简介：RecentinsituhybridizationstudiesshowedthatmRNAlevelsofOLIGlandOLIG2transcriptionfactorsareelevatedinoligodendrogliomas.Weraisedpolyclonalantibodiesagainstasyntheticpeptidehomologoustothehumantran-scriptionfactorOliglandstudiedbyimmunohistochemistrytheexpressionofOliglin84braintumorsandinnon-neoplasticbraintissues.Alloligodendrogliomas,oligoastrocytomas,anddysembryoplasticneuroepithelialtumorsshowedmoderatetostrongintranuclearimmunoreactivityincellsmorphologicallyidentifiedasoligodendrocytes.In

简介：Toelucidatethemechanismofphotodynamicdamageofcells,theeffectofHPDpluslightontranscriptlonalectlvltyInthemucleusIsolatefromthenormalratliverwasstudiedinvitorby3H-UTPincorporationintoRNA.MeasurementsoffluorescencespectrumshowedthatHPDwasboundtothenucleusanditsfluorescenceIntensityIncreasedwiththeIncreaseofHPDconcentration.TheexperimentalresultsIndicatedthatnochangescouldbeobservedwheneitherHPDorlightwasusedalone.WhereasthenucleartranscriptionactivitywasfoundtobeinhibitedsignificantlybybothHPDandlighttreatment,andthedegreeofInhibitionwasdependentontheHPDconcentrationandthetimeofexposuretolight.Aftertreatmentby3μg/mlHPD,theinhibitionrateofthenucleartranscriptionactivitywas23%,45%,69%,80%and90%,respectivelyforlightexposureof2,5,10,20and30minutes.Ourresultssuggestedthatdose-dependentdecreasesinthenucleartranscriptionactivity,andmarkedinhibitionofthe