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1 个结果
  • 简介:Objective:ToanalyzetypeⅡtopoisomerasegenesinclinicalisolatesoffluoroquinolone-resistantMycoplasmahominis.Methods:EightisolatesofM.hominiscrossresistantto6fluoroquinoloneswereselectedfrom103clinicalstrainsofM.hominisusingabrothmicrodilutionmethod.TypeIItopoisomerasegeneswereamplifiedbyPCRanddirectlysequenced.Nucleotidesequenceswerecomparedtosequencesfromasusceptiblestrain(M.hominisPG2I).Results:MICsofresistantMhisolateswere4-to512-foldhigherthanMICsfromthesusceptiblereferencestrain.SequencecomparisonrevealedaCtoTchangeat113ntingyrAQRDRledtothesubstitutionofSer83byLeucineandnoaminoacidchangeingyrB.AchangeofGtoTat134ntinparCQRDRledtothesubstitutionofSer80byIsoleucineandaGtoAchangeat70ntinparEQRDRledtothesubstitutionofAsparticacidbyAsparagine.Conclusion:TheseresultssuggestthataCtoTchangeatll3ntingyrA,aGtoTchangeat134ntinparCandaGtoAchangeat70ntinpatrEareassociatedwithfluoroquinoloneresistanceofM.hominis.

  • 标签: 支原体感染 基因突变 临床研究 PCR 聚合酶链反应 核酸