摘要
Araucariaangustifolia(Bertol.)O.Kuntzeexhibitsdimorphisminitsstemstructure,wherethetrunkisorthotropicandbranchesandbranchlets(primaryandsecondarybranches)areplagiotropic.Thesestemsexhibitdifferentbehaviorwhenusedforvegetativepropagation,andonlysegmentsoftrunkcanformacompleteplant.Thephysiologicalandbiochemicalmechanismsthatcharacterizethesestemsarestilllittleknown.Theaimofthisstudywastodescribethefreeaminoacidprofilesintrunks,branches,andbranchletsofA.angustifolia.Segmentsof5cminlengthwereexcisedfromyoungindividualsbelowthestemapex.Theneedleswereremovedandsampleswerefrozenandlyophilized.Thedeterminationsweremadebyhigh-performanceliquidchromatography,andtheresultswereexpressedaslg/gfreshweight(FW).Thetrunksandbrancheshadthehighestcontentoftotalaminoacids,whichwere112.23±20.57lg/gFWand111.97±27.78lg/gFW,respectively.Theaminoacids—glutamine,aspartateandc-aminobutyricacidandtyrosine—werenoticeablyhigherinthethreetypesofstems.Inthetrunk,ahigheramountofasparagineandtryptophan,wasalsodetected.Glutamicacidandglutaminewerefoundinhigherquantitiesinthebranches.Thebranchletshadverylowtotalaminoacidcontent(30.79±4.19lg/gFW),whereinasparagineistheonlyaminoacidnotdetected.Thus,itwasobservedthattheprofileofthefreeaminoaciddiffersamongtrunks,branches,andbranchletsinA.angustifolia,indicatingthattheyperformdifferentfunctions.
出版日期
2018年06月16日（中国期刊网平台首次上网日期，不代表论文的发表时间）
