简介：Despiterecentprogressinourunderstandingofcancerbiologyandinmanyareasofcancertreatment,thesuccessrateforcancertherapyremainsdismal.Immunotherapyforcancerhaslongbeenanexcitingfieldformanycancerresearchersduetothepossibilitytomobilizethebody'sownimmunesystemtoeradicatecancernotonlylocallybutalsosystemically.Sinceitsinitialdiscovery,cytokine-basedimmunotherapyhasbeenvigorouslyandextensivelyinvestigatedforcancertreatmentduetotheperceptionofitasarelativelyeasilypurifiable,injectableformofcancertreatmentagent.However,sofarmostcytokine-basedtherapytrialshavefallenshortofexpectations.Oneofmainobstaclesisthedifficultytoachievetherapeuticallyrelevantdosageinpatientswithoutgeneratingexcessivenormaltissuetoxicity.Theemergenceofnovelgenetherapyapproachtodelivertherapeuticcytokinetotumorslocallygeneratedgreatexcitementsinceithasthepotentialofgeneratingsustainedhighlocalconcentrationofimmunostimulatorycytokinewithoutraisingthesystemiclevelsofthecytokines,whichisresponsibleformostoftheobservedtoxicity.Inthisreview,wewillattempttoprovideanoverviewofthefieldanddiscusssomeoftheproblemsassociatedwithcytokine-basedimmuno-genetherapyandpotentialsolutions.Cellular&MolecularImmunology.2005;2(2):81-91.

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简介：牙槽的巨噬细胞(AMs)由释放许多cytokines和煽动性的调停人在肺的纤维变性的致病起一个关键作用，这被表明了。另外，反常信号变换器和在AMs的激活可以玩的transcription-1(STAT1)的使活跃之物在齿槽炎和肺的纤维变性的进程的一个枢轴的角色。在这研究，我们transfectedSTAT1antisenseoligodeoxynucleotide(ASON)进由aerosolization的老鼠，然后与导致的bleomycin(BLM)从老鼠在bronchoalveolarlavage液体(BALF)在象TGF-，PDGF和TNF-那样的煽动性的调停人上调查了STAT1ASON的效果老鼠肺的纤维变性。我们的结果证明由aerosolization的STAT1ASON能进入肺纸巾和AMs。STAT1ASON能与肺的纤维变性在老鼠的AMs禁止mRNA和STAT1和ICAM-1的蛋白质表情，并且没在肝和肾上有有毒的副作用。AerosolizedSTAT1ASON能改善通过在导致BLM的老鼠禁止煽动性的调停人的分泌物的齿槽炎肺的纤维变性。这些结果建议aerosolizedSTAT1ASON可能在肺的纤维变性的处理被看作有希望的新策略。