Nitrate reductase activity and its diurnal variation rhythm for Camptotheca acuminata seedlings

(整期优先)网络出版时间:2004-03-13
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Nitratereductaseactivity(NRA)indifferentplantorgansandleavesindifferentpositionsofCamptothecaacuminataseedlingswasdeterminedbyanInvivoassay,thediurnalvariationrhythmofNRAinleavesofdifferentpositionswasobserved,andthecorrelationsbetweenleafNRA,leafareaandlaminamassperunitarea(LMA)werealsoexamined.TheresultsshowedthatNRAintheleafwassignificantlyhighest,comparedwiththatinotherorganssuchasroots,stemsandleaves.Inthisexperiment,the10leaveswereselectedfromtheapextothebaseoftheseedlingsinorder.ThedifferentNRAoccurredobviouslyinleavesofdifferentpositionsofC.acuminataseedlingsfromtheapextothebase,andNRAwashigherinthe4th-6thleaves.ThediurnalchangerhythmofleafNRAshowedaonepeakcurve,andmaximumNRAvalueappearedataboutmidday(at12:30orso).NoobviouscorrelationsbetweenNRAandleafareaorlaminamassperunitareawereobserved.ThisstudyofferedscientificfoundationforthefurtherresearchonnitrogenmetabolismofC.acuminata.