简介:NuclearfactorkappaB(NF-κB)isoneofthebest-characterizedtranscriptionfactorsplayingimportantrolesinmanycellularresponsestoalargevarietyofstimuli,includinginflammatorycytokines,phorbolesters,growthfactors,andbacterialandviralproducts.TheaimofthisstudyistodemonstrateNF-κBexpressioninthemousecochleaanditsenhancementinresponsetolipopolysaccharides(LPS)andkanamycin(KA)treatment.MethodsKAtreatmentconsistedofsubcutaneousKAinjectionsat700mg/kgtwiceadaywithaneight-hourintervalbetweenthetwoinjectionsfor3or7days.ForanimalsintheLPStreatmentgroup,asingledoseof0.3mgLPSdissolvedin0.2mlsterilesalinewereinjectedintobothbullaethroughthetympanicmembraneandkepttherefor3hours.Animalsinthecontrolgroupreceivedsubcutaneoussalineinjectionfor7days.Followingimmmunohistochemichalprocessingwithrabbitpolyclonalanti-NF-κBp65antibodies,cryosectionsofthecochleawereexaminedforexpressionofNF-κBp65invariousstructuresinthecochlea.ResultsNF-κBp65expression,identifiedbypresenceofbrownreactionproductscharacteristicofDABimmunohistochemistry,wasvisibleinthespiralligament,spiralprominence,tectorialmembrane(TM),spiralganglionandnervefibers.RelativelyweakNF-κBp65expressionwasalsovisualizedintheorganofCorti.WithintheorganofCorti,theinnerhaircells(IHC),outerhaircells(OHC),innerpillarcells(IP),outerpillarcells(OP),Deiter'scells(DC),andBoettcher'scellsexhibitedstrongerstainingthantheinnersulcuscells,Hensen'scells(HC)andClaudius'cells.NoNF-κBp65expressionwasseeninthenucleusoftheIHCandOHC.NF-κBp65expressionwasincreasedinanimalsexposedtoLPSorKA,demonstratingsignificantdifferencesinthestainingbetweencontrolanimalsandLPS/KA-treatedanimals.NF-κBp65expressionwasnotsignificantlydifferentbetweenLPStreatedandKAtreatedanimalsorbetween3and7daysinKA-treatedanimals.Conclusio
简介:摘要目的:研究近视患者双眼暗视至明视状态下Kappa角水平偏移量及垂直偏移量的变化规律。方法:横断面研究。选取2020年11月至2021年12月在宁夏医科大学总医院门诊近视中心明确诊断为近视的患者,并采用单纯随机抽样的方法抽取120例(240眼),采用博士伦Orbscan II角膜地形图仪分别于暗视及明视条件下进行检查,记录明暗视下瞳孔直径大小、Kappa角的大小及水平偏移量和垂直偏移量。采用配对t检验比较左右眼在暗视和明视下的瞳孔直径和Kappa角大小;采用散点分布图分析双眼在明暗视下Kappa角的象限分布。结果:左右眼在暗视状态下的瞳孔直径均明显大于明视状态下的瞳孔直径,差异有统计学意义(t=13.67,P<0.001;t=13.48,P<0.001)。暗视与明视状态下左眼的Kappa角均大于右眼的Kappa角,差异有统计学意义(t=4.15,P=0.021;t=5.27,P=0.008)。由暗视转向明视状态时,双眼Kappa角的分布呈现镜像对称,水平方向主要向鼻侧位移,垂直方向主要向上方位移。右眼矢量位移(0.168±0.100)mm,左眼矢量位移(0.171±0.069)mm;87.5%右眼矢量位移小于0.3 mm,92.5%左眼矢量位移小于0.3 mm,绝大多数近视患者的矢量位移在0.3 mm以内。结论:从暗视至明视状态下,随着瞳孔缩小,近视患者的Kappa角分布主要向鼻上方位移。
简介:对于两个相依线性回归方程组成的系统(1.1),本文提出了β1的待定系数估计β^*1(k,c)=(x′1x1+k1)^-1(x′1y1-cσ12/σ22x′1N2y2),其中岭参数k≥0.c是待定系数.与β^*1(k,c)对应的非限定两步估计记为β^41(T,k,c).当c=1时β^*1(k,1)=β1(k)和β^*1(T,k,1)=β1(T,k)等干[6]引入的一双有偏估计,结果表明总可以选取适当的c值和k值使β^*1(k,c)和β^*1(T,k,c)在均方误差阵准则下分别优于β1和β1(T),并讨论了c值的最佳选择问题.
简介:通过引入有效应力集中系数修正Lemaitgre-plumtree疲劳模型,证明了在含微缺陷结构中缺口处的有效应力集中系数与疲劳极限存在的相关关系,即K1=α-1/α'-[1];建立了以有效应力集中系数为自变量的损伤变量D(K1)的表达式,阐述了其损伤力学性质;最后推导出有效应力集中系数与腐蚀影响系数间的关系表达式。