简介：目的观察分析巩膜扣带术治疗青少年陈旧性视网膜脱离的临床效果。方法回顾分析42例（42眼）陈旧性视网膜脱离青少年患者经巩膜扣带术进行治疗。术中直视下定位、冷凝视网膜裂孔。采用单纯巩膜外加压4眼（9.5%）,单纯巩膜外环扎6眼（14.3%）,环扎联合加压32眼（76.2%）。结果术后随访6-12个月（平均8.6个月）,36只眼（85.7%）一次手术后视网膜完全复位,6只眼（14.3%）明显好转。结论巩膜扣带术治疗青少年陈旧性视网膜脱离,疗效良好,创伤小,可作为首先选择的术式。

简介：目的：探讨阈值下微脉冲激光联合玻璃体注射抗血管内皮生长因子（vascularendothelialgrowthfactor,VEGF）治疗视网膜分支静脉阻塞（branchretinalveinocclusion,BRVO）继发黄斑水肿的疗效。方法：回顾性分析经荧光眼底血管造影（FFA）和相干光断层扫描（OCT）检查确诊为BRVO且病史〈3mo的BRVO继发黄斑水肿患者16例16眼,8眼予玻璃体腔注射抗VEGF药物治疗联合577nm阈值下微脉冲激光（微脉冲组）,8眼予玻璃体腔注射抗VEGF药物治疗联合传统格栅样激光光凝（格栅光凝组）。观察治疗前及治疗后6mo两组患者的最佳矫正视力（bestcorrectedvisualacuity,BCVA）及黄斑中心凹厚度（centralfovealthickness,CFT）。结果：治疗后6mo的BCVA：微脉冲组由治疗前的0.34±0.18提高到0.07±0.01,差异有统计学意义（P〈0.01）,格栅光凝组由治疗前的0.75±0.52提高到0.15±0.18,差异有统计学意义（P〈0.01）,两组治疗后比较差异无统计学意义（P〉0.05）。治疗后6mo的CFT：微脉冲组由治疗前的362.25±119.33μm降低至232.25±56.56μm,差异有统计学意义（P〈0.05）,格栅光凝组由治疗前的419.75±96.30μm降低至229.25±43.96μm,差异有统计学意义（P〈0.01）,两组治疗后比较差异无统计学意义（P〉0.05）。结论：治疗CRVO合并黄斑水肿,577nm阈下微脉冲激光联合玻璃体腔注射抗VEGF药物治疗与传统格栅光凝联合玻璃体腔注射抗VEGF药物治疗具有同样的良好疗效。

简介：目的探讨人工晶状体光学部后囊后植入技术,用于稳定眼内结构,预防后发性白内障的效果。方法对外伤性或与葡萄膜炎并发性白内障、某些先天性白内障术后或人工晶体移位后囊混浊等不易行激光后囊切开的患者,在完成皮质清除后,先把人工晶状体（IOL）袢固定于囊袋内或在机化囊膜夹持稳固下,将IOL光学部经后囊剪口植入后囊与玻璃体前界膜之间。结果全部术眼视轴透明,IOL光学部平稳居中,视功能恢复良好。结论对某些特殊型白内障术后后发障实施IOL光学部后囊后植入,可明显减轻眼内组织反应,预防后发障发生获得满意效果。

简介：目的：通过临床病例探讨和研究上睑退缩的分度,及选择不同的手术方案,并观察手术治疗效果,以便对不同患者提供更加有效的治疗方案。方法：回顾性总结2000-11/2010-12上睑退缩患者98例146眼,对其病情程度进行分度及根据其分度选择不同的手术治疗,观察其手术疗效。结果：针对不同分度选择适宜的手术方法,可以有效矫正眼睑退缩。结论：眼睑退缩由于病因及眼睑退缩的程度进行分度,对不同类型患者实施个性化手术,规范眼睑退缩临床治疗路径,填补国内同种疾病的诊断和治疗方法的空白。

简介：目的回顾性分析Nd：YAG激光后囊膜切开术治疗后发性白内障的疗效及安全。方法对52例（59眼）人工晶状体植入术后后发性白内障患者行Nd：YAG激光后囊膜切开术治疗。结果59眼一次手术成功,术后视力均有不同程度提高。结论YAG激光后囊膜切开术治疗后发性白内障简单、安全、有效。

简介：目的：观察4种上睑下垂术式的临床效果。方法：为探讨上睑下垂术的有效治疗方法，不断改进手术方式。结果：每种手术均有其优点及并发症，提上睑肌折叠术为目前较为理想的手术方法。结论：临床实践提上睑肌折叠术与以往专家理论：提上睑肌节制韧带上每缩短3～5mm肌肤，可矫正下垂量1mm不同：在节制韧带上缘折叠提上睑肌腱膜即可提起上睑，且上睑缘正好在角膜缘上2mm，术后恢复在角膜缘处。

简介：目的探讨复方樟柳碱对外伤性上睑下垂的疗效。方法将45例外伤性上睑下垂患者随机分为两组都给予改善微循环营养神经治疗,试验组加用复方樟柳碱,观察两组的疗效。结果试验组26例,16例达治愈标准,8例好转,2例无效。对照组19例,7例达治愈标准,4例好转,8例无效。两组比较,差异有显著意义（〈0.05）。结论复方樟柳碱对于外伤性上睑下垂效果明显。

简介：我科采用手术治疗外伤所引起的腱膜性上睑下垂患者48例，报告如下。

简介：AIM:Todeterminetheproliferativepotentialandthemaintenanceofstemcellactivityinstoredhumanlimbaltissues,andcorrelatethiswiththepreservationtime,cellviabilityandtheexpressionofstemcellmarkers.METHODS:Thirtylimbalrimsweresplitinto4partsandstoredincornealpreservationmediumat4℃for0,1,4,or7days.ThelimbalstemcellandmitoticmarkersP63,CK19,proliferatingcellnuclearantigen(PCNA),andKi67weredeterminedbyimmunohistochemicalstaining.Theproliferativepotentialoflimbalepithelialcellswasassessedbycellviability,theabilityofgeneratingstratifiedepithelium,andcolonyformingassay.RESULTS:Thestoredtissuesmaintainedlimbalstratifiedstructureto7daysandexhibitedcomparableexpressionlevelofstemcellandmitoticmarkers.Theproportionofviablecellsdecreasedwiththeprolongedpreservationtime,whilecolonyformingefficiencydecreasedfromthe1stdayanddisappearedatthe4thday.Wheninoculatedonamnioticmembrane,thecellspreservedfor1dayformedastratifiedepithelium,whilethecellsfrom4days’preservationformedadiscontinuouslayer.CONCLUSION:Thecolonyformingefficiencyoflimbalepithelialstem/progenitorcellsdecreasedrapidlywiththeincreasingpreservationtime,whiletheexpressionlevelofmarkersandcapacityofformingepithelialmonolayeronamnioticmembranedecreasedgradually.Thelimbalepithelialstemcellslosttheirfunctionearlierthanthelostexpressionlevelofstemcellmarkers.Thismayhelpustobetterchoosetheappropriatepreservationgraftsforfuturelimbalstemcelltransplantation.

简介：病例介绍病例1:患者,男,47岁。因右眼突然视力下降伴下方黑幕遮挡4天入院。原双眼有高度近视-14.00DS,入院时全身体检(-)。视力:右眼无光感,陈旧性视网膜脱离;左眼0.05(矫正)。左眼前节(-),眼底见9:30～4:00视网膜脱离,隆起度较高,累及黄斑区,颞上方约1:00时钟见一2PD大小马蹄孔。入院后第二天局部麻醉下行左眼冷凝加外加压加环扎术,外加压块、环扎带均位于赤道区,无放液并发症,手术顺利。术后第一天查眼底见脱离的

简介：患者男性,66岁.因进食梗咽1个月于2001年1月入院.胃镜检查示距门齿15～20cm处菜花样新生物,中央区缺损,质硬,占管腔1/2.GI示食管上段管壁增厚,最厚处2cm管腔狭窄,无胸水征.

简介：AIM:Topresentretinalmicrostructure,metabolismandfunctionabnormalitiesinthecourseofmultipleevanescentwhitedotsyndrome(MEWDS)byHeidelbergspectralismodalityimagingplatformandobserveitsoutcomebyEDI-SD-OCTandtwowavelengthautofluorescence.METHODS:Acaseofmultipleevanescentwhitedotsyndromeina23-year-oldfemalepresentedinitiallywitha15-dayhistoryoffloatersandacentralscotomaintherighteye.Toestablishthediagnosis,multimodalityimagingwasperformed,namely,bluelight-fundusautofluorescence(BL-FAF,excitation488nm,emission>500nm),near-infraredfundusautofluorescence(NIR-FAF,excitation787nm,emission>800nm)usingaconfocalscanninglaserophthalmoscope,fundusfluoresceinangiography(FFA),indocyaninegreenangiography(ICGA),spectrum-domainenhancedepthimagingopticalcoherencetomography(SD-EDI-OCT),multifocalelectroretinography(mf-ERG)andfundusphotograghwereperformedandfollowedupattheeighthmonthafterinitiallyvisiting.RESULTS:Opticalcoherencetomography(OCT)showedatransientdisruptionofthefovealphotoreceptoroutersegmentsincorrespondencetofovealgranularity.NIR-FAFshowedhypoautofluorescentareas,≤40μminsize,mostlyconcentratedaroundtheposteriorpoleanditstemporalsidelessthanthatinBL-FAF.Mf-ERGshowpinnacledisappearedinfoveaandmaculaandresponsesdecreasedmarkedlycomparedwiththefolloweye.Attheeighthmonthfollowup,hyperfluorescenceinBL-FAFweredisappear,while,NIR-FAFHypofluorescentspotsinearlystageofsuchlesionwerereduced.ButOCTdemonstratedthestructurewasrecoveredinresidualHypofluorescentareainNIR-FAF.Thesubfovealchoroidalthicknesswasdecreasedfrom372μmto307μmslightlyandcostlinewasrecovered.CONCLUSION:MEWDSisabenignself-healingdiseaseandthereisnopathologicalevidencetoinvestigatethenaturalcourseofsuchdisease.SD-OCTallowshighlydetailedimagesapproachinghistopathologytocertifythemicrostructura

简介：·AIM:Tostudytheeffectsofdanhonghuayukoufuye(DHK)onfastingbloodglucose(FBG)anddiabeticretinopathy(DR)instreptozotocin(STZ)-inducedtype1diabeticratstofacilitatetherationalusageofthisdrug.·METHODS:DiabeticratswereinducedbyinjectionofasingledoseofSTZintraperitoneallyat50mg/kg.Flashelectroretinogram(FERG)andoscillatorypotentials(OPs)wereusedtomeasureretinalfunction.Themicrovascularperfusionofearswasperformedtostudythemicrocirculationinrats.FBG,body-weight,and24-hurinevolume,waterintakeanddietintakewerealsoassessed.·RESULTS:DHKhadnoeffectonFBGinnormalrats.However,STZ+DHKgroupweresignificantlydifferentfromthoseofModelandmovedtowardthoseofnormalcontrol.Itreversedtheincreaseindietintake(P≤0.05vsmodelcontrol)andthelossinbody-weight(P≤0.05vsmodelcontrol)indiabeticrats.DHKdecreasedtheFBGofdiabeticratsby25.6%(P≤0.05)and37.9%(P≤0.01)after14and21daysadministrationascomparedwiththemodelcontrol,respectively.Moreover,DHKsignificantlyincreasedtheFERGb-waveamplitudeby80%(P≤0.05vsmodelcontrol)anddecreasedtheFERGb-wavelatencyby15.3%(P≤0.01vsmodelcontrol)after24daysadministration.TheOP1andOP2amplitudesinDHKgroupwere2.6(P≤0.01)and2.0(P≤0.01)timesofmodelgroupafter24daysofDHKtreatment,respectively.Atthesametime,OP1andOP2latenciesinDHKgroupreducedby16.0%(P≤0.001)and14.7%(P≤0.001)ascomparedwiththemodelcontrol,respectively.Furthermore,themicrovascularperfusionofDHKgroupwas2.4timesofmodelgroup(P≤0.001)after21daysadministration.·CONCLUSION:DHKhadnoeffectonnormalFBG.Butithadantihyperglycemicactivity,andhadapreventiveandtherapeuticeffectonDRindiabeticrats.·

简介：目的:探讨改良式额肌腱膜瓣悬吊术矫治儿童重度上睑下垂的长期临床疗效。方法：回顾性分析2009-01/2011-12在我科收治的儿童重度上睑下垂患者83例114眼，采用改良式扇形额肌腱膜瓣悬吊术进行矫治，分析术后上睑下垂矫正效果和上睑缘弧度、重睑形成及倒睫、结膜脱垂等并发症发生情况。随访观察5a。结果：术后第5a，矫正满意和基本矫正者共有84眼(73.7%)，部分矫正30眼（26.3%)，手术效果满意。术后并发症包括倒睫4眼（3.5%)，其中3例3眼上睑内侧1/3灰线处缝牵引缝线牵引lwk后消失，1例1眼行二次手术;结膜脱垂2眼（1.8%)，应用皮质类固醇激素眼药水点眼，加压包扎lwk后结膜脱垂均消失；术后无1例发生暴露性角膜炎、眼睑外翻、睑球分离、眼睑成角畸形、血肿形成、感染等并发症。术后上睑回退和外侧重睑皱襞消失是长期随访最常见的问题。结论：改良式额肌腱膜瓣悬吊术矫治儿童重度上睑下垂长期疗效满意，效果持久稳定，安全可靠。

简介：目的探讨采用提上睑肌折叠术治疗先天性上睑下垂的效果及可行性。方法对30例（40眼）中（3mm）重度下垂（≥4mm）的先天性上睑下垂行提上睑肌折叠术。结果本组30例（40眼）,随访2个月-1年,除2只眼发生轻度回退外,无未矫正病例,无穹窿部结膜脱垂,无暴露性角膜炎等并发症出现。结论提上睑肌折叠术治疗先天性上睑下垂是一种可靠有效的手术方法。

简介：AIM:ToinvestigatetheeffectofY-27632onthesurvivalandneuriteoutgrowthoftheculturedretinalneurocytes.METHODS:Afterthepostnatalday2-3,Sprague-Dawleyretinalneurocyteswereculturedfor48hours,theculturemediawasreplacedwithserum-freemedia(controlgroup)andserum-freemediacontained30μmol/LY-27632(Y-27632group),andthecellswerecontinuallyculturedanother48hours.Theculturedretinalneurocyteswereidentifiedwithanti-neuronspecificenolase(NSE)immunocytochemistry.ThesurvivalstateofthosecellswasestimatedbyMTTassay,andtheneuriteoutgrowthofthosecellswasevaluatedbythecomputerizedimage-analysissystem.RESULTS:Comparedwiththecontrolgroup,theabsorbancevaluesofcellssurvivalinY-27632groupincreased12.90%and33.33%respectivelyafter72and96hoursculture.Y-27632hadnosignificanteffectonthediameterofculturedretinalneurocytes.Comparedwiththecontrolgroup,Y-27632inducedastableimprovementofneuriteoutgrowthofretinalneurocytesafter72and96hoursculture(P=0.001).CONCLUSION:Y-27632couldpromotethesurvivalandneuriteoutgrowthoftheearlypostnatalculturedretinalneurocytes.

简介：AIM:ExcessivedissolveofcornealtissueinducedbyMMPswhichwereactivatedbycytokinsandchemokineswillleadtocornealulcer.ThemolecularmechanismofLipoxinA4(LXA4)oncornealcollagendegradationinthreedimensionswasinvestigated.·METHODS:Rabbitcornealfibroblastswereharvestedandsuspendedinserum-freeMEM.TypeIcollagen,DMEM,collagenreconstitutionbufferandcornealfibroblastsuspensionweremixedonice.Theresultantmixturesolidifiedinanincubator,afterwhichtestreagentsandplasminogenwasoverlaidandthecultureswerereturnedtotheincubator.Thesupernatantsfromcollagengelincubationswerecollectedandtheamountofhydroxyprolineinthehydrolysatewasmeasured.ImmunoblotanalysisofMMP-1,-3andTMMP-1,-2wasperformed.MMP-2,-9wasdetectedbythemethodofGelatinzymography.Cytotoxicityassaywasmeasured.RESULTS:LXA4inhibitedcornealcollagendegradationinadoseandtimemanner.LXA4inhibitedtheIL-1βinducedincreasesinthepro-MMP-1,-2,-3,-9andactiveMMP-1,-2,-3,-9inaconcentrationdependentmanner.LXA4alsoinhibitedtheIL-1βinducedincreasesinTIMP-1,-2.CONCLUSION:Asapotentanti-inflammationreagent,LXA4caninhibitcornealcollagendegradationinducedbyIL-1βincornealfibroblaststhusinhibitingcornealdissolvingpathologyprocess.

简介：1病理性近视1.1病理性近视继发脉络膜新生血管的流行病学研究和自然病程近视是最常见的屈光异常,高度近视在亚洲近视人群中所占比例为27%-33%,其中病理性近视（pathologicmyopia,PM）对视力造成严重影响[1]。据报道,成年人中PM的发病率为1%-3%,PM中继发脉络膜新生血管（choroidalneovascularization,CNV）的比率为4%-11%[2],其中位于中心凹的占58％，位于旁中心的占32％[3]。

简介：Thekeratoprosthesis(KPro;artificialcornea)isaspecialrefractivedevicetoreplacehumancorneabyusingheterogeneousformingmaterialsfortheimplantationintothedamagedeyesinordertoobtainacertainvision.Themainproblemsofartificialcorneaarethebiocompatibilityandstabilityofthetissueparticularlyinpenetratingkeratoplasty.Thecurrentstudiesoftissue-engineeredscaffoldmaterialsthroughcomprisingcompositesofnaturalandsyntheticbiopolymerstogetherhavedevelopedanewwaytoartificialcornea.Althoughawideagreementthatthelong-termstabilityofthesedeviceswouldbegreatlyimprovedbythepresenceofcorneacells,modificationofkeratoprosthesistosupportcorneacellsremainselusive.Mostofthestudiesoncornealsubstratematerialsandsurfacemodificationofcompositeshavetriedtoimprovethegrowthandbiocompatibilityofcorneacellswhichcannotonlyreducethestimulusofheterogeneousmaterials,butalsomoreimportantlycontinuousandstablecorneacellscanpreventthedestructionofcollagenase.Thenecrosisofstromaandspontaneousextrusionofthedevice,allowformaintenanceofaprecornealtearlayer,andplaytheroleofensuringagoodopticalsurfaceandresistingbacterialinfection.Asaresult,improvementincornealcellshasbeenthemainaimofseveralrecentinvestigations;someefforthasfocusedonbiomaterialforitswellbiologicalpropertiessuchaspromotingthegrowthofcorneacells.Thepurposeofthisreviewistosummarythegrowthstatusofthecornealcellsaftertheimplantationofseveralartificialcorneas.

简介：目的评价不增加切口的20G标准三通道玻璃体手术治疗严重眼外伤脉络膜上腔出血的疗效。方法对2011年10月至2014年10月间我科收治的29例（29眼）严重眼外伤合并脉络膜上腔出血的病人采取玻璃体手术治疗,置灌注后,通过板层的20G玻切穿刺口引流脉络膜上腔积血,再通过此切口行玻璃体手术,处理玻血和或网膜损伤,术终眼内填充气体或硅油。结果25例患者术中脉络膜上腔出血充分引流完全复位,4例患者残留少量积血,表现为小块脉络膜隆起,注入硅油后,术后一月复查网膜复位。所有患者术后视力均有改善。结论采用不增加切口的三通道玻切的巩膜切口可以有效引流外伤性脉络膜上腔出血,达到理想疗效。