简介：【 摘要】 目的 : 探讨白介素 － 6( IL- 6 ) ， 白介素 － 8( IL－ 8 ) 与胎膜早破 的关系 。 方法 : 收集 2018年 1月 -2019年 1月的 160例胎膜早破孕妇作为病例组 ; 随机抽取同期正常孕妇160例作为对照组 。 两组孕妇入院后即抽取静脉血提 取血清 ，采用 ELISA 法检测血清中IL－ 6 、IL－ 8的含量 ，统计分析病例组和对照组感染绒毛膜羊膜炎的几率 ，采用 western blot 测定胎盘组织中 IL－ 6 、IL－ 8含量 。结果 : 病例组血清中 IL－ 6 ,IL－ 8 的含量明显高于对照组 , 差异有统计学意义 ;病例组感染绒毛膜羊膜炎的几率明显高于对照组 ， 差异有统计学意义 ; 结论 :IL－ 6 ,IL－ 8 可能全程参与妊娠过程 ，因此联合监测 IL－ 6 、IL－ 8 的含量对于预测胎膜早破新生儿的预后有一定的作用 ， 尤其在降低新生儿死亡率 、预防远期并发症等方面有一定价值 。

简介：Thepurposeofthestudyistoestablishafluorescencequantitativereversetranscriptionpoly-merasechainresponse(FQ-RT-PCR)methodforthequantitativedeterminationofIL-2mRNAandIL-4mRNAinThcells,withwhichtheThcellsstatusofthepatientswithgynaecologicaltumorsandchronicrenalfailure(CRF)canbeanalyzed.IL-2cDNAandIL-4cDNAwereprepared,andtheplasmidpMD18carryingIL-2cDNAorIL-4cDNAfragmentwasconstructedandclonedasthetemplateforquantitativedetermination.Theprimersandprobeslabelledwith6-carboxy-fluorescein(FAM)and6-carboxy-tetrarnethylrhodamine(TAMRA)wereprepared,andtheexperimentalconditionswereoptimizedtosetuptheFQ-RT-PCRmethodforquantitativedeterminationofIL-2mRNAandEL-4mRNA.Thcellsenrichedfromperipheralbloodmononuclearcells(PBMCs)of20healthyvolunteers(HVs),16gynaecologicalbenign(GB)cases,18gynaecologicalmalignant(GM)tumorcasesand16chronicrenalfailure(CRF)patientsweretestedforIL-2mRNAandIL-4mRNAbyFQ-RT-PCR.Thehouse-keepinggeneβ-actinwasusedastheinternalcontrolgeneoftheexperiment.Thestandardcurveforlogconcentrationofseriesofquantitativetemplatesvsthresholdcycle(CT)wasestablishedbylinearregression,andthelinearrangewas102-107copies/μl.TheimprecisiontestshowedtheCVofinter-assayandintra-assayofahighcontentsamplebyFQ-RT-PCRwere7.8%and12.5%,respectively.TheCVofinter-assayandintra-assayofalowcontentsamplewere10.8%and19.5%,respectively.TheIL-2mRNAexpressionsinThofthepatientswithgynaecologicalmalignanttumor(comparedwiththeHVsandthepatientswithgynaecologicalbenigndisease)andinThoftheCRFpatients(comparedwiththeHVs)weredeclinedsignificantlyandatthesametimetheIL-4mRNAexpressionincreasedsignificantly(P<0.001).Asimple,sensitiveandaccurateFQ-RT-PCRmethodforthequantitativedetectionofIL-2mRNAandIL-4mRNAhasbeenestablished.TheIL-2mRNAandIL-4m

简介：TheaimofthisstudyistoinvestigatethefeasibilityandmechanismofhIL-2-preSDNAvaccineaspreventionandtherapeuticapproachagainstHepatitisB.EukaryonexpressionvectorinvolvinghIL-2andpreSgenewasconstructedwithrecombinanttechniqueandtransferredintonormalBALB/cmiceandHBVtransgenicmice(Tg-Mice)respectively.Tnenaseriesofdetectionwereperformed:detectionofanti-preS2,HBsantibodyandHBsAginBALB/cmiceandTg-micewithELISA,quantificationofHBVDNAcopiesinHBVTg-miceserumwithreal-timePCR,determinationofhepatitisdegreewithimmunopathologicalHEstaininganddetectionofliverfunction.Anti-preS1canbedetectedat4^th,6^thand10^thweekininoculatedBALB/cmice.Injectionwithgenegungainedanadvantageovermuscularandsubcutaneousinjectionsinceitacquiredjust1/10inoculationquantity(10μg/mouse).HighestexpressionofIgG2aat4^thweeksuggestedThl-mediatedimmuneresponse,whichfacilitatedHBVcleaning.OfallinoculatedHBVTg-mice,80%ofthemshowedanfi-preS2,HBsantibodypositiveandHBVDNAdecreased,and20%showednegativeforHBsAg.HEstainingtohepatictissueshowedobviousinfiltrationofinflammatorycells,swellingandgranulardegenerationofhepatocytes.Inourstudy,IL-2-preSDNAvaccinewhichcanprovokethehumoralandcellularimmuneresponseandbreaktheimmunetolerancesupportsthedesignationandconstructionofnewvaccineagainstHBVandspecificimmuneremedyforHBVcontinuousinfection.

简介：Triptolideisanatural,biologicallyactivecomponentderivedfromChineseherbTripterygiumWilfordiiHookF.(TWHF)whichiseffectiveintheclinicaltreatmentofautoimmunediseases,however,themechanismsbywhichtriptolideexertsimmunosuppressionremainfullyunderstood.Theprimaryofthisstudyistodemonstratewhethertriptolidecanaffectphenotype,cytokineproductionandallogeneicTcell-stimulatorycapacityofdendriticcells(DCs)whicharecriticalintheinductionofimmuneresponseortolerance.PhenotypicanalysisshowthattriptolidedoesnotaffecttheexpressionofMHC(Ia^b),CD80,CD86andCD40ofDCstimulatedwithornotLPS,butsignificantlyinhibitsIL12p70productionbyDCinadose-dependentmanner.Triptolide-treatedDCsexhibitareducedcapacitytostimulateproliferationofallogeneicCD4^+Tlymphocytes.Therefore,triptolide-mediatedimmunosuppressionmaydue,inpart,totheinhibitionofIL-12p70productionandimpairmentofallogeneicTcell-stimulatorycapacityofDCs.Ourresultsmayprovideapossiblemechanisticexplanationfortheeffectivenessoftriptolideinthetreatmentofautoimmunediseases.

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简介：TheexpressionofIL-4inaratmodelofchronicpulmonaryinfectionbiofilmformationinducedbyPseudomonasaeruginosawasinvestigated,inwhichSPFWisterratswereinfectedviatracheawith0.1mlP.aeruginosastrainPAO579(10~9CFU/ml)inalginatebeadsortheplanktonicformofthisbacterialstrain(10~9CFU/ml),andon3,7and14dafterinfection,thebacteriologicalandpathologicalchangeswereobservedaswellastheexpressionofthecytokineIL-4wasdetermined.ItwasdemonstratedthatthecountofCFUperlungtissueincaseofbacteriainalginatebeadswassignificantlyhigherthanthatofbacteriainplanktonicform,withmoreseveregrosspathologicchangesandinflammatoryreactionsinthealginatebeadgroupincomparisonwiththatoftheplanktonicforms(P=0.002,P=0.004andP=0.002,respectively).Inaddition,theexpressionofIL-4inthealginatebeadgroupwasalsohigherthanthatintheplanktonicform(P=0.02,P=0.02andP=0.022,respectively).Apositivecorre-lationbetweenthelevelofIL-4expressionandthegrosslungpathologyinalginatebeadgroupexistedasdemonstratedbysimpleregressionanalysis(r=0.78,P<0.02).Itisconcludedthatthechronicpul-monaryinfectionwithbiofilmformationinducedbyP.aeruginosatendstohavetheprioritytotheTh2immuneresponse.

简介：【摘要】目的：探讨对严重水肿患者使用超声引导置入 18G静脉留置针的价值。方法：纳入时间为 2017年 12月到 2018年 12月，纳入 32例来我院就诊的严重水肿患者，对所有患者均行超声引导置入 18G静脉留置针。分析穿刺成功率。结果： 32例严重水肿患者中， 1次穿刺成功的患者有 30例，其占比例为 93.75%（ 30/32）； 2次及 2次以上穿刺成功的患者有 2例，其占比例为 6.25%（ 2/32）；穿刺完成的平均时间为（ 6.31±0.21） min。输液中出现轻度液体渗漏的患者有 1例，其占比例为 3.13%（ 1/32）；将穿刺针拔出后，并采取针对性处理，病情明显好转。结论：对严重水肿患者使用超声引导置入 18G静脉留置针，可将严重水肿患者的穿刺成功率予以提升，将治疗效果提高。

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简介：摘要：目的：分析骨科手术患者应用不同麻醉方式及术中护理联合参芪颗粒对其血流动力学及血浆TNF-α和IL-6水平的影响。方法：选择2019年1月至2020年1月来我院接受骨科手术的患者68例，根据不同麻醉方式分成两组，分别为试验中34例，对照组34例，对照组患者采用硬膜外麻醉方式，试验组患者采用腰-硬膜外麻醉，同时给予术中护理及联合参芪颗粒，比较两组患者血流动力学及血浆TNF-α和IL-6的水平。结果：试验组患者在手术开始半小时和手术完成时DBP高于对照组患者，差异具有统计学意义（P0.05）。结论：对骨科手术患者采用不同麻醉方式及术中护理联合参芪颗粒，能有效改善患者DBP水平，促进骨折创面早日恢复，提高患者生活质量。