简介：Superoxidedismutase(草皮)是包括海洋的无脊椎动物在各种各样的有机体对反应的氧种类在抗氧化剂小径玩第一根防卫线的关键抗氧化剂酶。在那里主要存在二种特定的形式，Cu/Zn-SOD(SOD1)和Mn草皮(SOD2)，在优核质。草皮被知道被许多环境stressors并发地调制。由使用中央合成试验性的设计和反应表面方法，关节水完成温度(18-34栠杩?潨潭潬祧眠瑩?湫睯?湩敶瑲'O慲整洠汵楴爭灥慥?琭票潭楳獮

简介：ThisstudyexaminedtheeffectofsalinityontheexpressionofNa+/K+-ATPase(NKA)α-subunitandvacuolar-typeH+-ATPase(V-ATPase)β-subunitgeneinthegillofLitopenaeusvannamei.Semi-quantitativereversetranscription-polymerasechainreaction(RT-PCR)assayshowedthattheexpressionofNKAα-subunitandV-ATPaseβ-subunitgenewassignificantlyinfluencedbysalinity.ItwasfoundthattheNKAactivitysignificantlyvariedwithsalinityintimeanddosedependentmanner;whereastheV-ATPaseactivitydidnot.TheabundanceofNKAα-subunitgenetranscriptincreasedrapidlywhenthesalinitydecreasedfrom26bto21,andslowlywhenthesalinitydecreasedfrom26to31withinthefirst24h.Whenthesalinitydecreasedfrom26to21,thetranscriptionofNKAα-subunitgeneingillepitheliumwashigherat12hthanthatat0h,whichwasconsistentwiththeresultofimmunoblottingassayofNKAα-subunit.Inaddition,salinityhadasignificanttime-anddose-dependenteffectontheconcentrationofbiogenicaminesinbothhemolymphandgill.Ascomparedtootherparameters,theconcentrationofdopamine(DA)and5-hydroxytryptamine(5-HT)variedindifferentpatternswhenthesalinitydecreasedfrom26to21orincreasedfrom26to31,suggestingthatDAand5-HTplayeddifferentregulatoryrolesinosmoticadaptionandmodulationofshrimpwhensalinityvaries.