简介：1SP细胞概述及分子机制在用流式细胞技术分析造血干细胞的过程中．有一小部分细胞表现为低的Hoeehst33342荧光染色特性．称为SidePopulation（SP）细胞。目前在鼠及人等哺乳动物的多种组织中通过相同的弱荧光染料染色现象已分离出相应的SP细胞。在造血干细胞研究中，低Hoeehst33342荧光染色SidePopulation现象最早在鼠的骨髓细胞制备过程中被Goodell等描述．

简介：Objective:tostudytheclinicalinfluenceoffemoralqualityoncementlesshipreplacementandtoevaluatecorticalindex(CI)forfemoralqualityinordertoguideprosthesischoiceandrehabilitation.Methods:forth-ninecasesofcementlesshipreplacementwerefollowedupforaverage3.5years,42ofwhonhadX-rayfilmsonpreoperationoroperationday.Results:Harrisscoresandpatients'satisfactionwerelowbutpainwassignificantandprolongedwhencorticalindiceswerelow.Conclusions:CIisareliablesemi-quantityparameterforclinicalevaluationoffemoralquality.Osteoporosispatientspredisposetothighpain.Lagforfull-weightloadingandavoidancefortorsionmotioncancontributetolesspainwhenCI≤2.2.

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简介：Objective:ToinvestigatetheeffectsofGinsenosideRb1ontheproliferationofSchwanncellsinculture.Methods:ApplyingMTTassayandThymidineincorporationassay,theeffectsofGinsenosideRb1ontheproliferationofSchwanncellsisolatedfromthesciaticnerveofadultratwerestudied.Results:GinsenosideRb1(10μg/ml)significantlyinducedSchwanncellproliferation,theeffectwassimilartoNGF(50μg/ml).AthighconcentrationsofGinsenosideRb1(1mg/ml),theproliferationofSchwanncellswassignificantlyinhibited.Conclusions:GinsenosideRb1attheoptimalconcentratiosisfoundtobeeffectiveininducingtheproliferationofSchwanncells,butathigherconcentrationsthedrugiscytotoxicforSchwanncells.

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简介：客观：在试验性的出血性的吃惊老鼠在心肌层和hepatocyte线粒体的功能上调查海水沉浸的效果。方法:24只男Wistar老鼠被划分成三个组(8在各个组织的n=)：控制组，HSL组(土地上的出血性的吃惊组)和HSS组(在海水的出血性的吃惊组)。血液动力学的参数，H~+-ATPase(adenosinetriphosphatase)的活动，succinatedehydrogenase(同步数字系列)和Ca~(2+)-Mg~(2+)-ATPase，在心肌层和hepatocytemitochondria的钙内容被测量，越过内部mitochondrialmembrane的质子translocation的变化被分析。结果：hernodynamic索引和H~+-ATPase的活动，同步数字系列，在HSS组的Ca~(2+)-Mg~(2+)-ATPase是比在控制组和HSLgroup的那些显著地低的(P<0.05)。在HSS组，在心肌层andhepatocyte的织物和线粒体的钙层次与控制组和HSL组相比显著地被提高(P<0.05)。在在三个组之中的质子translocation的Therewas不重要的差别。结论：Thisinvestigation证明那海水沉浸能加重出血性的吃惊老鼠的条件。

简介：Recombinantvacciniavirushasmanyadvantagesovermorerestrictedvectorslikeretrovirusandadenovirus.Theprovensafetyofvacciniavirus,whichisrestrictedtolocalandtransitoryinfection,favorsclinicalapplicationofvacciniavirustodelivercytokineslocally.

简介：客观：在山羊胫节在外皮的骨头灌注上把点contact-dynamiccompression板(PC-DCP)和它的效果的机械性质与动态压缩盘子(DCP)的作比较。方法：二十匹配的新鲜山羊胫节被使用。一个横向的破裂模型被建立。有在骨折结束之间的3mm间隙星际的破裂分别地与DCP和PC-DCPs服从于固定，然后，thefour指弯曲测试和扭转测试被进行把PC-DCP的机械性质与DCP的作比较。另一13只性成熟的山羊与DCPsand经历了固定PC-DCPs，分别地在未经触动的双边的胫节中间柄。Ischemic地区在四个次点被观察(1天，在操作以后的2,6，和12个星期)用disulphine蓝染色技术。结果：在机械性质没有重要差别，例如拐弯抵抗和扭转抵抗，在DCP和PC-DCPs之间。有天，在操作以后的2，和6个星期在DCP固定的方面上，在坚持的板下面的外部外皮的骨头局部缺血，和这个条件没在操作以后逆行直到12个星期。在PC-DCP固定的方面上，然而，外皮的骨头局部缺血仅仅在螺丝钉洞并且在PC坚果的接触地点的圆周发生了1天在操作，和它在操作以后消失在2个星期以后。结论：PC-DCP有类似的简历DCP的机械性质，但是比常规盘子对本地骨头血循环不太有害。

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简介：Objective:Tostudytheinfluenceofstress-relaxationplateondisorganizationandrepairofthecortexbeneaththeplate.Methods:Awashermadeofviscoelasticpolyethylenewasplacedbetweenthescrewandthescrewholeofconventionalstainlessrigidplate(RP)toproduceastressrelaxationplate(SRP).BothSRPandRPwereappliedtoosteotomizedtibiain48NewZealandrabbits.HealingprocessofthefracturewitheitherSRPorRPfixation(control)wascomparativelystudiedwithpolarizedlightmicroscopy,insituhybridizationofcollagenmRNAandimmunohistochemicaltechniquefrom2to36weekspostoperatively.Results:ThestudyofplatedboneremodelingshowedthatthedegreeofcortexosteoporosisbeneaththeplatewassimilarbetweentheSRPandRPgroupwithin12weekspostoperatively.Incomparison,thedisorganizationofbonestructureinSRPgrouphappenedlaterandmilderthanthatofRPgroup,andtherepairprocessbeganat12weeksafterimplantation.Asaconsequence,theabsorptioncavitiesbecamesmallerandthestructureofcollagenfibersbecamewellorientedalongwiththesechangesbypolarizedlightmicroscopy.Inadditiontothese,theinsituhybridizationanalysisofcollagengenesandtheimmunohistochemicalstudyoftypeI,Ⅲcollagenat8to12weeksafterimplantation.fromthistimeon,thechangesabovebecamemoreevidentsignificantlybeforemostofcavitieswererepairedby36weeks.IncontrasttothechangesintheSRPgroup,noexpressionandsynthesisofanykindofcollagencouldbeobservedduring12to36weeksafterimplantationinRPgroup.Conclusions:Withoutremovaloftheboneplate,theSRPfixationnotonlyreducesthedegreeofplatedboneosteoporosis,butalsomakesthedisorganizedbonestructurerestoredtonormalintermsoftheexpressionandsynthesisoftypeIcollagenmRNAofosteoblastslyingonthesurfaceofabsorptioncavities.

简介：Objective:ToexploretheexpressionofmRNAanditsproteininburnedratsandtheireffectsofburnwoundhealing.Methods:Apartial-thicknessburnof30%totalbodysurfaceareawascreatedonthebackof40Wistarrats.Insituhybridizationandimmunohistochemicalmethodswereusedtoexaluatethelocationandtheamountofthec-fosmRNAanditsproteininnormalskinandtheburnedskin,respectively,at3h,6h,1d,3d,7dand14dafterburn.Results:Underalightmicroscope,boththeexpressionofc-formRNAanditsproteincouldbefoundinthenormalskin,buttheirinductionlevelsweremuchhigherintheburnedskin.Thelevelofforproteinexpressionreachedpeakat3hafterburnwhilethatofc-formRNAreachedpeakat6hafterburn.Conclusions:Theexpressionofc-foscanbeinducedbyburns.Andthepeaklevelexpressionofc-formRNAcomeslaterthanthatofc-fosprotein.Itindicatesthattheactionoffosproteinisinducedbypost-translationalmodificationofpre-existingfosmolecules.

简介：客观：在兔子在针的绳索ischemia/reperfusion(I/R)以后在lipidperoxidation和apoptosis上学习白果树biloba摘录(GBE)的效果。方法：SpinalcordI/R损害模型根据对Erten等的描述被建立。27只NewZealand白兔子的一个总数随机被划分成三个组：一个假冒的组(9只兔子对待withs火腿操作但是没有大动脉的吸藏)，一个模型组(与大动脉的吸藏对待的9只兔子并且匹配卷盐)，并且一个GBE组(与大动脉的吸藏andGinaton(100mg/kg)对待的9只兔子在大动脉的夹钳前并且在灌注的发作注射了30分钟)。Theneurological结果分别地在灌注以后在24和48个小时被评估。针的绳索malondialdehyde(MDA)水平，超级氧化物dismutase(草皮)然后被检测。神经cellapoptosis被终端deoxynucleotidyltransferase(TdT)决定标记的-mediateddUTP-fluorescence刻痕结束(TUNEL)方法和bcl-2和bax的表示是examinedhistologically在有免疫组织化学的针的绳索。结果：I/Rproduced在神经病学的得分的重要减少。GBE组的马达分数比在在灌注以后的24和48个小时的模型组的那些显著地高(P<0.05)。与模型组相比，GBE改善了草皮的下面规定并且生产了MDA水平的重要减小(P<0.01)。为在模型组的TUNEL的积极房间是多于GBE组的那些的大部分(P<0.01)。bcl-2在I/R以后是起来调整的，特别在theGBE组(P<0.01)。bax的起来规定被GBE极大地减少(P<0.01).Conclusions：GBE对针的绳索I/R损害，和机制有保护的效果可以是它能清除氧释放激进分子并且禁止神经房间的apoptosis。

简介：Objective:Tostudytheeffectsofmagnesiumsulfateonbrainmitochondrialrespiratoryfunctioninratsafterexperimentaltraumaticbraininjuryandthepossiblemechanism.Methods:ThemiddledegreebraininjuryinratswasmadebyBIM-IIImulti-functionimpactingmachine.Thebrainmitochondrialrespiratoryfunctionwasmeasuredwithoxygenelectrodeandtheultra-structuralchangeswereobservedwithtransmissionelectronmicroscope(TEM).Results:1.ThebrainmitochondrialrespiratorystageIIIandrespirationcontrolratereducedsignificantlyintheuntreatedgroupswithin24and72hours.ButtreatedGroupAshowedcertaindegreeofrecoveryofrespiratoryfunction;treatedGroupBshowedfurtherimprovement.2.UntreatedGroup,treatedGroupsAandBhaddifferentdegreesofmitochondrialultra-structuraldamagerespectively,whichcouldbeattenuatedafterthetreatmentwithmagnesiumsulfate.Conclusions:Themitochondrialrespiratoryfunctiondecreasessignificantlyaftertraumaticbraininjury.ButitcanbeapparentlyimprovedaftermagnesiumsulfatemanagementalongwiththeattenuateddamageofmitochondriadiscoveredbyTEM.Thelongercourseoftreatmentcanobtainabetterimprovementofmitochondrialrespiratoryfunction.

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简介：探索骨头的效果的目的有带hepatocyte生长因素的adenoviral向量的导出髓的间充质的干细胞(BMSC)transfected(HGF，Ad-HGF)在灼伤创伤愈合上。从男Wistar老鼠的方法BMSC用由密度坡度centrifugation中等、与包含20%胎儿的牛的浆液(FBS)的DMEM有教养的Percoll分开被分开并且净化。当时，BMSC是有在感染(MOI)的100复合的最佳的基因transduction效率的Ad-HGF的transfected。transfection和在暂停的HGF的表示的效率被流动cytometry检测，酶分别地连接了immunosorbent试金(ELISA)。32只雌老鼠受到90慥?灳瑩吗？

简介：Objective:Toevaluatetheprotectiveeffectsof8%emulsifiedisofluraneaftermyocardialischemia-reperfusioninjuryanditsmechanisminrabbits.Methods:Twenty-fourmaleadultNewZealandwhiterabbitswereanesthetizedwithintravenousinjectionof30mg/kgpentobarbitalfollowedby5mg·kg-1·h-1infusion.Allrabbitsweresubjectedto30minutesofleftanteriorde-scendingcoronaryartery(LAD)occlusionand3hoursofsubsequentreperfusion.BeforeLADocclusion,therabbitswererandomlyallocatedintothreegroupsforprecondi-tioningtreatment(eightforeachgroup).Thecontrolgroup(Cgroup)receivedintravenously0.9%NaClfor30minutes.Theemulsifiedisofluranegroup(EIgroup)received8%emulsifiedisofluraneintravenouslytill0.64%end-tidalcon-centrationfor30minutesthatwasfollowedbya15-minutewashoutperiod.TheIntralipidgroup(INgroup)received30%Intralipidfor30minutes.Theinfarctedarea,plasmamalondialdehyde(MDA)content,superoxidedismutaseactivity(SOD)andnitriteconcentrationafter3-hourmyo-cardialperfusionwererecordedsimultaneously.Results:Forthemyocardialischemia-reperfusionin-juryanimals,theinfarctedsizeintheEIgroupwassignifi-cantlyreduced(91.9%±8%)ascomparedwithcontrolgroup(39%±6%,t=5.19,P<0.01).TheplasmaSODactivityandnitriteconcentrationinEIgroupweresignificantlyhigherthanthoseincontrolgroup(t=2.82,t=8.46,P<0.05),butMDAcontentwaslowerinEIgroupthanthatincontrolgroup(t=2.56,P<0.05).Conclusions:Theresultsindicatethatemulsifiedisofluranehasacardioprotectioneffectagainstischemia-reperfusioninjury.ThisbeneficialeffectofemulsifiedisofluraneisprobablythroughNOreleaseandconsequentlybyincreaseinautioxidationofmyocardium.

简介：探索二的保护的效果的目的与后部的手足的压碎损害在肠的mucosa障碍上ischemicpostconditioning(IP)录入兔子。这研究在损伤外科的部门在8月和2008年12月之间被进行的方法，弹跳的医院，第三所军事医药大学，重庆，中国。对兔子的后部的手足的压碎损害的模型被25kg目标第一开发，恰好后部的手足由木藤条修理了，然后IP的二种类型包括堵塞/打开被建立普通肠骨的动脉和静脉或者(传统的IP，IPA)并且binding/loosening受伤后部的手足的proximum或者(修改IP，IPB)。36只雄的新西兰白兔子随机被划分成三个组：IPA组，IPB组和控制组织，带着在每个组的12只兔子。肼oxidase(DAO)和肠的丰满的酸绑定的蛋白质(I-FABP)的浆液层次在损害以后在2，6，12和24个小时被检测。回肠的病理学的变化在损害以后在24个小时被检验。浆液在IPA和IPB组在损害以后在2，6，12和24个小时I-FABP铺平的结果有重要减少，与控制组相比。DAO层次也在损害以后在2和6个小时显示出一样的变化趋势，但是没显示出二个IP组之间的重要差别。在回肠的病理学的变化的差别都没在三个组之中被发现。结论IP能在兔子在后部的手足压碎损害的模型上保护肠的mucosa障碍功能。同时，修改IPB显示出象传统的IPA的一样的保护，并且值得在诊所适用。

简介：Objective:Toobservetheinfluenceofintra-articularinjectionofsodiumhyaluronate(HA)onthemRNAexpressionsofmatrixmetalloproteinase-1,-3(MMP-1,-3)andtissueinhibitorofmetalloproteinase-1(TIMP-1)incartilageandsynoviumoftraumaticosteoarthritis(OA).Methods:Sixteenwhiterabbitsunderwentunilateralanteriorcruciateligamenttransection(ACLT)weredividedinto2groupsrandomly5weeksaftertransection.Theexperimentalgrouprabbitsreceived0.3mlof1%HAbyintra-articularinjectiononceaweek.Animalsinthecontrolgroupweretreatedunderthesameconditionsusingphysiologicalsaline.Tenweeksfollowingsurgery,cartilageandsynoviumwereharvested.ThemRNAexpressionsofMMP-1,MMP-3andTIMP-1wereanalyzedusingreversetranscription-polymerasechainreaction(RT-PCR).Results:Insynovium,themRNAexpressionofMMP-3wassuppressedintheHAinjectiongroup.HAtreatmenthadnoeffectontheMMP-3expressionincartilage.NosignificantdifferenceofMMP-1andTIMP-1expressionsincartilageandsynoviumwasfoundbetweentheHAinjectiongroupandthecontrolgroup.Conclusions:OneofthemechanismsofthetherapeuticeffectofHAmaybetheinhibitionofexpressionofMMP-3insynoviumduringearlystageoftraumaticOA.

简介：Themoditicationoftumorcellsoreffcorcellsusingcytokinegenesasastrategytoenhancehostantitumorimmunityhasbeenstudiedintensivelyoverthe

简介：Objective:ToinvestigatetheeffectsofCO2pneumo-peritoneumonbloodflowvolumeofabdominalorgansofrabbitswithcontrolledhemorrhagicshockmodelandliverimpactinjuries.Methods:Aftercontrolledhemorrhagicshockandliverimpactinjuries,therabbitmodelwasestablished.Eighteenrabbitssubjectedtohemorrhagicshockandliverimpactinju-riesweredividedinto3groupsrandomlyaccordingtothevolumeoflostblood:lighthemorrhagicshock(bloodlossvolumewas10%,6ml/kg),moderatehemorrhagicshock(20%,12ml/kg)andseverehemorrhagicshock(40%,22ml/kg).IntraabdominalpressuresofCO2pneumoperitoneumwas10mmHg.Color-labeledmicrosphereswereusedtomea-surethebloodflowvolumeoftheliver,kidneyandstomachbeforepneumoperitoneumat30minutesand2hoursafterpneumoperitoneumand30minutesafterdeflation.Andthemortalityandhepatictraumaticconditionofrabbitswererecorded.Results:Ofthe18rabbits,therewere9withliverimpactinjuriesatGradeⅠ,8atGradeⅡandⅠatGradeⅢ(accordingtoAIS-2005).Themortalityrateinlighthemorrhagicshockgroupwas33.33%,andthatinmoderateorseverehemor-rhagicshockgroupwas100%within30minutesand2hoursafterpneumoperitoneum,respectively.Thebloodflowvol-umeintheorgansdetecteddecreasedat30minutesunderpneumoperitoneuminlightandmoderatehemorrhagicshockgroups.Atthesametime,thebloodflowvolumeoftheliverinmoderatehemorrhagicshockgroupdecreasedmoresig-nificantlythanthatinlighthemorrhagicshockgroup.Conclusions:ThebloodflowvolumeofabdominalorgansinrabbitsisdecreasedobviouslyunderCO2pneumoperitoneum,withfairlyhighmortalityrate.Itisbe-lievedthatCO2pneumoperitoneumshouldcautiouslybeusedinabdominalinjuryaccompaniedwithhemorrhagicshock,especiallyundernon-resuscitationconditions.