简介：BACKGROUND:Presently,over40kindsofduralgraftshavebeensuccessivelyusedinclinic.Amongthem,lyophilizedhumanduramaterwithgoodhistocompatibilityandlesscomplicationsisappliedmostwidely.Butthereareafewreportsoncasesofinfectedspongiformencephalopathyfollowingapplicationoflyodura.Moreidealrepairmaterialsdeservetobefurtherinvestigated.OBJECTIVE:Toinvestigatetheefficiencyandsafetyofbiologicalduralgraftmadebymeningesfromporkerstorepairmeningealinjury.DESIGN:Aself-controlobservation.SETTING:WuhanGeneralHospitalofGuangzhouMilitaryAreaCommandofChinesePLA.MATERIALS:SixteenNewZealandRabbits,ofeithergender,weighingfrom2to3kg,ofcleangradeⅡ,withtheageof0.5–1year,wereinvolvedinthisexperiment.TheinvolvedrabbitswereprovidedbytheAnimalExperimentalCenteroftheFirstMilitaryMedicalUniversityofChinesePLA.Biologicalsurgicalpatch(duralgraft)wasdevelopedbyGuangdongGuanhaoBiotechnologicalCo.,Ltd.Itwasprocessedbyusingmeningesfromporkersbytissueengineeringtechnology.METHODS:ThisexperimentwascarriedoutintheExperimentalCenterofthe157HospitalofChinesePLAbetweenDecember2003andJune2004.①Theexperimentalrabbitswereanesthetized.Duramaterwasexposedfromtwosidesofpostmediallineofcoronalsuture.Arectangularduramaterabout8mm×8mminsizewascutoff.Thenabiologicalsurgicalpatch(duralgraft)wasshearedintoinsertwith8mmdiameterandsutured.Theleftduramaterwasuntouchedandusedascontrol.Scalpwassutured,andpostoperativewoundhealingandrecoverywereobserved.②Theanesthetizedrabbitsweresacrificedatpostoperative3,14,30and90days,4rabbitsonce.Thewholeheadwascutoff,anditsscalpwasremoved.Afterwards,theheadwasfixedbyformalin.Tissuesinoperativesitewereobtained,performedroutineparaffinembedding,slicedandconductedHEstaining,finally,thesectionswereobserved.Whitebloodcellsinv

简介：Themechanismsfortheregulationofsynapticdopamine(DA)includeitsreleasefrompresynapticvesicles,itsinteractionwithpost-synapticandpre-synapticDAreceptors,thereuptakeofDA,viadopaminetransporter(DAT),thediffusionofDAanditsmetabolismbymono-amineoxidase(MAO)andcatechol-O-methyltransferase(COMT).DAcontrolscomplexand