简介：探寻可替代甘油并简化复温后洗涤过程的较理想的红细胞低温保护剂，为低温保存红细胞临床应用提供便利。依据血红蛋白溶液浓度与其在波长为412nm处吸光度的关系曲线，建立红细胞回收率评估方法，研究比较了使用5种低温保护剂实现人类红细胞液氮深低温保存效果的优劣。结果表明：35.5％（w／v）甘油的保存效果最佳，其回收率为97．84％；其次为25％（w／v）右旋糖酐40，其回收率为85．08％；再次之为1M海藻糖（加载并孵育17．5h），其回收率为55．35％；回收率最低的两组为1M海藻糖（未经孵育）和10％（w／v）的二甲亚砜，对应的回收率均不足35％。初步推断：右旋糖酐40是一种可望替代甘油的红细胞低温保护剂。

简介：据vanderHarstP2012年12月6日（Nature,2012.doi：10.1038/nature11677.）报道,在一项新的研究中,研究人员揭示出红细胞是如何形成的和身体如何调节包裹在红细胞中的血红蛋白数量。研究人员还利用基因组分析技术让可能参与红细胞形成的基因区域数量增加了1倍,随后对果蝇进行研究。研究人员利用全基因组关联研究鉴定出似乎影响红细胞形成和它们的血红蛋白含量的基因区域。

简介：观察氩氦刀冻融的肺癌细胞联合IL-2对正常人外周血单个核细胞免疫功能的作用.分离正常人外周血单个核细胞,分别与氩氦冻融的肺癌细胞、IL-2和氩氦冻融的肺癌细胞联合IL-2混合培养,并设空白对照组.分别在第3、7、14天检测培养上清液中IL-12含量及NK细胞、CTL细胞的杀伤活性.结果表明,单用氩氦冻融的肺癌细胞可以轻度增加IL-12含量,但不能增加NK细胞、CTL细胞的杀伤活性;氩氦冻融的肺癌细胞联合IL-2后,可明显提高NK细胞和CTL的杀瘤活性.氩氦冻融的肺癌细胞联合IL-2对增强NK细胞和CTL活性具有协同作用,此种作用可能是通过刺激IL-12分泌增加实现的.

简介：测定实验组(60例CPB心脏手术)、对照组(20例非CPB心脏手术)围手术期红细胞C3b受体花环率、红细胞免疫复合物花环率;T淋巴细胞亚群;白细胞、红细胞、淋巴细胞计数.进行各时点指标与术前比较的方差分析(x±s),组间两两比较配对t检验;进行不同氧合器、CPB时间、体重、年龄等多因素比较的方差分析.探讨CPB对机体细胞免疫功能的影响及其主要影响因素.结果:实验组红细胞、T淋巴细胞免疫功能及淋巴细胞和红细胞计数均较术前显著下降,自停CPB持续至术后3～8天.白细胞计数在停CPB时下降,术后3～8天高于术前水平.CPB对细胞免疫功能的损害作用与CPB时间、手术失血量呈正相关;与病人年龄、体重、CPB温度呈负相关.不同性别、病种组免疫功能损害程度亦有一定差异.膜式氧合器组较鼓泡式氧合器组细胞免疫功能损害轻.结论:CPB的气血界面、血细胞破坏及人工管道系统均可导致血液有形成分破坏,损害机体的细胞免疫功能.在围手术期监护中应予以重视.

简介：研究氩氦刀治疗原发性肝癌后激活机体特异性T淋巴细胞的免疫效应。12例原发性肝细胞癌患者术前进行肿瘤组织穿刺活检确诊,活检组织制备肿瘤抗原;CT或B超引导下行氩氦刀消融,消融前、后放射免疫法测定血清AFP水平,以ELISPOT法测定特异性T细胞免疫活性。与治疗前比较,氩氦刀治疗后AFP水平短时间升高,随后下降;用肿瘤组织抗原可以诱导特异性T淋巴细胞活性(P＜0.01)。氩氦刀消融可有效激活肿瘤特异性的T细胞,瘤体来源的抗原可诱导特异性T淋巴细胞活性。

简介：研究氩氦刀治疗前列腺癌后，血清前列腺特异性膜抗原（PSMA）水平变化及特异性激活细胞毒性T淋巴细胞的效应。12例确诊前列腺癌患者，氩氦刀治疗前后EIA法检测PSMA，混合淋巴细胞反应检测特异性细胞毒性T淋巴细胞的增殖。结果表明：氩氦刀治疗后，血清PSMA升高，并可显著刺激特异性T淋巴细胞增殖，与对照组比较具有显著性差异（P〈0．01）。说明氩氦刀治疗可有效激活机体PSMA特异性的T细胞。

简介：Thequantumvibrationalenergy-spectraincludinghighexcitedstatesoftheproteinmoleculeshavebeencalculatedbynewtheoryofbio-energytransportalongtheproteinmoleculesanditsdynamicequation,discretenonlinearSchrodingerequation,appropriatetotheproteinmoleculesonthebasisofthelevelofmolecularstructure.Thisenergy-spectraobtainedarebasicallyconsistentwiththeexperimentalvaluesbyinfraredabsorptionandradiatedmeasurementofperson'shandsandlaser-RamanspectrumfrommetabolicallyactiveE.Coli..Fromthisenergy-spectraweknowthattheinfraredlightswith(1-3)x1000nmand(5-7)x1000nmwavelengthcanbeabsorbedbytheproteinmoleculesinthelivingsystems.Inaccordancewiththenon-lineartheoryofthebio-energytransportweknowthattheenergyoftheinfraredlightabsorbedbytheproteinscanresultinvibrationsofamide-Iinaminoacidsandcanfacilitatethebio-energytransportalongtheproteinmolecularchainsfromoneplacetootherforthegrowthoflivingbodies.Thisprocesseisnon-thermal.Thisisjustnon-thermaleffectoftheinfraredlights.Accordingtothemechanismweexplainedfurtherthemedicalfunctionsoftheinfraredlightsabsorbed.

简介：Osteocytesactasmechanosensorsinbone,whichcansendmechanicalsignalsdirectlytoosteoblaststhroughgapjunctions.However,underphysiologicalconditionsthenumberofgapjunctionsislimitedbecauseofthequantityvarianceofthetwokindsofcells.Inthisstudy,thepossibilityofindirectinteractionbetweenthesetwocellswasinvestigated.Anewflowchamperwasdesignedinwhichosteocytesandosteoblastswerecoculturedintwochampersseparately.OsteocyteswereexposedtofluidflowandthentheALPactivity,osteocalcinandosteopotinofosteoblastsweredetermined.TheresultsshowedthateitherALPactivityorproductionofosteocalcinandosteopotininosteoblaststhatwerecoculturedwithshearedosteocytesincreased,whichindicatedthatosteocytescouldregulateosteoblastsindirectlythroughsomesolublefactors.

简介：

简介：ImmobilizationusinglittlesplintisanoriginalinnovationofChinesepeopleforthefracturefixation,whichissimpletouseandclinicallyeffective.ItwasfoundthatChineseimmobilizationusinglittlesplintcanmakethenon-invasive,uncovering,andtroublefreehealingofbonefractureviaharmoniousunityofthestructurestabilityandtheforcebalance,ofthemotionstabilityandthestressadaptability,oftheconstantanddiscontinuousphysiologicalstress.ThebiomechanicaleffectofChineseimmobilizationusinglittlesplint,includingentirety,dynamic,andfunctionalfixity,istherootcauseofitsinheritanceandtheuseuptonow,andalsoisadirectionoftoday’sfracturefixationtowardspersonalization,individualityandentirety.

简介：ＥＦＦＥＣＴＯＦＨｅ－ＮｅＬＡＳＥＲＯＮＴＨＥＡＳＴＨＭＡＴＩＣＳＵＢＪＥＣＴＳＥＦＦＥＣＴＯＦＨｅ－ＮｅＬＡＳＥＲＯＮＴＨＥＡＳＴＨＭＡＴＩＣＳＵＢＪＥＣＴＳＧｕＧｕｏｘｉａｎ；ＷｕＳｕｙｉｎｇ；ＲｅｎＪｌｎｙｕｎ；ＷａｎｇＦｕｑｉａｎｇ（Ｄｅｐａ...

简介：ＣＬＩＮＩＣＡＬＡＮＤＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＹＯＦＫＩＬＬＩＮＧＥＦＦＥＣＴＯＮＨＥＬＩＣＯＢＡＣＴＥＲＰＹＬＯＲＩＣＬＩＮＩＣＡＬＡＮＤＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＹＯＦＫＩＬＬＩＮＧＥＦＦＥＣＴＯＮＨＥＬＩＣＯＢＡＣＴＥＲＰＹＬＯＲ...

简介：Theexperimentalstudywasperformedin78rabbits,theexposedanteriorven-tricularsurfaceoftheopen—chestrabbitswereimpactedwithapendulum—likemovementimpactorsystemwhichwascontrolledbytheCardiaccycle.Undrsomegivenconditions,theexperimentalresultswereasfollows:（1）Theimpactsat5.0m·s-1resultedinnocardiacruptureinallattemps（16/16）attheendofsystoleas

简介：ThetherapyofUBI(TheUltravioletirradiationoxygenateBloodItself-transfu-sion)isusedtotreat69casesofthepatientswithcerebralinfarctionwhichareallprovedtobecerebralinfarctionbythescanningofCT.TheeffectsofUBIonthenailfoldmicrocirculationofthepatientswerestudiedwith“WeightedMultipleScoreofthenailfoldmicrocirculation”workedoutbythemicrocirculationalspecialgroupP.L.A.Comparedwiththesameagedhealthyperson,theMultiplescoreofthenail

简介：Theeffectofage-relatedbonelossonthestructuralcapacityoftheproximalfemurwereinvestigatedbyFiniteElementAnalysis(FEA).Fourbonelosspatternswereconsidered.Thesewere'uniformcorticalboneloss','neckcorticalboneloss','intertrochantericcorticalboneloss'and'uniformtrabecularboneloss'.Theresultsshowthatthetwo'non-uniformcorticalboneloss'patternsaremoredangerousthanthe'uniformcorticalboneloss'pattern,andthatthecorticalbonelossinintertrochantericregionisassociatedwithagreaterreductionincorticalfailureloadthanthecorticalbonelossinthefemoralneck.Thetrabecularbonelosscausesalimiteddecreaseinbothcorticalfailureandtrabecularfailureloads.Thisresearchshouldbehelpfultotheclinicalassessmentoffemurfractureriskduetoage-relatedboneloss.

简介：Tocleanoutthesmearlayeronteethsurface,andprotecttheteethmedulla,theexperimentappliedthestudyobservingthecleanupofsixgroupsofsurfacesmearlayerofdentalcariesandthetubuleplugsreservingeffectafterusingofdifferentcavitycleanserswiththehelpofSEM.Theresultimpliedthatboththeacidicpreparationsandchelatorcouldcleanoutthesmearlayeron.teethsurfaceinsomeway,butthelateronecouldalsoreservepartialtubuleplugs,whichreducethestimulationtotheteethmedulla.Sothechelatorisbettercleansersinclinicaluse.

简介：SincetheinventionofRubylaserbyMaimanin1960s,manykindsoflasersareusedwidelyindentistry.Ithasbeenprovedthatlaserirradiationcanproduceacidetchinglikesurface,thusstrengthenthebondofmaterialtoenamel.Buttheheatgeneratedduringirradiationproducesdamageondentalpulp,whichlimitsthelaserapplicationsondentalhardsurfaces.

简介：Objective:Toobservetheeffectofstatinsonpreventingparoxysmalatrialfibrillation(PAF)afterpacemakerimlantationinpatientswithsicksinussyndrome.MaterialsandMethods:68patientswereselectedinwhichthepacemakershadbeenimplantedduetosicksinussyndrome,andwererandomlydividedintoastatintreatmentgroupandacontrolgroup.Afterthepacemakerimplantation,onlythepatientsintreatmentgroupweregiven20mgatorvastatinoncepernight,withotherconditionsbasicallysimilartothoseinthecontrolgroup.Atthe3rd,9th,15th,and21stmonthsaftertheimplantation,thepacemakerswereprogrammed,andthePAF-relatedinformationstoredinthepacemakerwererecalledandanalyzedstatistically.Results:Aftertheadministrationofstatinsfor9monthssincetheimlantation,theoccurrenceratesofPAFinthetreatmentgroupwasrelativelylowerthanthoseinthecontrolgroup.Afterfurtheradministrationofstatinsfor15months,boththeoccurrencerateofPAFandtheburdenofatrialfibrillationinthetreatmentgrouphadsignificantlydeclined.Aftercontinuousadministrationofstatinsfor21months,boththeoccurrencerateofPAFandtheburdenofatrialfibrillationinthetreatmentgroupweresignificantlylowerthanthoseinthecontrolgroup.Conclusion:Long-termadministrationofstatinscanreducetheriskofPAFaftertheimplantationofapacemakerinpatientswithsicksinussyndrome.

简介：Theresearchgroupwasinterestedinthetherapeuticpotentialoflimbalfibroblasts,whicharepre-sentinthecorneallimbus.Recently,inmuchstemcellresearch,researchersandadvocatesarestress-ingtheimportanceofutilizingcellsfromthenichewhichtheywilltarget.Thismeans,thatadiposetissueandbonemarrowderivedstemcells,arelikelynotappropriateandevendangeroustoapplyu-biquitouslyastherapiestootherpartsofthebodyincertaincases.Severalresearchgroupsandclini-caltrialshaveshownthistobethecase,includingcasesofblindness,braininflammation,anddeathasadverseevents.

简介：TheeffectofQiGongreflectedbyrheoencephalographyandheartratewerestudiedinthispaper.ThefunctionalrelationshipbetweenwaveamplitudeandtheheartratesreflectedbytwoprocessesofQiGong,“JingGong”forenergyprovoca-tive,and“FaGong”forenergyreleasehavebeencomparedandanalysed.28casesofwelltrained“QiGong”practitionersvolunteerinthisstudy.Therheoencepha-