简介:Weisolatedandpurifiedmitochondriafrommouseliversandspinachleaves.WhenaddedintoeggextractsofXenopuslaevis,theycausednucleiofmouselivertoundergoapoptoticchanges.Chromatincondensation,marginationandDNAladderwereobserved.Afterincubatingisolatedmitochondriainsomehypotonicsolutions,andcentrifugingthesemixturesatmghspeed,wegotmitochondrialsupernatants.Itwasfoundthatintheabsenceofcytosolicfactor,thesupernatantalonewasabletoinduceapoptoticchangesinnuclei.Theeffectivecomponentswerepartlyofprotein.DNAfragmentationwaspartlyinhibitedbycaspaseinhibitorsAC-DEVD-CHOandAC-YVAD-CHO.Meanwhile,caspaseinhibitorsfullyblockedchromatincondensation.Primarycharacterizationofthenuclearendonuclease(s)inducedbymitochondrialsupernatantswasalsoconducted.ItwasfoundthatthisendonucleaseisdifferentfromendonucleaseG,cytochromec-inducednuclease,orCa^2+-activatedendonuclease.