摘要
WeclonedcDNAsforXenopusaldolasesA,BandC.Thesethreealdolasegenesarelocalizedondifferentchromosomesasasinglecopygene.Intheadult,thealdolaseAgeneisexpressedextensivelyinmuscletissues,whereasthealdolaseBgeneisexpressedstronglyinkidney,liver,stomachandintestine,whilethealdolaseCgeneisexpressedinbrain,heartandovary.InoocytesaldolaseAandCmRNAs,butnotaldolaseBmRNA,areextensivelytranscribed.Thus,aldolaseAandCmRNAs,butnotBmRNA,occurabundantlyineggsasmaternalmRNAs,andstrongexpressionofaldolaseBmRNAisseenonlyafterthelateneurulastage.WeconcludethataldolaseAandCmRNAsaremajoraldolasemRNAsinearlystagesofXenopusembryogenesiswhichproceedsutilizingyolkastheonlyenergysource,aldolaseBmRNA,ontheotherhand,isexpressedonlylaterindevelopmentintissueswhicharerequiredfordietaryfructosemetabolism.WealsoisolatedtheXenopusaldolaseCgenomicgene(ca.12kb)andfoundthatitspromoter(ca.2kb)containsregionsnecessaryfortissue-specificexpressionandalsoaGCrichregionwhichisessentialforbasaltranscriptionalactivity.
出版日期
2002年02月12日(中国期刊网平台首次上网日期,不代表论文的发表时间)