摘要
ThewealthofDNAdatageneratedbythehumangenomeprojectcouplingwithrecentlyinventedhigh-throughputgeneexpressionprofilingtechniqueshasdramaticallyspeduptheprocessforbiomedicalresearchersonelucidatingtheroleofgenesinhumandiseases.Onepowerfulmethodtorevealinsightintogenefunctionsisthesystematicanalysisofgeneexpression.Twopopularhigh-throughputgeneexpressiontechnologies,microarrayandSerialAnalysisofGeneExpression(SAGE)arecapableofproducinglargeamountsofgeneexpressiondatawiththepotentialofprovidingnovelinsightsintofundamentaldiseaseprocesses,especiallycomplexsyndromessuchascardiovasculardisease,whoseetiologiesareduetomultiplegeneticfactorsandtheirinterplaywiththeenvironment.MicroarrayandSAGEhavealreadybeenusedtoexaminegeneexpressionpatternsofcell-culture,animalandhumantissuesmodelsofcardiovasculardiseases.Inthisreview,wewillfirstgiveabriefintroductionofmicroarrayandSAGEtechnologiesandpointouttheirlimitations.Wewillthendiscussthemajordiscoveriesandthenewbiologicalinsightsthathaveemergedfromtheirapplicationstocardiovasculardiseases.Finallywewilltouchuponpotentialchallengesandfuturedevelopmentsinthisarea.
出版日期
2002年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
