简介:Objective:Toanalyzequantitativelythesynergisticandantagonisticeffectsofcombinedoxymatrine(OMT)and5-fluorouracil(5-GU)onacelllineofhumanlivercancer(HepG2)withmedian-effectprincipleinvitro.Methods:Themedian-effectprincipleandMTTmethodwereusedinthequantitativeanalysisofeffectsofthetwodrugs.Results:Cytotoxicactivityoftheindividualdrugsenhancedasdrugconcentrationincreased.Asfa=0.41,aCIequalto1indicatedadditivity;fa<0.41,aCIlessthan1indicatedsynergy;andfa>0.41,aCIgreaterthan1indicatedantagonism.Thesequenceofadministrationdidnotinfluencethecytotoxicactivityofthecombinedantitumordrugs.Theratioofdrugconcentrationwasafactorthatcaninfluencethekillingeffect.Conclusion:Thecombineddrugsinteraction(CI<1)wassynergisticatlowerconcentrationandantagonisticathigherconcentration.Theratioofdrugconcentrationisafactorthatcaninfluencethekillingeffect.
简介:TheDNAcontentandmorphometricfeaturesofhepatocellularcarcinoma(HCC)andlivercelldysplasia(LCD),includingnucleararea,nuclearperimeter,nuclearmaximumdiameterandnuclearcirclediameter,werequantitativelydeterminedbymeansofimageanalysistechnology.Theresultsshowedthatincomparisonwithnormalhepatocytes,LCDhadamarkedlyincreasedDNAcontentandnuclearmorphometricparameters,butthevalueswerelowerthanthoseforHCC.LCDshowedaslightincreaseinnuclearatypiarepresentedbythenuclearirregularindex,whichwasalsolessthanHCC.ThefindingsindicatethatLCDmaybeaprecaneerouslesionofHCC,tothecellsinanabnormalproliferativestate.
简介:Objective:ToexaminetheexpressionsofMDM2,P53andP27proteinsinchronicesophagitis,para-cancermucosaandesophagealcarcinoma.Methods:ImmunohistochemistrywasusedtodetecttheexpressionsofMDM2,P53andP27proteinsinforty-sevenpatientssufferingfromchronicesophagitisandeighty-fivecasesofesophagealcarcinomaandcorrespondingpara-cancermucosa.Flowcytometry((FCM)wasappliedtodetectthequantitiesoftheseproteinsexpressedinfreshtissuesof48casesofesophagealcancerandtheirpara-cancertissuesand24casesofrelativenormalmucosaatthesurfaceofcuttingedge.Results:Immunohistochemistryresultsshowedthattheexpressionsofthethreestudiedproteinswereverysimilarintheepitheliaofchronicesophagitisandpara-cancermucosa(P>0.05).BoththequalitativeandquantitativestudiesdisplayedthattheP53proteinhadnoexpressionanditsaccumulationswouldappearonlyintheearlystagesofesophaguscancerationwhiletheMDM2andP27proteinshaddifferentdegreesofexpressionsincasesofnormalesophagealmucosa.MDM2proteinmarkedlyincreasedintheadvancedstagesofesophagealcanceration.AquantitativestudyshowedthattheexpressionofP27proteinhadalinearityofdecreasingtendency(F=9.132,P=0.002)inthecourseofesophagealcanceration.Conclusion:Chronicesophagitismaybeaprecancerouslesion.OwingtothechangesoftheP53andP27proteins,wecanalsoconcludethattheseoccurintheearlystagesofesophagusoncogenesis,howeverthechangesofMDM2expressionmayoccurintheadvancedstageofesophagealcanceration.
简介:Objective:Hepatocellularcarcinoma(HCC)isaleadingcauseofcancer-relateddeaths.NovelserumbiomarkersarerequiredtoincreasethesensitivityandspecificityofserumscreeningforearlyHCCdiagnosis.ThisstudyemployedaquantitativeproteomicstrategytoanalyzethedifferentialexpressionofserumglycoproteinsbetweenHCCandnormalcontrolserumsamples.Methods:Lectinaffinitychromatography(LAC)wasusedtoenrichglycoproteinsfromtheserumsamples.Quantitativemassspectrometricanalysiscombinedwithstableisotopedimethyllabelingand2Dliquidchromatography(LC)separationswereperformedtoexaminethedifferentiallevelsofthedetectedproteinsbetweenHCCandcontrolserumsamples.Westernblotwasusedtoanalyzethedifferentialexpressionlevelsofthethreeserumproteins.Results:Atotalof2,280proteingroupswereidentifiedintheserumsamplesfromHCCpatientsbyusingthe2DLC-MS/MSmethod.Upto36proteinswereup-regulatedintheHCCserum,whereas19proteinsweredown-regulated.Threedifferentialglycoproteins,namely,fibrinogengammachain(FGG),FOS-likeantigen2(FOSL2),andα-1,6-mannosylglycoprotein6-β-N-acetylglucosaminyltransferaseB(MGAT5B)werevalidatedbyWesternblot.Allthesethreeproteinswereup-regulatedintheHCCserumsamples.Conclusion:AquantitativeglycoproteomicmethodwasestablishedandprovenusefultodeterminepotentialnovelbiomarkersforHCC.
简介:Objective:Thisstudyinvestigatedthecapabilityofdual-energyspectralcomputedtomography(CT)toquantitativelyevaluatelungperfusiondefectsthatareinducedbycentrallungcancer.Methods:Thirty-twopatientswithcentrallungcancerunderwentCTangiographyusingspectralimaging.Aunivariategenerallinearmodelwasconductedtoanalyzethevarianceofiodineconcentration/CTvaluewiththreefactorsoflungfields.Apairedt-testwasusedtocompareiodineconcentrationsandCTvaluesbetweenthedistalendoflungcancerandthecorrespondingareainthecontralateralnormallung.Results:Iodineconcentrationsincreasedprogressivelyinthefar,intermediateandneargroundsidesinthenormallungfieldsat0.60±0.28,0.93±0.27and1.25±0.38mg/mL,respectively(P<0.001).ThesametrendwasobservedfortheCTvalues[–(840.64±49.08),–(812.66±50.85)and–(760.83±89.17)HU,P<0.001].Theiodineconcentration(0.70±0.42mg/mL)ofthelungfieldinthedistalendoflungcancerwassignificantlylowerthanthecorrespondingareainthecontralateralnormallung(1.19±0.62mg/mL)(t=–7.23,P<0.001).However,theCTvalueoflungfieldinthedistalendoflungcancerwassignificantlyhigherthanthecorrespondingareainthecontralateralnormallung[–(765.29±93.34)HUvs.–(800.07±76.18)HU,t=3.564,P=0.001].Conclusions:SpectralCTimagingbasedonthespectraldifferentiationofiodineisfeasibleandcanquantitativelyevaluatepulmonaryperfusionandidentifyperfusiondefectsthatareinducedbycentrallungcancer.SpectralCTseemstobeapromisingtechniqueforthesimultaneousevaluationofbothmorphologicalandfunctionallunginformation.
简介:Objective:ToestablishafluoregenicprobequantitativeRT-PCR(FQ-RT-PCR)methodfordetectionoftheexpressionofMDR1geneintumorcellsandtoinvestigatetheexpressionofMDR1geneinpatientswithlungcancer.Methods:ThefluorogenicquantitativeRT-PCRmethodfordetectionoftheexpressionofMDR1genewasestablished.K562/ADMandK562celllinesor45tumortissuesfrompatientswithlungcancerwereexaminedonPEAppliedBiosystems7700SequenceDetectionmachine.Results:theaveragelevelsofMDR1geneexpressioninK562/ADMcellsandK562cellswere(6.86±0.65)×107copies/mgRNAand(8.49±0.67)×105copies/mgRNA,respectively.Theformerwas80.8timesgreaterthanthelatter.Eachsamplewasmeasured10timesandthecoefficientvariation(CV)was9.5%and7.9%,respectively.VariouslevelsofMDR1geneexpressionweredetectedin12of45patientswithlungcancer.Conclusion:QuantitativedetectionofMDR1geneexpressionintumorcellswasachievedbyusingFQ-RT-PCR.FQ-RT-PCRisanaccurate,andsensitivemethodandeasytoperform.Usingthismethod,lowlevelsofMDR1geneexpressioncouldbedetectedin24%ofthepatientswithlungcancer.