简介:Afragmentwithabout798bpofcabgenewasamplifiedfromthefirststrandofMoso(Phyllostachysedulis)cDNAthroughRT-PCRmethod,namedascab-PhE4(cabgene4fromPh.edulis).Thecab-PhE4(GenBankaccessionnumber:EF405878)geneencodes265aminoacid.Thebioinformaticsanalysisindicatedthattheproteinencodedbycab-PhE4hasachlorophylla/b-bindingdomain(64th-232ndposition),aproteinkinaseCphosphorylationsite(33rd-35thposition),aN-myristoylationsite(169th-174thposition),andaninvolucrinrepeat(207th-216thposition).Theaminoacidsequenceofcab-PhE4showedhighsimilaritywiththecabgenesofZeamays,Triticumaestivum,Musaacuminata,PanaxginsengandOryzasativa,morethan90%,respectively.
简介:BydeterminationofthechangeofendogenoushormoneZr,iPA,GA3,IAAandABAduringdifferentflowerbuddifferentiationstagesofPhyllostachyspraecox,whichisidentifiedthroughbothfieldobservationandlabanalysis,andwiththereferencetothepreviousresearchachievementsonbambooflowering,thefloweringmechanismassumptionofPhyto-HormoneRegulationandGeneActivationofPh.praecoxisinducedinthisarticle:Bambooflowerbuddifferentiationcanbedividedinto3stages,i.e.flowerbudinduction,flowerbudinitiationandflowerbuddevelopment;Bambooleavessenseandreceivefloweringsignalsfromenvironmentstochangeitshormonelevel,esp.ratiosofiPA/ABAandiPA/GA3;FloweringgeneisactivatedoncetheratiosofiPA/ABAandiPA/GA3reachaproperthreshold,anditproducesDNAandRNAcarryingfloweringcodeandtransportsthemtotoporsidebudsnearby,andthenproteinnecessaryforflowerbuddifferentiationcomesout,asaresultofwhichtheflowerbudinductionistriggedandstarted,followedbyflowerbudinitiationanddevelopment.Intheinductionstage,ratioofC/Nisnearlyconstant,butincreasesintheinitiationstage.ThereforeitclarifiesthattherisingofC/Nratiodoesnotbringaboutbamboofloweringinitially,anditisafollow-upreactionsofprocessinitiationofbambooflowering.Itprovesthatbamboorhizomeisdirectlyinvolvedintheflowerbuddifferentiation.Thisassumptioncanwellexplainmysteriousphenomenaofbambooflowering,andbyintegratingthecurrentseveralassumptions,answerthedifficultandperplexingquestionsregardingbamboofloweringwhichhavenotbeenansweredbythepresentassumptions.
简介:Theproteinstructureofthecellulosesynthase-likeprotein(CSL)wassimilartocellulosesynthase(CesA),includingtheconservativesequenceD,D,D,QXXRW.Onefull-lengthcDNAofthecellulosesynthase-likeproteinD(CslD)genewasclonedbyreversetranscriptase(RT)-polymerasechainreaction(PCR)with5’,3’rapidamplificationofcDNAends(RACE)methodsusingdegenerateprimersdesignedfromthehomologoussequencesoftheCesAgenes.Amultiplecomparisonsequenceanalysiswasconductedconcurrentlywithbioinformaticmethodstoanalyzetheobtainedsequence.ResultsofthesequenceanalysisshowedthatthiscDNAwas4150bpinlengthandcontainedasingleopenreadingframeencodingaproteinof1132aminoacids.Themultiplecomparisonsequenceanalysisshowedthatthededucedaminoacidsequencesharedhighsimilarity(over71%)withtheClCslDgenesfromPopulustremuloides,Oryzasativa,andArabidopsisthaliana.Thisworkwillhelplayanimportantfoundationforfurthermolecularstudieswithcellulosesynthesisofplants.
简介:白桦作为重要的制浆造纸树种,研究其不同种源纤维素和木质素含量的变异在纸浆材造林选择上具有十分重要的意义。本研究选择帽儿山地区实验林内17个白桦种源10a生试材,通过测定其纤维素和木质素的含量,结合方差分析和多重比较,研究其纤维素和木质素的种源差异,得出结论如下:白桦17个种源10年生试材纤维素含量的变异幅度在39.92%~51.14%,平均值是45.53%,其中绰儿种源10年生纤维素含量最高,为51.14%,青海种源最小,为39.92%;白桦17个种源10年生试材木质素含量的变异幅度在12.99%~28.67%,平均值是21.73%,其中辉南种源10年生木质素含量最高,为28.67%,新疆种源最小,为12.99%。方差分析结果显示白桦纤维素含量、木质素含量的差异在种源间达到极显著水平。多重比较的结果显示,在选择高纤维素用材时,首选绰儿种源,其次为东方红种源,清源种源;在选择高木质素用材时,首选辉南种源,其次为清源种源,乌伊岭种源。
简介:总结引种洛阳后萌发较晚的紫斑牡丹茎叶生长规律情况,结果表明,萌发期为3月上旬;7个品种紫斑牡丹的茎高、叶长和叶宽年平均生长量存在差异,表现出植株高矮和叶片大小的不同,但各指标的变化趋势基本一致。物候期比原产地提前30天。茎高生长起始时间早,速生期短,生长集中;叶生长的持续时间相对要长;叶宽生长的结束期更迟于叶长。3月12日—4月16日为这些紫斑牡丹茎生长高峰期,平均茎高生长量达23.56cm;4月24日已完成茎高全年生长量,年平均生长量为23.99cm。从3月下旬至4月中旬是叶片的迅速生长期,4月16日叶片大小定形,平均叶长、叶宽分别达到28.16cm和23.80cm。花期集中在4月16—26日,培育晚开新品种有重要意义。