学科分类
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4 个结果
  • 简介:ThenewspreadingconchosporesotPorphyrayezoensis,P.haitanensisandP.katadaivar.hemiphyllaareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthat(1)nomutantshavebeeninvestigatedinallcontrolgroup.(2)TheconchosporesofallthethreespeciesofPorphyraareeasytobeinducedbyNGfortheyareinthestageofbreeding,Inthelowestdose(10μg/mL)ofthisexperiment,themutantrateofP.yezoensisisupto57.9%,thatofP.haitanensis30.7%andthatofP.katadaivar.hemiphylla51.7%.(3)Themutantratecannotincreaseobviouslybyincreasingthereagentconcentrationorprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofthreespeciesofPorphyraaremuchsimilar,andthemutantrateisnear50%.TheoptimuminductionconcentrationofNGis10μg/mL,andtheoptimuminductiontimeis30minutes.

  • 标签: Porphyra MUTANT of chromatophore conchospore N-Methy-N'-Nitro-NNitrosoguanidine
  • 简介:AneuploidyembryosofPinctadamartensiiDunkerareproducedduringtetraploidinductionbyinhibitingthefirstpolarbodyineggsfromtriploidfertilizedwithhaploidspermswithcytochalasinBtreatment.Chromosomeanalysisrevealsthatthereare88.18±6.79%aneuploidyembryos,and28.70%aneuploidsinpearloystersofone-yearageTheseaneuploidshavefivechromosomalconditions,suchas2n+1(29),2n+2(30),3n-2(40),3n-1(41)and3n+1(43).Resultsofgrowthmeasurementshowthatthereisnosignificantdifferencebetweenaneuploids(asagroup)anddiploidsinbodysizeandweight(p>0.10),buttheaneuploideisobviouslydifferentfromtriploid(p<0.01).Themeanbodysizeandweightofaneuploidsindiploidcondition(2n±1and2n±2)aresignificantlysmallerthanthoseofdiploids(p<0.01),butaneuploidswithintriploidcondition(3n±1and3n±2)arenotsmallerthandiploidsinbodysizeandweight(p>0.1).ThisstudyindicatesPinctadamartensiiDunkercouldtolerateaneuploidyby7~14%ofthehaploidgenome,andthataneuploidsofthisspeciesareviableundercertainconditions.

  • 标签: aneuploid triploid PINCTADA martensii Dunker
  • 简介:TheyoungthalliofPorphyrayezoensisandP.haitanensisareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthatnomutantshavebeeninvestigatedinallcontrolgroups,whileintheinducedgroups,itiseasytofindmutantcellsormutantcellmassesinthethallus.ThepigmentationmutantsmaybetheresultofNGinducement.Themutantrateincreasesobviouslywiththemutagenconcentrationorbyprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofPorphyrayezoensisareraisedfrom11.2%to28.7%,andthoseofP.haitanensisfrom10.1%to20.2%.Accordingtoourexperiments,theamountofmutantcellsinthesameareadoesnotincreasewiththeinductionintensityTheamountofmutantcellsineverygroupofinducementofbothspeciesofPorphyrachangesverylittlewiththefixedfieldscheckmethod.Thelowestdose(25μg/mL)ofthisexperimenthasahighmutantrate.Themutantrateshowsthepercentageofmutantcellsinallthesurvivedcells,whiletheamountofmutantcellsreflectstheeffectofinducement.ThustheoptimuminductionconcentrationofNGis25μg/mL,andtheoptimuminductiontimeis30minutes.

  • 标签: Porphyra Mutant of chromatophore N-Methy-N'-Nitro-N-Nitrosoguanidine (NG) thallus
  • 简介:Inthispaper,responsesofanewdual-inductionresistivitylogging-while-drilling(LWD)toolin3Dinhomogeneousformationmodelsaresimulatedbythevectorfiniteelementmethod(VFEM),theinfluencesoftheborehole,invadedzone,surroundingstrata,andtooleccentricityareanalyzed,andcalibrationloopparametersandcalibrationcoefficientsoftheLWDtoolarediscussed.TheresultsshowthatthetoolhasagreaterdepthofinvestigationthanthatoftheexistingelectromagneticpropagationLWDtoolsandismoresensitivetoazimuthalconductivity.Bothdeepandmediuminductionresponseshavelinearrelationshipswiththeformationconductivity,consideringoptimalcalibrationloopparametersandcalibrationcoefficients.Duetothedifferentdepthsofinvestigationandresolution,deepinductionandmediuminductionareaffecteddifferentlybytheformationmodelparameters,therebyhavingdifferentcorrectionfactors.Thesimulationresultscanprovidetheoreticalreferencesfortheresearchandinterpretationofthedual-inductionresistivityLWDtools.

  • 标签: Dual-induction RESISTIVITY LWD tool vector finite