简介:WeestablishasystemtomeasurethefunctionalabsorptioncrosssectionofphotosystemII(PSII)(σPSII)andmaximumquantumyieldofphotochemistryinPSII(Fv/Fm).Thesystemutilizesasequenceofhighfrequencyexcitationflashesatmicrosecondintervalstoinduceamicrosecond-levelfluorescenceyieldcurve.ParametersσPSIIandFv/Fmarecalculatedbyfittingthecurveusingnonlinearregression.Experimentalresultsshowthattherelativestandarddeviation(RSD)ofthesystemislessthan3%,andthecorrelationcoefficientofFv/Fmvaluesmeasuredbythissystemandthosemeasuredbypulseamplitudemodulationmethodis0.950.
简介:Influencesofthescatteringphasefunctionsonspatiallyresolveddiffusereflectancefromahomogenoussemi-infinitemediumclosetosourcearestudiedwithMonteCarlosimulation.Itisshownthattheinfluencesofopticalparametershigherthanthesecondorderonthediffusereflectancearequiteweakintheregionfrom0.3toseveraltransportmeanfreepatheswhenHenyey-Greensteinphasefunctionoracombinedphasefunctionoftwoparametersareused.ButthisinfluencemaybesubstantialifthedoubleHenyey-Greensteinfunctionisusedtodescribethescatteringpropertyoftissue.