简介：MarinesedimentsampleswerecollectedfromthecoastalareasofSouthernIndia,particularlyinKanyakumariDistrict.Twenty-eightdifferentfungalstrainswereisolated.Thescreeningoffungifrommarinesedimentwasdonetoisolateapotentfungusthatcanproducebioactivecompoundsforbiomedicalapplications.OnlythreestrainsvizTrichodermagamsiiSP4,TalaromycesflavusSP5andAspergillusoryzaeSP6werescreenedforfurtherstudies.Theintracellularbioactivecompoundswereextractedusingsolventextractionmethod.Thecrudeextractsweretestedforitsanti-microbialandanti-cancerpropertiesandanalyticallycharacterizedusingGasChromatographyMassSpectrometry(GC-MS).Allthethreeextractswereactive,buttheextractfromT.flavusSP5wasfoundtobemoreactiveagainstvarioushumanpathogens,viz.,PseudomonasaeruginosaATCC27853(17.8±0.1),EscherichiacoliATCC52922(18.3±0.3),andCandidatropicalisATCC750(17.7±0.4).ItalsoexhibitedcytotoxicactivityagainstHEp2carcinomacelllinewiththeLC_(50)valueof25.7μg·mL~(-1).TheGC-MSdatarevealedthepresenceofeffectivebioactivecompounds.TheseresultsrevealedthattheextractfromisolatedfungusT.flavusSP5actedasapotentantimicrobial,antifungal,andanticanceragent,providingbasicinformationonthepotencyofmarinefungitowardsbiomedicalapplications;furtherinvestigationmayleadtothedevelopmentofnovelanticancerdrugs.