Comparative studies on in vitro sperm decondensation and pronucleusformation in egg extracts between gynogenetic and bisexual fish-中国期刊网

摘要 Acell-freesystembasedupontheeggextractsfromgynogeneticgibelcarp(Carassiusauratusgibelio)orbisexualredcommoncarp(Cyprinuscarpioredvariety)wasdevelopedtoinvestigatedevelopmentalbehaviorsofthedemembranatedspermnuclei.Bothredcommoncarpandgibelcarpspermnucleicoulddecondensefullyandformpronucleiintheredcommoncarpeggextracts.Gibelcarpspermnucleicouldalsodecondensefullyandformpronucleiinthegibelcarpeggextracts,butredcommoncarpspermnucleicouldnotdecondensesufficientlyinthesameextracts.Thesignificantdifferencesofmorphologicalchangeswerefurtherconfirmedbyultrastructuralobservationoftransmissionelectronmicroscopy.Thedatafurtheroffercytologicalevidenceforgonochoristicreproductioninthegynogeneticallyreproducinggibelcarp.Inaddition,thespermnucleiinvitrodecondensationisdependentonthepHintheextracts,andthedecon-densedefficiencyisoptimalatpH7.However,noDNAreplicationwasobservedinthetwokindsofeggextractsduringtheincubationperiodofthespermnuclei.Itissuggestedthattheeggextractspreparedfromthegynogeneticgibelcarpshouldbeavalidinvitrosystemforstudyingmolecularmechanismongynogenesisandreproductionmodediversityinfish.

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Comparative studies on in vitro sperm decondensation and pronucleusformation in egg extracts between gynogenetic and bisexual fish

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Comparative studies on in vitro sperm decondensation and pronucleusformation in egg extracts between gynogenetic and bisexual fish

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