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简介：D－riboseisaunique,5-carbonsugarthatoccursnaturallyinalllivingcells,D-ribosehasbeenusedasastaringmaterialforpreparationofsomecertainmedicinesorjustasanewnutraceuticalthathelpstheodynaturallyrestoreitsenergylevel,Thefermentativeprocessesarethebestfortheirrelativelysimpleseriesofproductionstepsandrelativelycheapstartingmaterials.However,becauseofthemanyimpuritiesinglucoseyeastwateranditsdarkcolor,pretreatmentandpurificationmustbedonetothefluidbeforeD-ribosecanbeseparated.Inthispaper,separationandpurificationofD-ribosewascarriedoutusingcationexchangeresins,ofwhichCa^2+exchangeresinhadthebesteffect.Theoptimalconditionforadsorptionisaflowvelocityof0.5BV/hatadsorptiontemperatureof25℃,Theoptimalconditionfordesorptionisaflowvelocityof0.5BV/hatdesorptiontemperatureof80℃。

简介：Baker'syeastmediatedreductionofopticallyactivediketoneisdescribed.Thetwoketogroupsareefficientlydifferentiatedandtheeevalueoftherecoveredmaterialisconsiderablyraised.Itaffordshighlyopticallyactivekeyintermediatesefficientlyforthesynthesisofnaturalpolyhydroxylatedagarofuranproducts.

简介：Thereareabout17chromosomesinyeastSaccharomycescerevisiae.Amiddlesizedchromosome,chromosomeV,waschoseninthisworkforstudyingandconstructingthephysi-calmaps.ChromosomeVfromstrainA364awasisolatedbypulsed-fieldgradientgelelectrophoresis(PFGE).GelslicescontainingchromosomeVDNAweredigestedwithtworarecuttingenzymes,NotⅠandSfiⅠ,andthree6-Ntrecognizingenzymes,SmaⅠ,SstⅡandApaⅠ.Severalstrategies-partialorcompletedigestions,digestionwithdifferentsetsoftwoenzymes,andhybrid-izationwithclonedgeneticallymappedprobes(CAN1,URA3,CEN5,PRO3,CHO1,SUP19,RAD51,RAD3)——wereusedtoaligntherestrictionfragments.Thereare9,9,15,17,and20sitesforNotⅠ,SfiⅠ,SmaⅠ,SstⅡandApaⅠrespectivelyinthemapoftheA364achromosomeV.Itstotallengthwascalculatedtobe620Kb(Kilo-bases).Thedistributionsofthecuttingsitesforthesefiveenzymesthroughthewholechromosomearenotuniform.Acomp-arisonbetweenthephysicalmapandthegeneticmapwasalsomade.

简介：Inordertostudythefunctionalstructureofthetranscriptionterminatorsandthemechanismoftemination,asurveyofthechromatinstructure,includingthelocationofDNaseIhypersensitivesitesandthenucleosomearrangement,ofyeastADH1andFLPterminatorswasmade.TheresultsshowthatthereisnorelationshipbetweenthefunctionoftheterminatorsandtheexistenceofDNaseIhypersensitivesites.However,itisfoundthatthereisalwaysanucleosmoeattheimmediateupstreamofthetranscriptionalterminationsites.Asacontrol,thechromatinstructuresofthepBR322DNAfragmentsontheyeastshuttervectorsarealsoinvestigatedatthesametime.TherandomnucleosomearrangementonthebacterialDNAinyesastagreeswiththepublishedreports.Anewhypothesis,aboutthemechanismoftranscriptionalterminationisputforwardandthereasonofdifferentnucleosomearrengementontheDNAswhichareoriginallyfromdifferentspeciesinyeastisdiscussed.

简介：Inordertostudythefunctionalstructureofthetranscriptionterminatorsandthemechanismoftermination,asurveyofthechromatinstructure,includingthelocationofDNaseIhypersensitivesitesandthenucleosomearrangement,ofyeastADH1andFLPterminatorswasmade.TheresultsshowthatthereisnorelationshipbetweenthefunctionoftheterminatorsandtheexistenceofDNaseIhypersensitivesites.However,itisfoundthatthereisalwaysanucleosomeattheimmediateupstreamofthetranscrip-tionalterminationsites.Asacontrol,thechromatinstructuresofthepBR322DNAfragmentsontheyeastshuttervectorsarealsoinvestigatedatthesametime.TherandomnucleosomearrangementonthebacterialDNAinyeastagreeswiththepublishedreports.Anewhypothesis,aboutthemechanismoftranscriptionalterminationisputforwardandthereasonofdifferentnucleosomearrangementontheDNAswhichareori-ginallyfromdifferentspeciesinyeastisdiscussed.

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简介：Thenewly-hatchednymphsofthesmallbrownplanthopper(SBPH),Laodelphaxstriatellus,includingfieldandsensitivepopulations,weresubjectedtothehigh-temperature(35°C)treatment.Thenumberofyeast-likeendosymbiotesinSBPHreducedby23.47%-34.23%,57.86%-61.51%and88.96%-90.71%afterthehigh-temperaturetreatmentfor1d,2d,and3d,respectively.However,thesizeofyeast-likeendosymbioteswasnotobviouslyaffected.ResistanceofSBPHtothreeinsecticides(imidacloprid,chlorpyrifosandfipronil)decreasedwiththeincreaseoftreatmenttime.

简介：提炼棕色的planthopper(BPH)，Nilaparvatalugens圣?l，是米饭的最破坏的害虫之一，被证实了在胖身体怀有似酵母的symbiotes(YLS)。几词法上不同的YLS以前被孤立并且在从BPH的vitro有教养，却直接证据正在缺乏进一步澄清有教养的YLS是否从BPH。在这研究，YLS的一种在vitro是成功地有教养的并且同时验证了由顺序分析在BPH的胖身体存在并且嵌套聚合酶链反应(PCR)。有教养的YLS在vitro孤立根据18SrDNA(ribosomalDNA)和5.8S-ITS(内部抄录分隔符)作为类Candida的一个成员被识别rDNA顺序和它从酵母的序列的种系发生的分析。因此，这酵母孤立作为象Candida一样symbiotes被说出。象Candida一样symbiotes被发现在胖身体，卵巢和BPH的最新生的卵，然而并非在头，存在胸并且中间勇气。另外，在1а的象Candida一样symbiotes的数字

简介：目的以分子生物学方法为“金标准”对两种商品化酵母样真菌鉴定产品RapidIDYeastPlus（简称RapIDYST）及API20CAUX（简称API20C）的鉴定效能进行评估.方法从2010年中国医院侵袭性真菌感染监测网菌株库中筛选酵母样真菌25种,共计194株.其中,包含临床最常见的5种酵母样真菌（白念珠菌、热带念珠菌、光滑念珠菌、近平滑念珠菌、新生隐球菌）共130株,占研究总菌株数的67.0％.所有菌株已经过分子生物学方法准确鉴定至种水平.菌株复苏分纯后,严格按照产品操作指南,平行进行RapIDYST和API20C鉴定.结果所研究菌株中,有181株（18种）在RapIDYST鉴定菌种数据库中,所有在库菌株种及复合体鉴定正确率为87.8％（159/181）.相比,API鉴定菌种库包含菌株174株（18种）,在库菌株种及复合体鉴定正确率为92.0％（160/174）.RapIDYST与API20C对于5种临床常见的酵母样真菌的种鉴定正确率分别为93.1％和97.1％.对于库外菌株,RapIDYST的鉴定错误率分别为23.1％（3/13）,相比API20C的鉴定错误率为60.0％（12/20）.综合此次评估结果,二者对酵母样真菌的鉴定效能无显著差异（McNemar检验,P＞0.05）.结论两种商品化产品对酵母样真菌的鉴定效能基本一致;相较而言,RapIDYST在操作便捷性、检测时间方面具有较大优势.