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  • 简介:Wedescribeanoveltechnique,lowsurfaceenergyGasExpansionMolding(GEM),tofabricatemicrobubblearraysinpolydimethylsiloxane(PDMS)whichareincorporatedintoparallelplateflowchambersandtestedincellsortingandmicrocellcultureapplications.Thisarchitectureconfersseveraloperationaladvantagesthatdistinguishthistechnologyapproachfromcurrentlyusedmethods.HereinwedescribetheGEMprocessandtheparametersthatareusedtocontrolmicrobubbleformationandaVacuum-AssistedCoating(VAC)processdevelopedtoselectivelyandspatiallyalterthePDMSsurfacechemistryinthewellsandonthemicrochannelsurface.WedescriberesultsfrommicroflowimagevisualizationstudiesconductedtoinvestigatefluidstreamsaboveandwithinmicrobubblewellsandconcludewithadiscussionofcellculturestudiesinPDMS.

  • 标签: 气体扩展模型 细胞培养物 细胞挑选 PDMS