpunderstandthemechanismofcarcinogensandleadtomoreeffectivetreatmentsformanydifferenttypesofcancer.Forthesereasons,ananalysisof8-OHdGwithquickness,lowcostandhighaccuracyisrequired.

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简介：Thedye-dopedsilicananoparticlescanbeusedasnanobiosensorsthatareabletorecognizeanddetectspecificDNAsequence.Inthispaper,sphericalnanosizedluminol/SiO2compositeparticleshavebeensynthesizedwithreversemicellsviahydrolysisoftetraethylorthosilicate(TEOS)inthemicroemulsion.ThenanoparticlesweremodifiedwithchitosanandusedtolabelDNA,formingtheDNAprobewhichwasusedtohybridizewithtargetDNAimmobilizedonaPPymodifiedPtelectrode.Thehybridizationeventswereevaluatedbyelectrogeneratedchemiluminescence(ECL)measurementsandonlythecomplementarysequencecouldformadouble-strandedDNA(dsDNA)withDNAprobeandgivestrongECLsignals.Athreebasemismatchsequenceandanon-complementarysequencehadalmostnegligibleresponses.Duetothelargenumberofluminolmoleculesinsidesilicananoparticles,theassayallowsdetectionatlevelsaslowas2.0×10^-12mol/LofthetargetDNA.TheintensityofECLwaslinearlyrelatedtotheconcentrationofthecomplementarysequenceintherangeof5.0×10^-12—1.0×10^-9mol/L.

简介：Hereinwedemonstratetheconstructionofthreetypesofparallelgoldnanorod(AuNR)clustersusingaDNAorigamirod(DOR)asthetemplate.BasedontheprecisecontroloverthepositionofcapturestrandsonDOR,numberandorientationoftheAuNRclusterscanbewellengineered,asevidencedbybiologicaltransmissionelectronmicroscope(TEM).Importantly,theAuNRclustersexhibitchiropticalresponseswhicharestronglyaffectedbythenumberofAuNRonrod-likeDNAorigami.

简介：新奇建筑群[公司(phen)2HPlP]Cl3[phen=phenanethroline，HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][l，10]phenanethroline]被元素的分析综合了，在结构上描绘了，紫外，红外和1HNMR光谱学。有小牛胸腺DNA(CTDNA)的建筑群的相互作用被学习了使用吸收和排放光谱学，DNA融化技术和周期的voltammetry。混合物看吸收hypochromicity，荧光改进和DNA融化温度增长是否有约束力到CTDNA。CV测量与DNA的增加在山峰电流在减小潜力和一个变化显示出移动。这些结果证明混合物插入到DNA基础对的地席。山峰潜力的移动显示在建筑群和DNA之间的离子相互作用模式。到DNA的混合物的有约束力的常数是4.37灳慬瑮?郥誸???蚰铧?髧??螻?趤??藥???藥薗郧芀漀椭吗？？

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简介：ＳＴＵＤＹ　ＯＮ　ＴＨＥ　ＲＥＬＡＴＩＯＮＳＨＩＰ　ＢＥＴＷＥＥＮ　ＭＥＬＴＩＮＧ　ＰＯＩＮＴ　ＡＮＤ　ＡＣＩＤ　ＤＥＮＡＴＵＲＩＮＧ　ＡＣＩＤＩＴＹ　ＯＦ　ＤＮＡ　ＢＹ　ＵＬＴＲＡＶＩＯＬＥＴ　ＳＰＥＣＴＲＯＭＥＴＥＲＳＴＵＤＹＯＮＴＨＥＲＥＬＡＴ...

简介：采用荧光光谱法和紫外-可见分光光谱法,在生理条件下(含0.1mol/LNaCl的Tris-HCl缓冲溶液,pH=7.40)研究了紫杉醇与生物大分子DNA的相互作用方式.结果表明DNA引起紫杉醇的紫外吸收光谱明显的红移和增色,并导致紫杉醇的荧光猝灭;测定不同温度下紫杉醇与DNA的荧光动态猝灭常数,结果显示K27℃=10266,K37℃=8755,K47℃=5788,即K27℃>K37℃>K47℃,说明动态猝灭常数随着温度升高而减小.同时考察了离子强度、磷酸根浓度及猝灭剂KI对紫杉醇与DNA相互作用的荧光强度的影响,三者对紫杉醇与DNA相互作用都无明显影响.表明DNA对紫杉醇的荧光猝灭类型是静态猝灭,紫杉醇与DNA的相互作用方式是嵌插作用.

简介：Thelackofefficientandnon-toxicgenedelivery,preferablywithnon-viralDNAvectors,isgenerallyregardedasamajorlimitationforgenetherapy.Inthisstudy,awheathistoneH4genewasclonedfromTriticumaestivum,sequenced,modifiedandexpressedinE.coli.ThewheathistoneH4geneandreconstructedH4TLgeneencodedwheathistoneH4andarecombinantproteinof141aminoacidswithanapproximatemolecularweightof15500.GelelectrophoresismobilityshiftassaysdemonstratedthatthepurifiedproteinhadhighaffinityforDNA.Mostsignificantly,thecomplexofplasmidpEGFP/C1withH4TLwastransfectedwithincreasedefficiencyintoMCF-7,HO8910,LNCap,A549andHeLacellsinvitro.Theseresultsdemonstratethatthetargetingofnon-viralvectorstotumor-specificreceptorsprovidesacheap,simpleandhighlyefficienttoolforgenedelivery.

简介：用溴化乙锭（EB）作为荧光探针研究了各种茶叶多糖、多酚类化合物对DNA的保护作用.测定了龙井、普耳茶、乌龙茶等8种茶叶中多糖和多酚类提取物存在下DNA与EB混合液的荧光积分强度,并把不同茶叶与DNA相互作用的常数定义为结合常数D,根据D的大小讨论了多糖及多酚类化合物的存在DNA保护作用的影响.结果表明,8种茶叶与DNA有较好的作用,但作用程度不同.D值越大,茶叶对DNA的保护作用越好.多糖保护作用顺序如下：祁门红茶〉绿茶（安徽）〉普尔茶〉乌龙茶〉福建政和红茶〉寿宁绿茶〉信阳毛尖〉龙井;多酚保护作用顺序如下：祁门红茶〉福建政和红茶〉寿宁绿茶〉龙井〉乌龙茶〉绿茶（安徽）〉信阳毛尖〉普尔茶.

简介：N复杂的Cu2+的相互作用，有DNA的N二度(2-aminoethyl)-2,6-pyridinedicarboxamide(BAP)被agarose胶化电气泳动分析学习。结果显示BAP-Cu2+建筑群能以生理的状况支持supercoiledDNA的phosphodiester债券的劈开，哪个是3.2畤瑣潩?牯杩湩漠?敧杯慲桰捩漠楲楧?景眠湩獥吗？？