简介:Tosupportthescientificbasisforrapididentificationofpathogenicbacteriaandotherstud-ies,thesequencesofhsp60geneinmajor34speciesof16genusofpathogenicbacteriaweresearchoutinGenBankandaproperpairofuniversaldegenerateprimerwasdesignedbymeansofthemolecu-larbiologicalsoftwawePrimer5.0andOligo6.0.ThisprimerwasthenusedinthePCRamplification,andthehsp60genefragmentsoftheselectedpathogenicbacteriacouldbeamplifiedusingthisdegener-ateprimer.Bywayofbioinformationalanalysis,theconservation,variationandtheinterspeciesphylo-geneticrelationsofthehsp60genesequencewereanalysed.Fromtheresultsofthecomparativestudyonsequences,itwasdemonstratedthatthehsp60genewascharacterizedbyconservationandvaria-tion,inwhichtheconservedandmutantregionsco-existedandseparatelydistributedwithmanysmallmutantregionsdistributedamongtheconservedregions,justlikethemosaic.Thephylogenetictreeamongdifferentpathogenicbacteriadrawnfromthehsp60geneanalysiswasprovedtobeconsistentwiththosefrom16SrRNAand23SrRNA.Itisconcludedthatthesequencedistributionofhsp60genewouldprovideasolidbasisfortherapididentificationofpathogenicbacteriaandthedevelopmentofadiagnosticmicroarray.
简介:IntroductionTherecentdiscoveryofaclassofcarboncontainingmoleculesknownasthefulereneshasproducedaseriesofmaterialswithawideran...
简介:TheinteractionbetweenC60’sinsolidC60hasbeencalculatedby(exp-6-1)potential,andthecauseandthecontrolledfactorofthehighrapidrotationsofC60’swerediscussed.InordertodescribethedisordereddegreeofC60rotation,anequivalentMisintroduced.Thephasetransitionsatthe~260Kandatthe~90KarestudiedfromtheviewpointofC60rotation.Thepotentialbarriersoftheorderedrotationbelowthe~260Kandthedisorderedrotationabovethe~260Khavebeengiven,andtheeffectoftheexternalpressureonthetemperatureofphasetransitionhasalsobeengiven.
简介:目的:探讨HSP60(热休克蛋白60)在大鼠急性青光眼模型视网膜组织中的表达及其与血清中相应抗体的关系。方法:将SD大鼠70只随机分为高眼压组60只,正常对照组10只。大鼠全身及表面麻醉后,将一盛有等渗的生理盐水的储容器相连的7号针头于3:00位的角膜缘处刺入前房,提升储容器的高度,使眼内压达到110mmHg,但不超过150mmHg,观察大鼠眼前段变白,且视网膜色白,未见红色反光时即为视网膜缺血,缺血1h后再灌注,并于再灌注后2,6,12,24,72,168h将大鼠麻醉过量致死,处死前测眼压,抽血2mL,供酶联免疫吸附测定使用,分析视网膜组织内HSP60抗原产生的血清中相应抗体水平。并立即取出眼球,同时对鼠眼视网膜组织进行石蜡切片,采用免疫组化法检测视网膜组织中HSP60的表达及分布情况,并对检测结果进行统计学分析。结果:急性高眼压诱导的视网膜缺血/再灌注组眼压明显升高,视网膜组织中的HSP60阳性表达率在术后各时间点,高眼压组与正常对照组比较,差异有统计学意义(F=97.21,40.72,83.85,95.82,48.63及44.37,均P〈0.01)。神经节细胞(retinalganglioncell,RGC)中HSP60阳性表达随着眼压升高及高眼压持续时间延长逐渐增强,且视网膜神经纤维层中也出现较明显的HSP60阳性表达。结论:HSP60表达增强可能在急性高眼压所致的视神经病变中具有重要作用。
简介:背景:血清ESM-1和HSP60水平对结直肠癌(CRC)的诊断价值国内外已见报道,但两者联合检测的应用价值尚无相关研究。目的:探讨血清ESM-1和HSP60对CRC的诊断效能。方法:选取2017年10月—2018年3月唐山市人民医院的CRC住院患者124例、健康体检者48例。以ELISA法测定ESM-1和HSP60含量,评估两者诊断CRC的价值,并探讨联合ESM-1与HSP60对CRC的诊断效能。结果:CRC组血清ESM-1和HSP60含量均显著高于正常对照组(P<0.001)。CRC组血清ESM-1和HSP60含量与TNM分期相关(P<0.05),而与性别、年龄、病变部位无关(P>0.05)。ESM-1、HSP60诊断CRC的ROC曲线下面积(AUC)分别为0.865、0.912,敏感性分别为93.55%、83.87%,特异性为72.92%、81.25%。ESM-1与HSP60并联实验的敏感性和特异性分别为98.96%、59.25%,串联实验分别为78.46%、94.92%,两组AUC相比无明显差异(0.892对0.904,P>0.05)。结论:ESM-1和HSP60诊断CRC均具有较高的应用价值,两者并联可作为CRC的早期筛查方案,串联实验适用于CRC的辅助诊断。
简介:TheconstructionofadsorptivetypeC60modifiedelectrodeanditselectrochemicalreductionandoxidationbehaviorinaqueoussolutionsaredescribedinthisreport.Fourpairsofone-electrontransferreductionandoxidationcyclicvoltammetryisobtainedinaqueoussolutioncontaining30%CH3CNand2%(C2H5)4NOH.ItisreportedinthispaperthattheC60modifiedelectrodealsocatalyzestheelectrochemicalreductionofdissolvedoxygenin40%DMFand2%(C2H5)4NOHaqueoussolutionandthismightopenanewfieldforthepotentialapplicationsofC60inelectrochemistryandelectroaualyticalchemistry.
简介:在主人寄生虫相互作用的热吃惊蛋白质(hsps)的角色上的抽象很分子的工作集中了于脊椎动物,而非无脊椎动物。这里,互补DNA(cDNA)三hsp基因(hsp20,hsp75和hsp90)定序的完整的长度被endoparasitic黄蜂Pteromaluspuparum从Pierisrapae,和他们的transcriptional应答放大到parasitization被调查。hsp20,hsp75和hsp90的cDNA顺序分析揭示了531的开的读物框架,在长度的2328和2157bp,它编码蛋白质与分别地计算了19.5,75.48和82.7kDa的分子的重量。氨基酸序列的比较显示出那P。rapaehsp20分享了高度分叉的相同到另外的昆虫的,当hsp75和hsp90显示出高相同到他们另外的种类的对应物时。表示分析显示这三基因被P响应parasitization影响。puparum。在寄生于的蛹的hsp20抄本与非寄生于的蛹相比是更高的。hsp75和hsp90的表示是下面调整的跟随parasitization。结果显示hsps涉及host-parasitoid相互作用。