简介：AbstractObjective:To review the role of adjuvant hyperbaric oxygen therapy (HBOT) in the treatment of malignant otitis externa (MOE).Data sources:PubMed, Scopus, Web of Science, Science Direct, and Cochrane Library were searched for the following concepts: "hyperbaric oxygen" and "malignant or necrotizing otitis externa."Methods:Studies were included if they contained (1) patients with reported evidence of MOE, (2) employment of adjuvant HBOT, (3) details on patients’ medical condition, and (4) documented survival outcomes. Extracted information included patient demographics, underlying medical conditions, infectious etiology, signs and symptoms, medical and surgical treatments, duration of medical treatment, mean follow up time, HBOT setting, number of HBOT sessions, complications, survival rate, and all-cause mortality.Results:A total of 16 studies comprising 58 patients (mean age 68.0 years) were included. Diabetes was present in 94.7% of cases and Pseudomonas spp (64.3%) was the most common infectious agent. Cranial nerve VII was involved in 55.2% of cases. Overall, the disease cure rate with adjuvant HBOT was 91.4% and all-cause mortality was 8.6%. Among those who had cranial nerve VII involvement, 72.0% had return of function and 93.8% of them survived.Conclusion:HBOT may be an effective treatment option for refractory or advanced MOE but its efficacy remains unproven due to lack of strong scientific evidence. However, its therapeutic value should not be underestimated given good results and few adverse events reported in this study.

简介：摘要Background and objectiveCloser monitoring and treatment is vital for pregnant carbon monoxide (CO) poisoning cases due to fetal poisoning component. Permanent damage can occur in both the mother and the baby. It may cause stillbirth even though no serious clinical symptoms occur in the mother. Hyperbaric oxygen (HBO) treatment is advised for all pregnant patients regardless of their clinical symptoms. Pregnant CO poisoning patients that received HBO treatment and their fetal status were evaluated in this study.MethodsPregnant patients poisoned with CO treated in the same hyperbaric clinic were evaluated. Pregnant patients that received HBO treatment in a multiplace chamber were evaluated in terms of clinical status, demographic structure, laboratory tests, fetal effects and progress of the fetus until birth and 6 months postpartum.ResultsA total number of 32 pregnant cases were treated. COHb values were over 20% (min 6.9- max 40.2) in 23 patients, 11 patients had a history of syncope. All patients took HBO treatment under 2.4 ATA pressure for 120 min. 3 patients received more than 1 session of HBO treatments due to fetal stress; all other cases took 1 session of HBO treatment. No spontaneous abortus occurred in early follow-ups; only 4 babies were born prematurely. 2 of the babies were lost in the early phases after birth, due to causes non-related to CO poisoning complications (cyanotic heart disease, necrotizing enterocolitis). No significant difference were observed in the comparison of laboratory results of patients with syncope and of those who did not have syncope and comparison of patients with COHb value higher than 20% and patients with COHb value lower than 20% (P>0.05)．ConclusionHBO is not advisable for pregnant patients except for CO poisoning. In this study it is observed that HBO treatment under 2.4 ATA pressure for 120 min has no harmful effects on the mother and the fetus. It is observed that continuation of HBO treatment in the cases with fetal distress findings has beneficial effects. COHb levels and syncope were shown to have no significant effect on clinical symptoms and on blood tests.

简介：瞄准：在试验性的老鼠在生物化学、组织病理学说的变化，氧化压力，和细菌的易位(BT)上调查allopurinol和高比重的氧(HBO)治疗的单个、联合的效果尖锐胰腺炎(AP)。方法：85只Sprague-Dawley老鼠在学习被包括。85只老鼠中的十五个被用作控制(假冒，组我)。AP在留下经由管内牛磺胆酸盐注入被导致七十只老鼠。幸存到尖锐引起坏死胰腺炎的正式就职的老鼠被使随机化进四个组。组II独自收到了saline，组IIIallopurinol，加HBO的组IVallopurinol和组VHBO。浆液淀粉酶层次，氧化压力参数，BT和组织病理学说的分数是坚定的。结果：浆液淀粉酶层次在与组II相比的组III，IV和V是更低的(974+/-110，384+/-40，851+/-56，和1664+/-234U/L，分别地P<0.05，为所有)。联合揭示的二种处理选择显著地降低median[25-75百分位数]组织病理学说的分数什么时候与单个政府相比(13[12.5-15]在allopurinol组，9.5[7-11.75]在HBO组，并且6[4.5-7.5]在联合的组，P<0.01)。氧化压力标记在与控制相比的所有处理组显著地更好。进胰和mes伤寒淋巴结的细菌的易位在与组II相比的组III，IV和V是更低的(54%，23%，50%对为到胰的易位的100%，并且62%，46%，58%对为到mes伤寒淋巴节点的易位的100%，分别地P<0.05为所有)。结论：当在试验性的老鼠AP独立管理了时，现在的学习证实HBO和allopurinol处理的利益。这些处理选择的联合看起来更有效地阻止胰腺的损害参数的前进。

简介：摘要IntroductionHyperbaric oxygen (HBO) therapy may be a useful treatment to prevent the development of delayed neuropsychiatric sequelae (DNS) in patients with acute carbon monoxide (CO) poisoning. However, there is no clear consensus regarding the optimal number of HBO therapy sessions in patients with CO poisoning. Here, we compared the development of DNS after 3 and > 3 sessions of HBO therapy in patients with acute CO poisoning.MethodsThis prospective observational study recruited 299 patients with CO poisoning. Demographic and clinical information were obtained, including comorbidities, vital signs, and symptoms. Patients were divided into two groups according to whether they received 3 or > 3 sessions of HBO therapy (3 HBO vs. >3 HBO). A propensity score-matching process was used to balance potential prognostic factors in both groups.ResultsOf the 299 patients with acute CO poisoning enrolled in this study, 183 (59.0%) were included in the analysis. Patients were excluded for the following reasons: age < 18 years, not underwent HBO therapy, discharged against medical advice, and loss to follow-up. The overall rate of DNS development was 17.5%. The >3 HBO group had a higher incidence of DNS development compared to the 3 HBO group (36.3% vs. 16.3%; P＝0.09). Propensity score-matching analysis revealed similar incidences of DNS (31.3% vs. 28.1%, respectively; P>0.99)．ConclusionThere is a critical need to determine the optimal number of HBO therapy sessions for patients with acute CO poisoning. This study showed no difference in DNS development after 3 and > 3 sessions of HBO therapy.

简介：摘要ObjectiveTo assess real-world effectiveness of hyperbaric oxygen therapy (HBOT) on delayed neuropsychiatric sequelae (DNS) after carbon monoxide (CO) poisoning we conducted a retrospective review of patients with CO poisoning admitted to Linkou Chang-Gung Memorial Hospital, Taiwan′s largest medical center, during 2009-2015.MethodsWe included patients developing DNS after CO poisoning and compared improvements in neuropsychiatric function, with and without HBOT, after 12 months post-DNS to understand differences in recovery rates. DNS improvement-associated factors were also evaluated. We used receiver operating characteristic (ROC) curve analysis to assess the role of time elapsed between DNS diagnosis and HBOT initiation in predicting DNS improvement.ResultsA total of 62 patients developed DNS, of whom 11 recovered while the rest did not. Possible factors predicting DNS improvement included receiving HBOT post-DNS (72.7% vs 25.5%; P＝0.006), and treatment with more than three HBOT sessions during acute stage CO poisoning (81.8% vs 27.5%; P＝0.003). The relevant area under the ROC curve was 0.789 (95% CI 0.603-0.974), and the best cut-off point was 3 days post-DNS diagnosis, with 87.5% sensitivity and 61.5% specificity.ConclusionEarly HBOT in patients who developed DNS after CO poisoning significantly improved their DNS symptoms, with treatment effects sustained for 1 year after DNS diagnosis.

简介：Objective:Toevaluatetheeffectsofhyperbaricoxygen(HBO)therapyonpatientswithpostbraininjuryneuralstatus.Methods:Twoto4coursesofHBOtherapyand/ormedicationswereusedtotreat320patientswhowererandomlydividedintotwogroups.Assessmentwasmadewith99mTc-ethylcysteinatedimer(99mTc-ECD)singlephotonemissioncomputedtomography(SPECT)beforeandaftertreatment.Results:TherewasasignificantdifferencebetweentheHBOtherapygroupandthenon-HBOtherapygroup.HBOtherapywassuperiortomedicationtreatmentaloneintherecoveryofclinicalsymptoms,controlofepilepsy,andresolutionofhydrocephalus(P<0.01).Conclusions:HBOtherapyhasspecificcurativeeffectsonpatientswithpostbraininjuryneuralstatus,and99mTc-ECDSPECTcouldplayanimportantroleindiagnosingpostbraininjuryneuralstatusandmonitoringthetherapeuticeffectsofHBO.

简介：摘要ObjectiveThis study aimed at assessing which one of the 2 therapies is better for treating carbon monoxide (CO) poisoning from the perspective of reducing delayed neuropsychologicsequelae (DNS).MethodsWe used Taiwan′s National Health Insurance Research Database (NHIRD) to conduct a nationwide population-based cohort study to assess which therapy is better for CO poisoning patients. To accurately identify patients with DNS, the definition of DNS is included neurological sequelae, and cognitive and psychological sequele. The independent variable was therapy and the dependent variable was DNS occurred within 1 year after discharge from a medical institution. The control variables were age, gender, the severity of CO poisoning, and comorbidities present before CO poisoning admission.ResultsThe risk of developing DNS in patients treated with Hyperbaric Oxygen (HBO) was 1.87-fold (P< .001) than normobaric oxygen (NBO) therapy. The severity of CO poisoning and comorbidities were also found to have significant influences on the risk of developing DNS.ConclusionsHBO may be a risk therapy for treating CO poisoning.

简介：摘要ObjectivesDelayed neuropsychiatric sequelae (DNS) are serious complications of carbon monoxide poisoning; neuropsychiatric disorders can occur within a few days of recovery from acute poisoning. Hyperbaric oxygen therapy (HBOT) has been the main treatment of carbon monoxide (CO) poisoning and was recommended as the treatment choice for CO poisoning by the American Undersea and Hyperbaric Medical Society and the Tenth European Consensus Conference on Hyperbaric Medicine of the European Underwater and Baromedical Society. However, the optimal timing for commencing HBOT in patients with CO poisoning remains unknown. We therefore conducted a retrospective study in an attempt to target the optimal time of HBOT for DNS prevention.MethodsA retrospective review of patient files/medical records was conducted on all patients with CO poisoning admitted to the Emergency Department of Linkou Chang-Gung Memorial Hospital, Taiwan between January 1, 2009 and December 31, 2015. A total of 279 patients who received HBOT were eligible for further DNS detection. DNS was defined as the presence of one of the following neurological, cognitive, or psychological sequelae that were documented in the medical record during hospital stay or outpatient clinic follow-up for at least 6 months. A multivariable logistic regression analysis was employed to identify potential determinants of DNS after receiving HBOT for CO poisoning. A receiver operating characteristic (ROC) curve was used to analyse the influence of duration from CO exposure to HBOT on DNS development.ResultsA Glasgow coma score of <9 (odds ratio [OR], 3.20; 95% confidence interval [CI], 1.19-8.60) and a longer duration from CO exposure to HBOT (OR, 1.06; 95% CI, 1.03-1.09) were associated with a higher risk of DNS. By contrast, the presence of multiple victims from the same incident was associated with a lower risk of DNS. The ROC curve for the duration between CO exposure and HBOT in predicting DNS development demonstrated an area under the curve of 0.638 (95% CI, 0.575-0.698). The optimal cut-off point according to the Youden index was 22.5 h, with a sensitivity of 41.7% and a specificity of 85.9%. We also stratified the duration from CO exposure to HBOT into 5 intervals (< 6 h, 6-11 h, 12-23 h, 24-47 h and ≥ 48 h) and revealed a trend of increasing DNS risk with time.ConclusionWe identified several potential predictors of DNS in patients with CO poisoning who received HBOT. Multivariable logistic regressions further revealed that longer duration from CO exposure to HBOT, loss of consciousness, and the presence of multiple victims were independent predictors of DNS development. HBOT should be performed as early as possible and preferably within 22.5 h after CO poisoning.

简介：Yifeng(TE17),Tinghui(GB2),Zhongzhu(TE3)andXiaxi(GB43)wereselectedaskeyacupointsplushyperbaricoxygentotreat50casesofsuddendeafness,andthetotaleffectiveratewas98%.

简介：AbstractBackground:Hyperbaric oxygen treatment (HBOT) has been demonstrated to influence the keloid recurrence rate after surgery and to relieve keloid symptoms and other pathological processes in keloids. To explore the mechanism of the effect of HBOT on keloids, tumor immune gene expression and immune cell infiltration were studied in this work.Methods:From February 2021 to April 2021, HBOT was carried out on keloid patients four times before surgery. Keloid tissue samples were collected and divided into an HBOT group (keloid with HBOT before surgery [HK] group, n = 6) and a non-HBOT group (K group, n = 6). Tumor gene expression was analyzed with an Oncomine Immune Response Research Assay kit. Data were mined with R package. The differentially expressed genes between the groups were compared. Hub genes between the groups were determined and verified with Quantitative Real-time PCR. Immune cell infiltration was analyzed based on CIBERSORT deconvolution algorithm analysis of gene expression and verified with immunohistochemistry (IHC).Results:Inflammatory cell infiltration was reduced in the HK group. There were 178 upregulated genes and 217 downregulated genes. Ten hub genes were identified, including Integrin Subunit Alpha M (ITGAM), interleukin (IL)-4, IL-6, IL-2, Protein Tyrosine Phosphatase Receptor Type C (PTPRC), CD86, transforming growth factor (TGF), CD80, CTLA4, and IL-10. CD80, ITGAM, IL-4, and PTPRC with significantly downregulated expression were identified. IL-10 and IL-2 were upregulated in the HK group but without a significant difference. Infiltration differences of CD8 lymphocyte T cells, CD4 lymphocyte T-activated memory cells, and dendritic resting cells were identified with gene CIBERSORT deconvolution algorithm analysis. Infiltration levels of CD4 lymphocyte T cell in the HK group were significantly higher than those of the K group in IHC verification.Conclusion:HBOT affected tumor gene expression and immune cell infiltration in keloids. CD4 lymphocyte T cell, especially activated memory CD4+T, might be the key regulatory immune cell, and its related gene expression needs further study.

简介：摘要BackgroundIt is important to prevent the development of delayed neuropsychiatric sequelae (DNS) in acute carbon monoxide (CO) intoxication, but no effective treatment has been clearly identified. Hyperbaric oxygen (HBO) therapy is one of the treatment options in acute CO poisoning; however, whether it can prevent the development of DNS is controversial.ObjectiveThe purpose of this study is to compare the effectiveness of normobaric oxygen (NBO) and HBO in preventing DNS.MethodsThis prospective observational study was conducted on all patients with CO poisoning admitted to the emergency department of a tertiary hospital from 2016 to 2019. We followed-up patients to determine whether symptoms of DNS occurred at ≤6 months. We matched the propensity score to an equivalent distribution of potential covariates.ResultsA total of 224 patients with CO poisoning were enrolled in this study. NBO was used for 26 patients and HBO for 198 patients. DNS occurred in 40 patients. There were significant differences between the NBO and HBO groups in terms of carboxyhemoglobin, loss of consciousness, dizziness, chest pain, hospitalization, and length of hospital stay. The incidence of DNS was 19.2% in the HBO group, which was higher than the 7.7% observed in the NBO group, but the difference was not significant (P＝0.18). After propensity score matching, the incidence of DNS did not differ between the NBO and HBO groups (8.3% vs. 10.4%, P>0.99)．ConclusionThere was no difference in the incidence of DNS between groups receiving HBO and NBO in acute CO intoxication.

简介：Objective:Toinvestigateifhyperbaricoxygen(HBO)mayinducestructuralchangesofneuronsinhippocampusfrominfactileratsandifthechangesarereversible.Methods:All27healthySDinfantileratswereexposedtoHBO(0.25MPa)orhyperbaricair(HBA)for1to3courses(10daysas1course).Thehippocampuswastakenattheendofeachcoursetoobserveitsmorphologybylightmicroscopeandelectronmicroscope.Results:HBOexposureinducedcapillarydilation,nuclearmembranewindingorblurringandsomemitochondriaswellingwithitscristablurringinneurons.Thechangesoccurredafter1courseexposureandbecamesignificantwithtime.Mostofthechangesrecovered20daysafterstoppingexposure.NochangewasfoundafterHBAexposure.Conclusions:Long-termHBOexposurecancausecapillarydilationandultrastructuralinjuryofneuronsinhippocampusfrominfantilerats.Thedamageisnotserious,butreversible.

简介：摘要BackgroundDelayed encephalopathy (DE) is the most severe complication after acute carbon monoxide (CO) poisoning, which seriously affects the outcome of patients and leads to a high disability rate. Prior studies have shown that hyperbaric oxygen (HBO2) therapy is therapeutic for DE due to reducing immune-mediated neuropathology and thus improving cognitive performance.MethodsIn our present perspective study, five DE patients were treated regularly with HBO2 therapy. The mini-mental state examination (MMSE) and Barthel index (BI) were intermittently collected during their hospitalization for mental and physical status evaluation, the peripheral bloods were serially sampled to determine the concentration changes of circulating stem cells, as well as corresponding BDNF and neural markers.ResultsMMSE and BI showed series of improvements after multiple HBO2 therapies. The CD34+/CD90+ and CD34+/CD133+ dual positive cells, which were categorized as circulating stem cells, were observed an overall up-regulation since the beginning of the DE onset upon the application of HBO2 therapy. Characteristic neurotrophin BDNF, neural markers such as nestin and synaptophysin (SYP) were also up-regulated after exposure of HBO2. Conclusion The application of HBO2 therapy is of significance in improving the cognition of DE patients, along with mobilized circulating stem cells.ConclusionWe primarily infer that the CD34+/CD90+ and CD34+/CD133+ cells were mobilized by HBO2 exposure and have played a positive role in cognition improvement on DE patients by up-regulation of BDNF, nestin and SYP. The altering amount of circulating stem cells mobilized in peripheral blood could be a potential marker on predicting the outcome of DE.

简介：摘要Background and purposeCarbon monoxide (CO) is a gas product of combustion, considered highly poisonous. Prolonged CO exposure is responsible for more than half of fatal poisonings and is also one of the leading causes of poisoning in Western countries. We aimed to compare the effectiveness of therapy with hyperbaric oxygen (HBO) versus normobaric oxygen (NBO) in the setting of carbon monoxide poisoning (COP).MethodsWe independently searched the National Library of Medicine′s Medline (PubMed™), ScienceDirect™，and Scielo™ for any relevant studies published from 1989 to 2017, using the following keywords: hyperbaric therapy, hyperbaric oxygenation, normobaric therapy, carbon monoxide poisoning, carboxyhemoglobin, Haldane effect. We analyzed the studies that suggested the effectiveness of HBO or NBO. Also，we searched for studies related to COP; including history，epidemiology (risk factors，incidence，demographics)，pathophysiology, clinical manifestations, diagnosis, and treatment.ResultsSixty-eight articles were found, sixteen of which dealt with either HBO or NBO or both. Twelve suggested HBO as the treatment of choice in COP; four studies indicated that NBO was an adequate treatment due to its cost-effectiveness and availability in the emergency department (ED). HBO has been shown in several studies to be effective in moderate to high-risk COP situations, being the therapy of choice to avoid sequelae, especially neurologically.ConclusionNBO can be considered as a reasonable alternative due to its cost-effectiveness. The availability and understanding of different therapeutic interventions are critical in the management of patients with COP in ED and the Critical Care unit.

简介：Objective:Toexploretheeffectsofhyperbaricoxygen(HBO)treatmentontheneuronalapoptosisatanearlierstageandtheexpressionsofCytochromeC(CytC),Bcl-2(B-celllymphoma-2family)andBax(Bcl-2associatedXprotein)inratbraintissuesaftertraumaticbraininjury(TBI).Methods:Fortyadultratsweredividedintotwogroups,i.e.,GroupA(theratswithuntreatedTBI)andGroupB(ratswithHBOtreatmentafterTBI).Sectionsofbraintissuesofthesetwogroupswerethendetectedat3,6,12,24,72hoursafterTBIbyimmunohistochemistryandelectronmicroscope,respectively.Results:HBOtreatmentcouldup-regulatetheexpressionofBcl-2within72hours,reducethereleaseofCytCfrommitochondria,attenuatetheformationofdimericBaxandalleviatethemitochondrialedemawithin24hoursafterTBI.Conclusions:HBOtreatmentcanalleviateneuronalapoptosisafterTBIbyreducingthereleaseofCytCandthedimersofBaxandup-regulatingtheexpressionofBcl-2.

简介：无

简介：Objective:Tostudythechangesofpartialpressureofbraintissueoxygen(PbtO2)andbraintemperatureinacutephaseofsevereheadinjuryduringmildhypothermiatherapyandtheclinicalsignificance.Methods:Onehundredandsixteenpatientswithsevereheadinjurywereselectedanddividedintoamildhypothermiagroup(n=58),andacontrolgroup(n=58)accordingtooddandevennumbersofhospitalization.WhilemildhypothermiatherapywasperformedPbtO2andbraintemperatureweremonitoredfor1-7days(mean=86hours),simultaneously,theintracranialpressure,rectumtemperature,cerebralperfusionpressure,PaO2andPaCO2werealsomonitored.Thepatientswerefollowedupfor6monthsandtheprognosiswasevaluatedwithGOS(Glasgowoutcomescale).Results:ThemeanvalueofPbtO2within24hourmonitoringinthe116patientswas13.7mmHg±4.94mmHg,lowerthanthenormalvalue(16mmHg±40mmHg)ThetimeofPbtO2recoveringtothenormalvalueinthemildhypothermiagroupwasshortenedby10±4.15hourscomparedwiththecontrolgroup(P<0.05).Thesurvivalrateofthemildhypothermiagroupwas60.43%,higherthanthatofthecontrolgroup(46.55%).Aftertherecoveryofthebraintemperature,PbtO2increasedwiththeriseofthebraintemperature.Conclusions:Mildhypothermiacanimprovethesurvivalrateofsevereheadinjury.ThetechniqueofmonitoringPbtO2andthebraintemperatureissafeandreliable,andhasimportantclinicalsignificanceinjudgingdiseaseconditionandinstructingclinicaltherapy.

简介：霸气是黑色的天性，同oxygen（氧气）一样无处不在。在时尚中多年坚持，水滴石穿，具有阴阳双重性格，一方面是阴柔的、谦逊的、内隐的、舒服的；另一方面是阳刚的、桀骜的、外显的、富于表情的、始终成为流行的主导。

简介：Carbonsupportedgold-iridiumcomposite(AuIr/C)wassynthesizedbyafacileone-stepprocessandwasinvestigatedasthebifunctionalcatalystforoxygenreductionreaction(ORR)andoxygenevolutionreaction(OER).ThephysicalpropertiesoftheAuIr/Ccompositewerecharacterizedbytransmissionelectronmicroscopy(TEM),X-raydiffraction(XRD)andX-rayphotoelectronspectroscopy(XPS).AlthoughtheAuandIrintheAuIr/Cdidnotformalloy,itisclearthattheintroductionofIrdecreasestheaverageAuparticlesizeto4.2nmcomparedtothatintheAu/C(10.1nm).BysystematicalanalysisonchemicalstateofmetalsurfaceviaXPSandtheelectrochemicalresults,itwasfoundthattheAusurfacefortheAu/Ccanbeactivatedbypotentialcyclingfrom0.12Vto1.72V,resultingintheincreasedsurfaceroughnessofAu,thusimprovingtheORRactivity.Bythesamepotentialcycling,theIrsurfaceoftheIr/Cwasirreversiblyoxidized,leadingtodegradedORRactivitybutuninfluencedOERactivity.FortheAuIr/C,IrprotectsAuagainstbeingoxidizedduetothelowerelectronegativityofIr.CombiningtheadvantagesofAuandIrincatalyzingORRandOER,theAuIr/CcatalystdisplaysanenhancedcatalyticactivitytotheORRandacomparableOERactivity.Inthe50-cycleacceleratedagingtestfortheORRandOER,theAuIr/Cdisplayedasatisfiedstability,suggestingthattheAuIr/Ccatalystisapotentialbifunctionalcatalystfortheoxygenelectrode.

简介：基因治疗为癌症的治疗提供一条新途径。编码immunostimulatorycytokines的基因的转移与显著成功被使用了在动物消除癌症。然而，在有这策略的病人的临床的试用限制了功效。因此，基因转移向量系统的改进是必要的。混合病毒的向量，与鼠科的IL-12或记者LacZ基因由SFVreplicon组成，被构造。这混合向量在vitro并且在vivo在HCC显示出表示的特性和高水平。在一个老鼠orthotropic肝肿瘤模型，没有伴随毒性，由有mIL-12基因的混合向量的确定的肿瘤的治疗导致了一项强壮的反肿瘤活动。随后，助手依赖者侵入人体气管粘膜的病菌包含mifepristone(RU486)的向量可诱导的系统被构造为控制并且人的interleukin的肝特定的表示12(hIL-12)(HD-Ad/RUhIL-12)并且鼠标IL-12(mIL-12)(HD-Ad/RUmIL-12)。数据证明hIL-12的高、支撑的浆液层次能被继续RU486的管理达到每12或24h。hIL-12的重复正式就职能被获得在上，至少在HD-Ad/RUhIL-12的单个注射以后的48个星期的一个时期。肝转移与的处理HD-Ad/RUmIL-12，正RU846在所有动物导致了完全的肿瘤回归。然后，不同cytokine基因被插入到有条件的replicative侵入人体气管粘膜的病菌向量(也叫的oncolytic侵入人体气管粘膜的病菌)。在肿瘤房间的侵入人体气管粘膜的病菌的复制将杀死肿瘤房间和版本病毒，它感染包围肿瘤房间。由oncolytic侵入人体气管粘膜的病菌的cytopathic效果和transgene的生物效果的联合将施加强壮的反肿瘤活动。向量的这些新类型可以为癌症基因治疗提供一个有势力和安全工具。