摘要
Theeffectofnitrate(NO3-)onrice(OryzasativaL.)growthaswellasNabsorptionandassimilationduringdifferentgrowthstageswasexaminedusingthreetypicalricecultivars.Dryweight,yield,Nuptake,nitratereductaseactivity(NRA)inleaves,andglutaminesynthetaseactivity(GSA)inrootsandleavesduringtheirentiregrowthperiods,aswellasthekineticparametersofammonium(NH4+)uptakeattheseedlingstage,weremeasuredwithsolutioncultureexperiments.ResultsindicatedthatadditionofNH4+-NandNO3--Nataratioof75:25(NH4++NO3-treatment)whencomparedwiththatofNH4+-Nalone(NH4+treatment)increasedthedryweightof'Nanguang'cultivarby30%and'Yunjing38'cultivarby31%,andalsoincreasedtheirgrainyieldby21%and17%,respectively.Forthefourgrowthstages,thetotalNaccumulationinplantsincreasedbyanaverageof36%for'Nanguang'and31%for'Yunjing38',whereastheincreasingeffectofNO3-inthe'4007'cultivarwasonlyfoundattheseedlingstage.IntheNH4++NO3-treatmentcomparedtotheNH4+treatment,NRAintheleavesincreasedby2.09folds,andGSAincreasedby92%intherootsand52%intheleavesofthethreecultivars.NO3-supplyincreasedthemaximumuptakerate(Vmax)inthe'Nanguang'and'Yunjing38'cultivars,reflectingthattheNO3-itself,nottheincreasingNconcentration,increasedtheuptakerateofNH4+byrice.TherewasnoeffectontheapparentMichaelis-Mentenconstant(Km)ofthethreecultivars.Thus,somereplacementofNH4+withNO3-couldgreatlyimprovethegrowthofriceplants,mainlyonaccountoftheincreaseduptakeofNH4+promotedbyNO3-,andfuturestudiesshouldfocusonthemolecularmechanismoftheincreaseduptakeofNH4+byNO3-.
出版日期
2006年06月16日（中国期刊网平台首次上网日期，不代表论文的发表时间）
