简介:Mitogenactivated-proteinkinases(MAPKs)areimportantcomponentsinsignaltransductionpathwaysrespondingtovariousbioticandabioticstresses.AnMAPKgene,OsMPK14(GenBankAccessionNo.GQ265780)fromrice(OryzasativaL.),wasclonedbyRT-PCR.Thefull-lengthcDNAofOsMPK14consistsof1660bpinsize,containinganopenreadingframeof1629bp,whichencodesa542-amino-acidpolypeptideandhasatypicalproteinkinasedomainandaphosphorylationactivationmotifTDY.SequencealignmentandanalysisrevealedthatOsMPK14waslocatedonricechromosome5,andcomposedofnineexonsandeightintronsinthecodingregion.Semi-quantitativeRT-PCRwasperformedtodetecttheexpressionpatternsofOsMPK14inriceshootsandrootsunderdarkness,drought,highsalinity,lowtemperatureandabscisicacidtreatments.TheOsMPK14mRNAwasinducedbyabscisicacid,lowtemperatureandhighsalinity,butweaklyinhibitedbydrought.Inaddition,theexpressionofOsMPK14wasup-regulatedinroots,butdown-regulatedinshootsbylight.TheresultsindicatethatOsMPK14couldbeimplicatedindiversericestimuli-responsivesignalingcascades,anditsexpressionmightberegulatedbymultiplefactors.
简介:bispyribac钠10%SC和butachlor的效果土壤上的50%SC在移植秋收作物米饭的物理化学的性质和microflora在二个季节(2010和2011)被调查。体积密度,水成立能力,潮湿内容,土壤pH,有机物内容,电的传导性,以及全部的氮,可得到的磷和可得到的钾内容上的除草剂的效果与microflora人口(全部的细菌,放射菌类和真菌)一起被分析。在土壤的没有重要变化物理化学的性质被观察。除草剂处理开始在微生物引起的计数导致了减少。与在一可观的时间以内的应用除草剂的降级,microflora人口甚至在除草剂的申请以后在60d超过了起始的计数。