简介：Withaself-madenear-infraredanalyticalinstrumenttobloodandoxygenparametersinhumanbrain,80casesinwhich20arehealthypersonsand30areanaesthetisedcasesandothersarepatientswithheartfunctionlackistakentoexamine,andthedataofbloodandoxygeninbraintissuewerecollectedandanalyzedbythemethodofpowerspectrumandcorrelationfunction.Theresultsindicatethat:(1)Theaveragebrainoxygensaturationofhealthypersonsandanaesthetisedcasesisabout80%,inaccordwithnormalparameterofphysiology.Contrastively,theaveragebrainoxygensaturationofpatientswithheartfunctionlackis72.8%,whichisobviouslylessthanthatofhealthypersonsandanaesthetisedcases.Theprobabilityofmedicalstatisticsislessthan0.01.(2)Theshapesofwaveofbrainbloodandoxygenforthehealthypersonandtheanaesthetisedcaserevealsmallperiodicalfluctuationswithstableshapeandbaseline,andthetrendofincreaseordecreaseofbloodandoxygenparametersinbraintissueissynchronousandaphasereversal,butforthepatientwithheartfunctionlackinabrainoxygenlackstate,theshapesofwaveareirregular.Thisisahintthatnearinfraredlightpassingthroughtissuecanreflecttheintuitionisticchangeofbrainbloodandoxygenparameters.(3)Thepowerspectraofbrainbloodandoxygenforthehealthypersonandtheanaesthetisedcasehasaclearmainpeak,narrowbandwidthandperfectsuperpositioneachother,butthepowerspectraforthepatientwithheartfunctionlackinabrainoxygenlackstateisonthecontrary.(4)Theaveragecrosscorrelationcoefficientofbrainbloodandoxygenforhealthypersonsandanaesthetisedcasesis-0.9825±0.1027closeto-1.Buttheaveragecrosscorrelationcoefficientforpatientswithheartfunctionlackinabrainoxygenlackstateismerely-0.8923±0.1035whichisobviouslygreaterthan-1andtheprobabilityofmedicalstatisticsislessthan0.01.Theclinicexperimentshaveprovedthattheshapes

简介：Anon-equilibriumchromatographicratemodelwasemployedtosimulatetheaffinitychromatographyofurokinase.Thechromatographyprocesswasdevelopedtoayieldofhighpurityproductofurokinasefromcrudematerials.Theaffinitygelusedintheprocesswaspreparedbyanepichlorohydrin-activationmethodusingepichlorohydrinactivatedSepharose4Basamatrixandp-aminobenzamidineasaligand.ThechromatographicprocesswerenumericallysimulatedandanalyzedwiththeaidofVERSE-LCcomputersimulator.Consideringthebasicprinciples,ratemodelwiththebackmixingincolumninletwasutilizedinsimulatingandstudyingtheeffectofthecolumninletpatternonotherparameters.Comparisonofthesimulationresultswiththeexperimentaldatashowedthattheratemodelcanbeusedtodescribetheaffinitychromatographyofurokinaseinafixedbedcolumnwithsatisfactoryaccuracy.

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