Nestin+neuronshavebeenshowntoexpresscholineacetyltransferase(ChAT)inthemedialseptum-diagonalbandofBrocainadultrats.Thisstudyexploredtheprojectionofnestin+neuronstotheolfactorybulbandthetimecourseofnestin+neuronsinthemedialseptum-diagonalbandofBrocainadultratsduringinjuryrecoveryafterolfactorynervetransection.Thisstudyobservedthatallnestin+neuronsweredouble-labeledwithChATinthemedialseptum-diagonalbandofBroca.Approximately53.6%ofnestin+neuronswereprojectedtotheolfactorybulbandco-labeledwithfastblue.Alargenumberofnestin+neuronswerenotpresentineachregionofthemedialseptum-diagonalbandofBroca.Nestin+neuronsinthemedialseptumandverticallimbofthediagonalbandofBrocashowedobviouscompensatoryfunction.Thenumberofnestin+neuronsdecreasedtoaminimumlaterthannestin–/ChAT+neuronsinthemedialseptum-diagonalbandofBroca.Theresultssuggestthatnestin+cholinergicneuronsmayhaveacloserconnectiontoolfactorybulbneurons.Nestin+cholinergicneuronsmayhaveastrongertolerancetoinjurythanNestin–/ChAT+neurons.Thedifferencebetweennestin+andnestin–/ChAT+neuronsduringtherecoveryprocessrequiresfurtherinvestigations.
