简介：ThaijasminericeKDML105isconsumedaroundtheworld.BKOS,PKOSandTKOSarenewcultivarsproducedfromlow-energyionbeaminductioninKDML105.ThepurposeofthisstudyistocomparethemorphologyandanatomybetweenKDML105andthethreenewcultivars.Seedsofthefourcultivarsweregerminatedandgrowninpotsuntilfloweringphase.Theplants'organswereobservedandthelengthsofculms,ligules,leavesandpaniclesweremeasured.Leafsurfaceareawascalculatedandnumbersofroots,spikeletsandtillerswerecounted.BKOSandPKOShadsignificantlyshorterculmsthanKDML105andTKOS.ThelargestleafareawasfoundinKDML105followedbyTKOS,BKOSandPKOS,respectively.NumbersofrootsandtillersinBKOSandTKOSweresignificantlyfewerthanthoseinKDML105andPKOS.ThenumberofspikeletsperplantinBKOSwasthelowestamongallcultivars.Foranatomicalcomparison,crosssectionsofculmsandrootswereobserved.Allplantshadasimilararrangementoftissues,butthenumberandsizeofcellsweredifferent.Furthermore,longitudinalsectionsofculmsshowedthatthelengthsofepidermalandparenchymacellsweredirectlyrelatedwiththelengthoftheculm.Tocomparetheleaves,bothstomataandepidermalcellswerecountedandthelengthsoftheguardcellsweremeasured.ThelengthsofguardcellsofBKOSandPKOSwereshorter,butthestomataldensityandthestomatalindexweresignificantlygreaterthanthoseofKDML105.ForTKOS,thoughthelengthofguardcellswasshorterthanthatinKDML105,thedifferencewasnotsignificant.However,thestomataldensityandstomatalindexweresignificantlyhigherthanthoseinKDML105.

简介：Nearisogeniclinescarryinglarge-effectQTL(qtl12.1),whichhasaconsistentinfluenceongrainyieldunderuplanddroughtstressconditionsinawiderangeofenvironments,wereevaluatedunderwaterstressinthefields.Thelinewhichgavehigheryieldunderdroughtwascrossedwithalocaleliteline,PMK3,andforwardedtoF2:3generation.SignificantvariationwasfoundamongtheF2:3linesforagronomictraitsunderwaterstressinthefields.Lowtohighbroadsenseheritability(H)forinvestigatedtraitswasalsofound.Waterstressindicatorssuchasleafrollingandleafdryingwerenegativelycorrelatedwithplantheight,biomassandgrainyieldunderstress.Bulkedsegregantanalysis(BSA)wasperformedwiththemarkersinthevicinityofqtl12.1,andRM27933wasfoundtobesegregatedperfectlywellinindividualcomponentsofdroughtresistantanddroughtsusceptiblebulkswhichwerebulkedbasedonyieldunderwaterstressamongF2:3lines.Hence,thissimpleandbreederfriendlymarker,RM27933,maybeusefulasapotentiallyvaluablecandidatemarkerforthetransferoftheQTLqtl12.1intheregionalbreedingprogram.BioinformaticanalysisoftheDNAsequenceoftheqtl12.1regionwasalsodonetoidentifyandanalyzepositionalcandidategenesassociatedwiththisQTLandtoascertaintheputativemolecularbasisofqtl12.1.