简介：AIM:ToanalyzetheassociationbetweenHelicobacterspp.andsomecommongutbacteriainpatientswithcholecystitis.METHODS:Anested-polymerasechainreaction(PCR),specificto16SrRNAofHelicobacterspp.wasperformedonparaffin-embeddedgallbladdersamplesof100cholecystitisand102controlcases.ThesampleswerealsoanalyzedforsomecommongutbacteriabyPCR.Positivesamplesweresequencedforspeciesidentification.RESULTS:HelicobacterDNAwasfoundinsevenoutof100casesofacuteandchroniccholecystitis.SequenceanalysisdisplayedHelicobacterpullorum(H.pullorum)insixcasesandHelicobacterpyloriinone;H.pullorumwasonlyfoundincaseswithmetaplasia.Controlsam-pleswerenegativeforHelicobacterspp.andsomecommongutbacteria.Therewasasignificantdifference(P=0.007)betweencholecystitisandcontrolsamplesforHelicobacterDNA.CONCLUSION:ApossiblerelationshipwasdetectedbetweenHelicobacterDNAandcholecystitis.Furtherserologicalandimmunohistochemicalstudiesareneededtosupportthesedata.

简介：瞄准：为了在Sprague-Dawley的远侧的食管分析microbiota移动，老鼠喂了一本高脂肪的食谱。方法：二十只Sprague-Dawley老鼠各被划分成高脂肪的饮食和10只老鼠的正常控制组。在从远侧的食管的mucosa的microbiota的作文基于选择文化被分析。许多乳杆菌种类被分子的生物技术识别。从乳杆菌殖民地的细菌的DNA被提取，并且16SrDNA被PCR用细菌的通用教材放大。放大16SrDNA产品被使中毒分开坡度胶化电气泳动(DGGE)。每个单身的乐队从胶化被净化并且送了被定序。结果：在远侧的食管把细菌的culturing基于mucosal，葡萄球菌aureus不在、全部厌氧微生物和乳杆菌种类与正常控制组相比在高脂肪的食谱组显著地被减少(P<0.01)。在远侧的食管的乳杆菌种类的作文上的详细DGGE分析揭示了那乳杆菌crispatus，乳杆菌gasseri(L。gasseri)并且乳杆菌reuteri(L。reuteri)在高脂肪的食谱组包括了乳杆菌种类，当在正常控制组的乳杆菌种类的作文由L组成了时。gasseri，乳杆菌jensenii和L。reuteri。结论：高脂肪的饮食在老鼠在远侧的食管导致了mucosalmicroflora移动，特别乳杆菌种类的作文。